A biodegradable nanofiber scaffold by electrospinning and its potential for bone tissue engineering

Yoshimoto, Hiroshi; Shin, Yu-Shik; Terai, Hidetomi; Vacanti, Joseph P.

doi:10.1016/s0142-9612(02)00635-x

Cited by 1,850 publications

(1,182 citation statements)

References 11 publications

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“…Calcium was deposited according to cell orientation on PCL fibers. This observation is in agreement with previously published studies where random and aligned PCL nanofibers have been shown to promote osteogenesis 10, 11, 12, 22. Furthermore, MSC cultured on TCP have been shown to undergo osteogenic differentiation when cultured in osteogenic medium 40.…”

Section: Discussionsupporting

confidence: 93%

“…Bone matrix forms a nanometer‐sized composite of organic and inorganic materials with proteins in the range of 50–500 nm 30, 31. Electrospinning has been widely used to fabricate nanoscale substrates 10, 11, 12, 17, 18, 22, 28. Here, hBMSC orientation and morphology followed the orientation of aligned and random PCL nanofibers.…”

Section: Discussionmentioning

confidence: 99%

“…Due to its cost‐effectiveness and versatility electrospinning has been utilized for the fabrication of fibrous scaffolds with diameters between nanometers to several micrometers 6. Commonly used materials for electrospinning are poly (ɛ‐caprolactone) (PCL) and PCL‐composites, which are attractive for bone tissue regeneration due to their mechanical properties, slow degradation rate, low cost and lack of toxicity 10, 11, 12. Due to their dimension and fibrous structure electrospun nanofibers resemble the native bone matrix and are thought to be an ideal artificial matrix to provide cues for the formation of calcified bone tissue 13, 14.…”

Section: Introductionmentioning

confidence: 99%

“…Bone marrow stromal cells and osteoblast‐like cells exhibited higher proliferation and differentiation as well as an increased alkaline phosphatase (ALP) production on sub‐micrometer sized fibers compared with micrometer‐scaled fiber meshes 10, 11, 16…”

Section: Introductionmentioning

confidence: 99%

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Hydrostatic pressure in combination with topographical cues affects the fate of bone marrow‐derived human mesenchymal stem cells for bone tissue regeneration

Reinwald

Haj

2017

J Biomedical Materials Res

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Topographical and mechanical cues are vital for cell fate, tissue development in vivo, and to mimic the native cell growth environment in vitro. To date, the combinatory effect of mechanical and topographical cues as not been thoroughly investigated. This study investigates the effect of PCL nanofiber alignment and hydrostatic pressure on stem cell differentiation for bone tissue regeneration. Bone marrow‐derived human mesenchymal stem cells were seeded onto standard tissue culture plastic and electrospun random and aligned nanofibers. These substrates were either cultured statically or subjected to intermittent hydrostatic pressure at 270 kPa, 1 Hz for 60 min daily over 21 days in osteogenic medium. Data revealed higher cell metabolic activities for all mechanically stimulated cell culture formats compared with non‐stimulated controls; and random fibers compared with aligned fibers. Fiber orientation influenced cell morphology and patterns of calcium deposition. Significant up‐regulation of Collagen‐I, ALP, and Runx‐2 were observed for random and aligned fibers following mechanical stimulation; highest levels of osteogenic markers were expressed when hydrostatic pressure was applied to random fibers. These results indicate that fiber alignment and hydrostatic pressure direct stem cell fate and are important stimulus for tissue regeneration. © 2017 The Authors Journal of Biomedical Materials Research Part A Published by Wiley Periodicals, Inc. J Biomed Mater Res Part A: A: 629–640, 2018.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hydrostatic pressure in combination with topographical cues affects the fate of bone marrow‐derived human mesenchymal stem cells for bone tissue regeneration

Reinwald

Haj

2017

J Biomedical Materials Res

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“…These fibers are exceedingly long (km range) compared with their diameters (x), which usually follow a unimodal statistical distribution. The mean and spread of such distributions can be controlled and tweaked via process parameters over a wide range, from a few nanometers to hundreds of microns [10][11][12][13][14][15][16][17][18][19][20][21][22][23]. The fiber diameter x is regarded as the prime controllable design parameter to steer scaffold performance in terms of cell response.…”

Section: Introductionmentioning

confidence: 99%

Multiscale three-dimensional scaffolds for soft tissue engineering via multimodal electrospinning

et al. 2010

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a b s t r a c tA novel (scalable) electrospinning process was developed to fabricate bio-inspired multiscale threedimensional scaffolds endowed with a controlled multimodal distribution of fiber diameters and geared towards soft tissue engineering. The resulting materials finely mingle nano-and microscale fibers together, rather than simply juxtaposing them, as is commonly found in the literature. A detailed proof of concept study was conducted on a simpler bimodal poly(e-caprolactone) (PCL) scaffold with modes of fiber distribution at 600 nm and 3.3 lm. Three conventional unimodal scaffolds with mean diameters of 300 nm and 2.6 and 5.2 lm, respectively, were used as controls to evaluate the new materials. Characterization of the microstructure (i.e. porosity, fiber distribution and pore structure) and mechanical properties (i.e. stiffness, strength and failure mode) indicated that the multimodal scaffold had superior mechanical properties (Young's modulus $40 MPa and strength $1 MPa) in comparison with the controls, despite the large porosity ($90% on average). A biological assessment was conducted with bone marrow stromal cell type (mesenchymal stem cells, mTERT-MSCs). While the new material compared favorably with the controls with respect to cell viability (on the outer surface), it outperformed them in terms of cell colonization within the scaffold. The latter result, which could neither be practically achieved in the controls nor expected based on current models of pore size distribution, demonstrated the greater openness of the pore structure of the bimodal material, which remarkably did not come at the expense of its mechanical properties. Furthermore, nanofibers were seen to form a nanoweb bridging across neighboring microfibers, which boosted cell motility and survival. Lastly, standard adipogenic and osteogenic differentiation tests served to demonstrate that the new scaffold did not hinder the multilineage potential of stem cells.

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Multilayer Cell-Seeded Polymer Nanofiber Constructs for Soft-Tissue Reconstruction

Barker

Bowers

Hughley

et al. 2013

JAMA Otolaryngol Head Neck Surg

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Polymer nanofiber-based scaffolds mimic natural extracellular matrix. Preseeding the nanofiber construct with cells improved vascularization without notable effects on volume. An effect of cell preseeding on scaffold vascularization was evident beyond the presence of preseeded cells. This 3-dimensional, multilayer method of cell seeding throughout a 1-mm-thick construct is simple and feasible for clinical application. Further development of this technique may affect the clinical practice of facial plastic and reconstructive surgeons.

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A biodegradable nanofiber scaffold by electrospinning and its potential for bone tissue engineering

Cited by 1,850 publications

References 11 publications

Hydrostatic pressure in combination with topographical cues affects the fate of bone marrow‐derived human mesenchymal stem cells for bone tissue regeneration

Hydrostatic pressure in combination with topographical cues affects the fate of bone marrow‐derived human mesenchymal stem cells for bone tissue regeneration

Multiscale three-dimensional scaffolds for soft tissue engineering via multimodal electrospinning

Multilayer Cell-Seeded Polymer Nanofiber Constructs for Soft-Tissue Reconstruction

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