A biodegradable scaffold enhances differentiation of embryonic stem cells into a thick sheet of retinal cells

Singh, Deepti; Wang, Shao-Bin; Xia, Tina; Tainsh, Laurel T.; Ghiassi-Nejad, Maryam; Xu, Tao; Peng, Shaomin; Adelman, Ron A.; Rizzolo, Lawrence J.

doi:10.1016/j.biomaterials.2017.10.052

Cited by 51 publications

(25 citation statements)

References 61 publications

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“…Thus, the novel decellularized “book” scaffolds combined with BMSCs sheets have significant effect on tendon healing. Prior studies have shown that stem cells can be differentiated into specific tissue in different inductive microenvironment, and decellularized tendon scaffolds can induce stem cells to differentiate toward tendon . TNMD and COL1A1 are important indicators for evaluating tenogenic differentiation of BMSCs .…”

Section: Discussionmentioning

confidence: 99%

“…Prior studies have shown that stem cells can be differentiated into specific tissue in different inductive microenvironment, and decellularized tendon scaffolds can induce stem cells to differentiate toward tendon. [10][11][12][13][14] TNMD and COL1A1 are important indicators for evaluating tenogenic differentiation of BMSCs. [24][25][26][27][28] In the current study, results of qRT-PCR and imunofluorescent analysis indicated that the DTS could induce BMSCs to differentiate toward tendon, which may result from the scaffold microenvironment.…”

Section: Discussionmentioning

confidence: 99%

“…Acellular biological scaffolds and stem cells are widely used in tendon tissue reconstruction recently, either alone or in combination . Acellular biological scaffold has reduced the immunogenicity of allografts because of removal of cell components of the tissue, and the physiological structure and extracellular matrix components have been well reserved, the natural biomimetic microenvironment provides important regulatory role for stem cells proliferation and differentiation …”

mentioning

confidence: 99%

“…[7][8][9] Acellular biological scaffold has reduced the immunogenicity of allografts because of removal of cell components of the tissue, and the physiological structure and extracellular matrix components have been well reserved, the natural biomimetic microenvironment provides important regulatory role for stem cells proliferation and differentiation. [10][11][12][13][14] The combination of scaffolds and stem cells has been carried out using high-density cell suspensions traditionally. However, this method can lead to cell loss, uneven cell distribution, and lack of cells in the peripheral part of the scaffold easily.…”

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confidence: 99%

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Book‐shaped decellularized tendon matrix scaffold combined with bone marrow mesenchymal stem cells‐sheets for repair of achilles tendon defect in rabbit

Xie

Zhou

Tang

et al. 2019

Journal Orthopaedic Research

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Tissue‐engineering approaches have great potential to improve the treatment of tendon injuries which are major musculoskeletal disorders. The purpose of this study was to assess the tissue engineering potential of a novel multilayered decellularized tendon “book” scaffold with bone marrow mesenchymal stem cells (BMSCs) sheets for repair of an Achilles tendon defect in a rabbit model. In this study, we developed a novel book‐shaped decellularized scaffold derived from the extracellular matrix of tendon tissues from New Zealand white rabbits. Hematoxylin and eosin (H&E) staining, 4′, 6‐diamidino‐2‐phenylindole (DAPI) staining, DNA quantitation, and scanning electron microscopy (SEM) confirmed the efficiency of decellularization. After culturing BMSCs on decellularized scaffolds, 3‐(4, 5‐dimethylthiazol‐2‐yl)‐2, 5‐diphenyltetrazolium bromide (MTT) assay, SEM, quantitative real time polymerase chain reaction (qRT‐PCR), and immunofluorescence analysis demonstrated that decellularized scaffolds have the capacity to yield homogeneous distribution and alignment of BMSCs, as well as support their differentiation into tendon. Tenomodulin and Alpha‐1 collagen type I are important indicators for evaluating tenogenic differentiation of BMSCs. When decellularized “book” scaffolds with BMSCs sheets were used to repair a 1 mm Achilles tendon defect, histomorphological analysis, immunohistochemical assessment, and biomechanical testing showed that the book‐shaped decellularized tendon matrix scaffold and BMSCs sheets could promote the regeneration of type I collagen at the wound site during healing, and improve the mechanical properties of the repaired tendon. Therefore, the results of this study suggest that the novel decellularized “book” tendon scaffolds combined with BMSCs sheets have therapeutic effects on improving the healing quality of the Achilles tendon. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 9999:1–11, 2019.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Book‐shaped decellularized tendon matrix scaffold combined with bone marrow mesenchymal stem cells‐sheets for repair of achilles tendon defect in rabbit

Xie

Zhou

Tang

et al. 2019

Journal Orthopaedic Research

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“…For the past two decades, biodegradable polymers have been explored as vehicles to deliver retinal cells into the subretinal space. Previous experience with synthetic scaffolds such as poly (lactic-co-glycolic acid) (PLGA) [3][4][5] , poly(glycol sebacate) (PGS) 6,7 , and polycaprolactone (PCL) 8 , as well as proteinand carbohydrate-based materials 9,10 , resulted in improved retinal progenitor cell (RPC) post-transplant survival and differentiation to mature cells compared with a single cell suspension. However, the polymer scaffold/cell composite grafts there are solid materials that can result in more extensive retinal detachment than desirable.…”

Section: Introductionmentioning

confidence: 99%

In Situ Cross-linking Hydrogel as a Vehicle for Retinal Progenitor Cell Transplantation

et al. 2019

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One of the current limitations of retinal transplantation of stem cells as well as other cell types is the dispersion of cells from the injection site (including loss of cells into the vitreous chamber) and low survival after transplantation. Gelatin-hydroxyphenyl propionic acid (Gtn-HPA) conjugate is a biodegradable polymer that can undergo covalent cross-linking in situ, allowing for injection of incorporated cells through a small caliber needle followed by gel formation in vivo. We tested the hypothesis that Gtn-HPA hydrogel supports survival and integration of retinal progenitor cells (RPCs) post-transplantation. In vitro compatibility and in vivo graft survival were assessed by mixing an equal volume of Gtn-HPA conjugate and RPC suspension and triggering enzyme-mediated gelation, using minute amounts of horseradish peroxidase and peroxide. Immunocytochemistry showed >80% survival of cells and minimal apoptosis for cells incorporated into Gtn-HPA, equivalent to controls grown on fibronectin-coated flasks. RPCs undergoing mitosis were seen within the three-dimensional Gtn-HPA hydrogel, but the percentage of Ki-67-positive cells was lower compared with the monolayer controls. For in vivo studies, gel–cell mixture or cell suspension in saline was trans-sclerally injected into the left eye of female Long Evans rats immunosuppressed with cyclosporine A. Grafts survived at the 1 week time point of the study, with Gtn-HPA-delivered grafts showing less inflammatory response demonstrated by anti-leukocyte staining. More eyes in the gel–cell mixture group showed surviving cells in the subretinal space compared with saline-delivered controls, while the number of cells surviving per graft was not significantly different between the two groups. This work demonstrates an injectable in situ cross-linking hydrogel as a potential vehicle for stem cell delivery in the retina.

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Sustained release of stromal cell–derived factor‐1 alpha from silk fibroin microfiber promotes urethral reconstruction in rabbits

Liu

Huang

Yuan

et al. 2020

J Biomedical Materials Res

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We developed a stromal cell–derived factor‐1 alpha (SDF‐1α)‐aligned silk fibroin (SF)/three‐dimensional porous bladder acellular matrix graft (3D‐BAMG) composite scaffold for long‐section ventral urethral regeneration and repair in vivo. SDF‐1α‐aligned SF microfiber/3D‐BAMG, aligned SF microfiber/3D‐BAMG, and nonaligned SF microfiber/3D‐BAMG scaffolds were prepared using electrostatic spinning and wet processing. Adipose‐derived stem cell (ADSC) and bone marrow stromal cell (BMSC) migration was assessed in the SDF‐1α‐loaded scaffolds. Sustained SDF‐1α release in vitro and vivo was analyzed using enzyme‐linked immunosorbent assay (ELISA) and western blotting, respectively. The scaffolds were used to repair a 1.5 × 1 cm2 ventral urethral defect in male rabbits in vivo. General observation and retrograde urinary tract contrast assessment were used to examine urethral lumen patency and continuity at 1 and 3 months post‐surgery. Postoperative rehabilitation was evaluated using histological detection. The composite scaffolds sustained SDF‐1α release for over 16 days in vitro. SDF‐1α‐aligned SF nanofiber promoted regeneration of urethral mucosa, submucosal smooth muscles, and microvasculature, increased cellular proliferation, and reduced collagen deposition. SDF‐1α expression was increased in reconstructed urethra at 3 months post‐surgery in SDF‐1α‐aligned SF group. SDF‐1α‐aligned SF microfiber/3D‐BAMG scaffolds may be used to repair and reconstruct long urethral defects because they accelerate urethral regeneration.

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A biodegradable scaffold enhances differentiation of embryonic stem cells into a thick sheet of retinal cells

Cited by 51 publications

References 61 publications

Book‐shaped decellularized tendon matrix scaffold combined with bone marrow mesenchymal stem cells‐sheets for repair of achilles tendon defect in rabbit

Book‐shaped decellularized tendon matrix scaffold combined with bone marrow mesenchymal stem cells‐sheets for repair of achilles tendon defect in rabbit

In Situ Cross-linking Hydrogel as a Vehicle for Retinal Progenitor Cell Transplantation

Sustained release of stromal cell–derived factor‐1 alpha from silk fibroin microfiber promotes urethral reconstruction in rabbits

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A biodegradable scaffold enhances differentiation of embryonic stem cells into a thick sheet of retinal cells

Cited by 51 publications

References 61 publications

­Book‐shaped decellularized tendon matrix scaffold combined with bone marrow mesenchymal stem cells‐sheets for repair of achilles tendon defect in rabbit

­Book‐shaped decellularized tendon matrix scaffold combined with bone marrow mesenchymal stem cells‐sheets for repair of achilles tendon defect in rabbit

In Situ Cross-linking Hydrogel as a Vehicle for Retinal Progenitor Cell Transplantation

Sustained release of stromal cell–derived factor‐1 alpha from silk fibroin microfiber promotes urethral reconstruction in rabbits

Contact Info

Product

Resources

About

Book‐shaped decellularized tendon matrix scaffold combined with bone marrow mesenchymal stem cells‐sheets for repair of achilles tendon defect in rabbit

Book‐shaped decellularized tendon matrix scaffold combined with bone marrow mesenchymal stem cells‐sheets for repair of achilles tendon defect in rabbit