A bioactive elastin-like recombinamer reduces unspecific protein adsorption and enhances cell response on titanium surfaces

Salvagni, Emiliano; Berguig, Geoffrey Y.; Engel, Elisabeth; Rodríguez‐Cabello, José Carlos; Coullerez, Géraldine; Textor, Marcus; Planell, Josep A.; Gil, F.J.; Aparicio, Conrado

doi:10.1016/j.colsurfb.2013.10.008

Cited by 35 publications

(38 citation statements)

References 30 publications

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“…dipole-dipole interactions, London dispersion forces and hydrogen bonding [39,40]. Both activation treatments incremented the hydroxyl groups formation but PL treatment was the more efficient 18 compared to NA and CT. Also confirmed by the O 1s peak deconvolution, Fig.…”

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confidence: 99%

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Biofunctionalization of REDV elastin-like recombinamers improves endothelialization on CoCr alloy surfaces for cardiovascular applications

Castellanos

Zenses

Grau

et al. 2015

Colloids and Surfaces B: Biointerfaces

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To improve cardiovascular implant success, metal-based stents are designated to modulate endothelial cells adhesion and migration in order to prevent restenosis and late thrombosis diseases. Biomimetic coatings with extra-cellular matrix adhesive biomolecules onto stents surfaces are a strategy to recover a healthy endothelium. However, the appropriate bioactive sequences to selective promote growth of endothelium and the biomolecules surface immobilization strategy remains to be elucidated. In this study, biofunctionalization of cobalt chromium, CoCr, alloy surfaces with elastin-like recombinamers, ELR, genetically modified with an REDV sequence, was performed to enhance metal surfaces endothelialization. Moreover, physical adsorption and covalent bonding were used as biomolecules binding strategies onto CoCr alloy. Surfaces were activated with plasma and etched with sodium hydroxide previous to silanization with 3-chloropropyltriethoxysilane and functionalized with the ELR. CoCr alloy surfaces were successfully biofunctionalized and the use of an ELR with an REDV sequence, allows conferring bioactivity to the biomaterials surface, demonstrating a higher cell adhesion and spreading of HUVEC cells on the different CoCr surfaces. This effect is emphasized as increases the amount of immobilized biomolecules and directly related to the immobilization technique, covalent bonding, and the increase of surface charge electronegativity. Our strategy of REDV elastin-like recombinamers immobilization onto CoCr alloy surfaces via covalent bonding through organosilanes provides a bioactive surface that promotes endothelial cell adhesion and spreading.Response to Reviewers: Responses to reviewer's comments:We are grateful to reviewers for the critical comments and useful suggestions that have helped us to improve our paper. As indicated in the responses that follow, we have taken all these comments and suggestions into account in the revised version of our paper. Answers to the Reviewers' comments are written in blue. The main text is revised according to these answers and written in red. AbstractTo improve cardiovascular implant success, metal-based stents are designated to modulate endothelial cells adhesion and migration in order to prevent restenosis and late thrombosis diseases. Biomimetic coatings with extra-cellular matrix adhesive biomolecules onto stents surfaces are a strategy to recover a healthy endothelium. However, the appropriate bioactive sequences to selective promote growth of endothelium and the biomolecules surface immobilization strategy remains to be elucidated. In this study, biofunctionalization of cobalt chromium, CoCr, alloy surfaces with elastin-like recombinamers, ELR, genetically modified with an REDV sequence, was performed to enhance metal surfaces endothelialization. Moreover, physical adsorption and covalent bonding were used as biomolecules binding strategies onto CoCr alloy. Surfaces were activated with plasma and etched with sodium hydroxide previous to silanization with 3-chlor...

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confidence: 99%

“…The designed ELR present alternatively along the chain, the (VPGKG) peptide unit which exhibits a primary amine 8 that may serve for covalent immobilization through a nucleophilic attack [39] or aid for physisorption where it is 9 induced weak bonding, i.e. dipole-dipole interactions, London dispersion forces and hydrogen bonding [39,40].…”

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confidence: 99%

Biofunctionalization of REDV elastin-like recombinamers improves endothelialization on CoCr alloy surfaces for cardiovascular applications

Castellanos

Zenses

Grau

et al. 2015

Colloids and Surfaces B: Biointerfaces

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“…The hydroxyapatite action would cause that surrounding connective tissue became in osteoid tissue, probably due to the action of osteoblasts from healthy areas that colonizing the fibrous capsule and they perform their osteoinductive function onto the fibrous material [6] [6] This means, that the performance of these 3-D biomaterials, when implanted in living tissue, is variable depending on the nature of peptides that are functionalized with. The use of Elastin-like Recombinamers (ELRs) for functionalizing surfaces is a novel advance that has been recently proposed [7] [7]. Among the bio-produced polymers, the ELRs, are considered a family with a high potential for biomaterials design.…”

Section: Thus Vallet Et Al Have Designed a Biomaterials (Ha) For Usementioning

confidence: 99%

3D silicon doped hydroxyapatite scaffolds decorated with Elastin-like Recombinamers for bone regenerative medicine

et al. 2016

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Abstract.The current study reports on the manufacturing by rapid prototyping technique of threedimensional (3D) scaffolds based on silicon substituted hydroxyapatite with Elastin-like Recombinamers (ELRs) functionalized surfaces. Silicon doped hydroxyapatite (Si-HA), with Ca 10 (PO 4 ) 5.7 (SiO 4 ) 0.3 (OH) 1.7 h 0.3 nominal formula, was surface functionalized with two different types of polymers designed by genetic engineering: ELR-RGD that contain cell attachment specific sequences and ELR-SN A 15/RGD with both hydroxyapatite and cells domains that interact with the inorganic phase and with the cells, respectively. These hybrid materials were subjected to in vitro assays in order to clarify if the ELRs coating improved the well-known biocompatible and bone regeneration properties of calcium phosphates materials. The in vitro tests shown that there was a total and homogeneous colonization of the 3D scaffolds by Bone marrow Mesenchymal Stromal Cells (BMSCs). In addition, the BMSCs were viable and able to proliferate and differentiate into osteoblasts. Statement of significanceBone tissue engineering is an area of increasing interest because its main applications are directly related to the rising life expectancy of the population, which promotes higher rates of several bone pathologies, so innovative strategies are needed for bone tissue regeneration therapies. Here we use the rapid prototyping technology to allow moulding ceramic 3D scaffolds and we use different bio-polymers for the functionalization of their surfaces in order to enhance the biological response.Combining the ceramic material (silicon doped hydroxyapatite, Si-HA) and the Elastinlike Recombinamers (ELRs) polymers with the presence of the integrin-mediate 3 adhesion domain alone or in combination with SN A 15 peptide that possess high affinity for hydroxyapatite, provided an improved Bone marrow Mesenchymal Stromal Cells (BMSCs) differentiation into osteoblastic linkage.

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“…17 One of the approaches used in dentistry to covalently immobilize biomolecules on inorganic materials involves methodologies based on silane-chemistry. [18][19][20] Biomolecules with varied complexities like arginine-glycin-aspartate (RGD) sequence , 21,22 other oligopeptides, 23,24 proteins, [25][26][27] aptamers, 28 recombinamers 13,29 or even multiple peptides with cooperative activities 30 have been tethered to metal surfaces using silanes as coupling agents. We have extended this approach to develop mechanically and thermochemically stable biofunctional coatings on titanium which resist surgical shear stresses during implantation as well as the challenging bioenvironments derived from the contact with physiological fluids.…”

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confidence: 99%

“…4,5 Surface functionalization is currently considered as an effective and modern approach to design new multifunctional materials by changing composition, structure and/or morphology of surfaces without altering the bulk properties. 6,7 Physical (increased roughness 8,9 ), chemical (deposition of calcium phosphate phases, chemical etching or incorporation of specific ionic species 10,11 ) and/or biological (bioinspired coatings 12,13 ) approaches can be pursued to functionalize surfaces. Specifically, covalent bonding of biomolecules at surfaces has become pivotal to induct bioactivity on biomaterials [14][15][16] and also for other strategies like assay technologies, biosensors, imaging devices, therapies, etc.…”

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confidence: 99%

Peptide-functionalized zirconia and new zirconia/titanium biocermets for dental applications

Fernández-García¹,

Chen²,

Gutiérrez-González³

et al. 2015

Journal of Dentistry

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A bioactive elastin-like recombinamer reduces unspecific protein adsorption and enhances cell response on titanium surfaces

Cited by 35 publications

References 30 publications

Biofunctionalization of REDV elastin-like recombinamers improves endothelialization on CoCr alloy surfaces for cardiovascular applications

Biofunctionalization of REDV elastin-like recombinamers improves endothelialization on CoCr alloy surfaces for cardiovascular applications

3D silicon doped hydroxyapatite scaffolds decorated with Elastin-like Recombinamers for bone regenerative medicine

Peptide-functionalized zirconia and new zirconia/titanium biocermets for dental applications

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