A Binding Resolution

Jones, Alan M.; Sussman, Michael R.

doi:10.1104/pp.109.136606

Cited by 10 publications

(5 citation statements)

References 6 publications

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“…A critical difference between the reports of these putative ABA receptors-GCR2, GTG1/GTG2, CHLHand those of the PYR/PYL/RCAR family is that the evidence for the former candidates has not been independently corroborated. Jones and Sussman (2009) proposed the application of strict biochemical criteria when evaluating the candidacy of a protein as a hormone receptor. It may be difficult for one or even two publications by a single research group to meet all of the desirable criteria these authors list.…”

Section: Other Putative Aba Receptorsmentioning

confidence: 99%

ABA receptors: the START of a new paradigm in phytohormone signalling

Klingler

Batelli

Zhu

2010

Journal of Experimental Botany

249

170

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The phytohormone abscisic acid (ABA) plays a central role in plant development and in plant adaptation to both biotic and abiotic stressors. In recent years, knowledge of ABA metabolism and signal transduction has advanced rapidly to provide detailed glimpses of the hormone's activities at the molecular level. Despite this progress, many gaps in understanding have remained, particularly at the early stages of ABA perception by the plant cell. The search for an ABA receptor protein has produced multiple candidates, including GCR2, GTG1, and GTG2, and CHLH. In addition to these candidates, in 2009 several research groups converged on a novel family of Arabidopsis proteins that bind ABA, and thereby interact directly with a class of protein phosphatases that are well known as critical players in ABA signal transduction. The PYR/PYL/RCAR receptor family is homologous to the Bet v 1-fold and START domain proteins. It consists of 14 members, nearly all of which appear capable of participating in an ABA receptor-signal complex that responds to the hormone by activating the transcription of ABA-responsive genes. Evidence is provided here that PYR/PYL/RCAR receptors can also drive the phosphorylation of the slow anion channel SLAC1 to provide a fast and timely response to the ABA signal. Crystallographic studies have vividly shown the mechanics of ABA binding to PYR/PYL/RCAR receptors, presenting a model that bears some resemblance to the binding of gibberellins to GID1 receptors. Since this ABA receptor family is highly conserved in crop species, its discovery is likely to usher a new wave of progress in the elucidation and manipulation of plant stress responses in agricultural settings.

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Section: Other Putative Aba Receptorsmentioning

confidence: 99%

ABA receptors: the START of a new paradigm in phytohormone signalling

Klingler

Batelli

Zhu

2010

Journal of Experimental Botany

249

170

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“…To confirm these criticisms, we will refer to very recent manuscripts that do not agree with certain publications listed in section 6.1. In the last two years: propose that "A ligand binds to its cognate receptor reversibly, saturably, selectively, and with a stoichiometry of one or more molecules of ligand per molecule protein and the binding is usually heat sensitive and affected by proteases"; 168 150 with the isolation and characterization of GTG1 and GTG2, novel GPCR proteins, may be closer to the coveted ABA receptor(s). However, some experimental criticisms (i.e., protein purification and ABA-binding experiments) still cast a shadow over the protocole carried out by the Pandey group.…”

Section: News Views and Controversiesmentioning

confidence: 99%

Seed dormancy and ABA signaling

Rodríguez-Gacio

Matilla-Vázquez

Matilla

2009

Plant Signaling & Behavior

171

104

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The seed is an important organ of higher plants regarding plant survival and species dispersion. The transition between seed dormancy and germination represents a critical stage in the plant life cycle and it is an important ecological and commercial trait. A dynamic balance of synthesis and catabolism of two antagonistic hormones, abscisic acid (ABA) and giberellins (GAs), controls the equilibrium between seed dormancy and germination. Embryonic ABA plays a central role in induction and maintenance of seed dormancy, and also inhibits the transition from embryonic to germination growth. Therefore, the ABA metabolism must be highly regulated at both temporal and spatial levels during phase of dessication tolerance. On the other hand, the ABA levels do not depend exclusively on the seeds because sometimes it becomes a strong sink and imports it from the roots and rhizosphere through the xylem and/or phloem. All theses events are discussed in depth here. Likewise, the role of some recently characterized genes belonging to seeds of woody species and related to ABA signaling, are also included. Finally, although four possible ABA receptors have been reported, not much is known about how they mediate ABA signalling transduction. However, new publications seem to shown that almost all these receptors lack several properties to consider them as such.

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“…According to Jones and Sussman (reviewed in ref. 5) a receptor binds the agonist reversibly, stereospecifically, selectively, and in a defined stoichiometry. The K D should correspond to concentrations which are able to induce responses when applied in physiological experiments.…”

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confidence: 99%