A biocompatible dual-AIEgen system without spectral overlap for quantitation of microbial viability and monitoring of biofilm formation

He, Wei; Zheng, Zheng; Bai, Haotian; Xiong, Ling‐Hong; Wang, Lei; Li, Yinghui; Kwok, Ryan T. K.; Lam, Jacky W. Y.; Hu, Qinghua; Cheng, Jinquan; Tang, Ben Zhong

doi:10.1039/d1mh00149c

Cited by 7 publications

(6 citation statements)

References 53 publications

(64 reference statements)

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“…As the formation of the network is reversible, Li et al ( 2020) designed a method for phenols pollutant detection because phenols could destroy the network, release TPEDB, and quench the fluorescence. Combining TPEDB with a new NIR-emissive AIEgen DCQA, a dual-AIEgen system without spectral overlap has been designed and fabricated for microbial viability quantification and biofilm formation monitoring (He et al, 2021). DCQA possesses the ability to stain all kinds of microbes, but TPEDB is only capable of recognizing specific dead.…”

Section: Organic Moleculesmentioning

confidence: 99%

Aggregates‐based fluorescence sensing technology for food hazard detection: Principles, improvement strategies, and applications

Chen

Sun

2023

Comp Rev Food Sci Food Safe

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Aggregates often exhibit modified or completely new properties compared with their molecular elements, making them an extraordinarily advantageous form of materials. The fluorescence signal change characteristics resulting from molecular aggregation endow aggregates with high sensitivity and broad applicability. In molecular aggregates, the photoluminescence properties at the molecular level can be annihilated or elevated, leading to aggregation‐causing quenching (ACQ) or aggregation‐induced emission (AIE) effects. This change in photoluminescence properties can be intelligently introduced in food hazard detection. Recognition units can combine with the aggregate‐based sensor by joining the aggregation process, endowing the sensor with the high specificity of analytes (such as mycotoxins, pathogens, and complex organic molecules). In this review, aggregation mechanisms, structural characteristics of fluorescent materials (including ACQ/AIE‐activated), and their applications in food hazard detection (with/without recognition units) are summarized. Because the design of aggregate‐based sensors may be influenced by the properties of their components, the sensing mechanisms of different fluorescent materials were described separately. Details of fluorescent materials, including conventional organic dyes, carbon nanomaterials, quantum dots, polymers and polymer‐based nanostructures and metal nanoclusters, and recognition units, such as aptamer, antibody, molecular imprinting, and host–guest recognition, are discussed. In addition, future trends of developing aggregate‐based fluorescence sensing technology in monitoring food hazards are also proposed.

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Section: Organic Moleculesmentioning

confidence: 99%

Aggregates‐based fluorescence sensing technology for food hazard detection: Principles, improvement strategies, and applications

Chen

Sun

2023

Comp Rev Food Sci Food Safe

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“…Bearing virtues of the unique aggregationintensified fluorescence with brilliant photostability, large Stokes shift, high brightness, and profitable wavelength adjustability, AIEgens exhibit outstanding potential as the qualified fluorescent agents for biofilm imaging or even selectively discrimination because multiple functional substituents could be readily introduced according to the requirements. [49,[57][58][59][60][61][62][63] In this part, the recent advancements in biofilm microenvironment (e.g., enzyme)-responsive imaging, [64] discriminative imaging of Gram-positive bacterial biofilms, [65] and biofilm viability monitoring [66] based on AIEgens are summarized.…”

Section: Bacterial Biofilm Imagingmentioning

confidence: 99%

“…Recently, a dual‐AIEgen system was reported by He et al for morphological imaging and metabolic state detection of microbes and their biofilm aggregates ( Figure a). [ 66 ] This system consisted of a newly synthesized AIEgen, termed DCAQ with near‐infrared (NIR) emission pearked at 729 nm (Figure 4b,c). Owing to the AIE feature, large Stokes shift, NIR emission, and two positive charges, DCAQ was able to stain all kinds of microbes, including Gram‐positive bacteria, Gram‐negative bacteria, and fungi in both live and dead forms.…”

Section: Bacterial Biofilm Imagingmentioning

confidence: 99%

“…Depending on the different characteristics at different states of the biofilm's life cycle, the antibiofilm strategies are diverse. [66,99] For instance, antiadhesion intervention is employed to block the adhesion of microbes to the surfaces in the preliminary stage, which is an effective way to prevent the biofilm's formation with less drug resistance caused. [97][98][99] Regarding to the mature biofilm that has been formed, the therapeutic approaches focus on the degradation of EPS and the eradication of the internal microcolonies.…”

Section: Antibiofilm Theranosticsmentioning

confidence: 99%

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Emerging Applications of Aggregation‐Induced Emission Luminogens in Bacterial Biofilm Imaging and Antibiofilm Theranostics

Liu

Kang

et al. 2023

Small Structures

Self Cite

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Threats posed by recalcitrant bacterial biofilms have been continuously challenging the public health due to the dramatically magnified antibiotic resistance resulted from the complicated biofilm microenvironment. Urgent demands on effective biofilm combating have propelled the rapid exploration of high‐performance antibiofilm systems. Thanks to the distinguished features of aggregation‐intensified fluorescence and aggregation‐enhanced generation of reactive oxygen species, aggregation‐induced emission luminogens (AIEgens) are becoming increasingly eye‐catching in the field of biofilm combating by serving as excellent fluorescence imaging probes or theranostic agents. This review aims to, for the first time, outline the current progress of AIEgens in bacterial biofilm imaging and antibiofilm theranostics. The up‐to‐date advancements of AIEgens in enzyme‐responsive biofilm imaging, discriminative imaging of Gram‐positive bacterial biofilm, as well as biofilm viability monitoring are summarized at first. Subsequently, the antiadhesion intervention‐mediated prevention of biofilm formation and photodynamic therapy‐involved eradication of preexisting biofilms are detailedly elucidated. Finally, a brief conclusion as well as a discussion on the current challenges and future expectations is presented.

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“…Fluorescence imaging is based on the detection of electromagnetic radiation in the ultraviolet–visible–near-infrared (UV–Vis–NIR) range emitted by fluorophores ( Ximendes et al, 2021 ). Thanks to the advantages of high sensitivity, tunable specificity, controllable cost, acceptable biosafety, and noninvasiveness ( Fatahi et al, 2020 ; Guan et al, 2020 ; Niu et al, 2020 ; Guo et al, 2021 ; He et al, 2021 ), fluorescence imaging has been applied to visualize vascular system ( Ma et al, 2018 ; Zheng et al, 2019 ), lymphatic system ( Nakajima et al, 2018 ), various tumor tissues ( Bai et al, 2020 ; Bai et al, 2021 ), and real-time blood flow ( Hong et al, 2014 ; Wan et al, 2018 ; Cai et al, 2020 ). In addition, fluorescence imaging shows great potential in facilitating the development of precise therapeutic strategies for diseases, including imaging-guided cell therapy, drug delivery/release monitoring, and photodynamic therapy ( Li et al, 2020 ; Li et al, 2019a ; Liu et al, 2019 ; Han et al, 2021 ; Xu et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Fluorescence Imaging of Pulmonary Fibrosis in Animal Models

Liu

Tang

Zhu

et al. 2021

Front. Mol. Biosci.

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Pulmonary fibrosis (PF) is a lung disease that may cause impaired gas exchange and respiratory failure while being difficult to treat. Rapid, sensitive, and accurate detection of lung tissue and cell changes is essential for the effective diagnosis and treatment of PF. Currently, the commonly-used high-resolution computed tomography (HRCT) imaging has been challenging to distinguish early PF from other pathological processes in the lung structure. Magnetic resonance imaging (MRI) using hyperpolarized gases is hampered by the higher cost to become a routine diagnostic tool. As a result, the development of new PF imaging technologies may be a promising solution. Here, we summarize and discuss recent advances in fluorescence imaging as a talented optical technique for the diagnosis and evaluation of PF, including collagen imaging, oxidative stress, inflammation, and PF-related biomarkers. The design strategies of the probes for fluorescence imaging (including multimodal imaging) of PF are briefly described, which can provide new ideas for the future PF-related imaging research. It is hoped that this review will promote the translation of fluorescence imaging into a clinically usable assay in PF.

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A biocompatible dual-AIEgen system without spectral overlap for quantitation of microbial viability and monitoring of biofilm formation

Abstract: Lacking rapid and reliable microbial detection and sensing platforms, insufficient understanding towards microbial behaviors may generated or delayed precautions could be made, which greatly threatens human life and increase heavy...

Cited by 7 publications

References 53 publications

Aggregates‐based fluorescence sensing technology for food hazard detection: Principles, improvement strategies, and applications

Aggregates‐based fluorescence sensing technology for food hazard detection: Principles, improvement strategies, and applications

Emerging Applications of Aggregation‐Induced Emission Luminogens in Bacterial Biofilm Imaging and Antibiofilm Theranostics

Recent Advances in Fluorescence Imaging of Pulmonary Fibrosis in Animal Models

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