A Bioengineered Human Skin Tissue for the Treatment of Infected Wounds

Thomas-Virnig, Christina L.; Allen-Hoffmann, B. Lynn

doi:10.1089/wound.2011.0338

Cited by 10 publications

(9 citation statements)

References 36 publications

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“…Ultimately, wound closure with autologous epithelium restores both a source of innate immunity, such as defensins and cathelicidins 55,56 in the epidermis, and a full complement of immune effector cells in the dermal tissue. Together with improved nutrition to maintain the cellular immunity 57,58 , accomplishment of wound closure with autologous epidermis remains a definitive factor to long-term control of microbial contamination and infection of burn wounds.…”

Section: 0 State Of the Sciencementioning

confidence: 99%

“…Since 2006, advanced models of engineered skin substitutes have been described that consist of allogeneic cells to provide temporary protection of wounds, and to promote epithelial closure with autologous epidermal keratinocytes in the wound, or dermal-epidermal autografts 69,70 . An initial model of gene therapy with an engineered skin model secretes elevated levels of the native antimicrobial peptide, cathelicidin, and has been cleared for clinical trial 56 . Engineered skin substitutes with autologous keratinocytes are capable of providing sufficient epidermal cell populations to cover burns up to 99% TBSA, and may be applied as keratinocyte sheets, sprays of cell suspensions, or bi-layered compositions with both epidermal keratinocytes and dermal fibroblasts 71 .…”

Section: 0 State Of the Sciencementioning

confidence: 99%

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Burn Wound Healing and Tissue Engineering

Singer

Boyce

2017

Journal of Burn Care & Research

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Section: 0 State Of the Sciencementioning

confidence: 99%

Section: 0 State Of the Sciencementioning

confidence: 99%

Burn Wound Healing and Tissue Engineering

Singer

Boyce

2017

Journal of Burn Care & Research

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“…All of these have the potential to alter the biocompatibility of the material. Skin systems have properties that are different if they are to serve as a graft versus a functional replacement [22,23,24,25,26,27,28,29]. In this review, the emphasis will be on scaffold systems that intend to get the properties at least to the functional replacement level and as close as possible to the native state.…”

Section: Introductionmentioning

confidence: 99%

“…Effort has even gone into making channels that will become the new blood vessels [7]. Again as 3D bioprinting has improved, more effort has been toward making the structures more biomimetic (morphologically and chemically) to serve more like a graft substitute versus a degradable/regenerative scaffold [4,22,23,24,25,26,27,28,29].…”

Section: Introductionmentioning

confidence: 99%

Biomaterial Enhanced Regeneration Design Research for Skin and Load Bearing Applications

Feldman

2019

JFB

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Biomaterial enhanced regeneration (BER) falls mostly under the broad heading of Tissue Engineering: the use of materials (synthetic and natural) usually in conjunction with cells (both native and genetically modified as well as stem cells) and/or biological response modifiers (growth factors and cytokines as well as other stimuli, which alter cellular activity). Although the emphasis is on the biomaterial as a scaffold it is also the use of additive bioactivity to enhance the healing and regenerative properties of the scaffold. Enhancing regeneration is both moving more toward regeneration but also speeding up the process. The review covers principles of design for BER as well as strategies to select the best designs. This is first general design principles, followed by types of design options, and then specific strategies for applications in skin and load bearing applications. The last section, surveys current clinical practice (for skin and load bearing applications) including limitations of these approaches. This is followed by future directions with an attempt to prioritize strategies. Although the review is geared toward design optimization, prioritization also includes the commercializability of the devices. This means a device must meet both the clinical performance design constraints as well as the commercializability design constraints.

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“…Of these, 100,000 people are hospitalized at almost $75,000 per admission, while nearly 4000 die [2,3,4]. In recent years, better infection control, better surgical techniques, and development of skin substitutes have reduced the death rate by over half from 30 years ago [4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22]. Now, the critical needs are to not only get better in these three areas, but also to have better assessment techniques, faster wound closure times, and to improve the quality of the new tissues formed [23].…”

Section: Introductionmentioning

confidence: 99%

Fibrin as a Tissue Adhesive and Scaffold with an Angiogenic Agent (FGF-1) to Enhance Burn Graft Healing In Vivo and Clinically

Feldman

Osborne

2018

JFB

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There is a need for a strategy to reduce scarring in meshed skin graft healing leading to a better cosmetic result without a significant increase in cost. The strategy in this paper is to increase the closure rate of a meshed skin graft to reduce scarring, which should also decrease the infection rate. Specifically, we used fibrin glue to attach all parts of the graft to the wound bed and added in an angiogenic growth factor and made the fibrin porous to further help the growth of blood vessels from the wound bed into the graft. There was a 10-day animal study and a one-month clinical study. Neither making the fibrin porous or adding an angiogenic agent (i.e., fibroblast growth factor-1 (FGF-1)) seemed to make a significant improvement in vivo or clinically. The use of fibrin on a meshed skin graft appears to speed up the regenerative healing rate leading to less scarring in the holes in the mesh. It appears to shorten the healing time by five days and keep the tissue stiffness close to normal levels vs. the doubling of the stiffness by the controls. A larger clinical study, however, is needed to definitively prove this benefit as well as the mechanism for this improvement.

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A Bioengineered Human Skin Tissue for the Treatment of Infected Wounds

Cited by 10 publications

References 36 publications

Burn Wound Healing and Tissue Engineering

Burn Wound Healing and Tissue Engineering

Biomaterial Enhanced Regeneration Design Research for Skin and Load Bearing Applications

Fibrin as a Tissue Adhesive and Scaffold with an Angiogenic Agent (FGF-1) to Enhance Burn Graft Healing In Vivo and Clinically

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