A bioinformatic insight into the genetic diversity within pomegranate cultivars: from nuclear to chloroplast genes

Shahsavari, Shiva; Noormohammadi, Zahra; Sheidai, Masoud; Farahani, Farah; Vazifeshenas, Mohammad Reza

doi:10.1007/s10722-021-01297-z

Cited by 3 publications

(1 citation statement)

References 16 publications

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“…A developing of SSRs was introduced as a rapid tool for the identification of pomegranate cultivars in the study of nutraceutical and genetic diversity of novel pomegranate genotypes in comparison to leading commercial pomegranate varieties (Arlotta et al, 2022 ). In addition, the genetic variability in pomegranate cultivars was reported using both nuclear (SSRs) and chloroplast genetic regions ( trnH‐psbA spacer, and matk gene; Shahsavari et al, 2022 ). However, diversity in flower characteristics has received less attention.…”

Section: Introductionmentioning

confidence: 99%

Morphological diversity of indigenous wild pomegranate (Punica granatum L. var. spinosa) accessions from northeast of Iran

Ashrafi

Bodaghi

Rezaei

2022

Food Science & Nutrition

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Wild pomegranate is a valuable edible species in the plant ecosystem of the Hyrcanian forests and the northern plains of the Caspian coast in Iran. The genetic diversity of these wild pomegranates can be effective in pomegranate breeding programs and germplasm conservation. In the present study, morphological diversity in 103 wild pomegranates ( Punica granatum L . var. spinosa ) in the northeastern area of Iran was studied using 46 traits related to trees, flowers, and fruits. The results showed that the fruit weight ranged from 17.93 to 99.9 g with an average of 48.92 g, the total aril weight ranged from 0.54 g to 64.78 g with an average of 24.25 g, and the weight of 100 arils was between 4.89 and 46.21 with an average of 13.79. The fruit cracking percent, crown shape, aril juiciness, calyx, and corolla colors show a high coefficient of variation (CV > 70%). Based on PCA results, fruit weight and total aril weight, peel weight, and fruit length and diameter were important on determining differences among accessions. In biplot analysis, genotype distribution was determined by two main factors. In cluster analysis, the studied accessions were divided into two different major clusters and two subclusters in each one. The results showed a high diversity of important pomological traits in wild pomegranates such as fruit weight, fruit cracking percent, crown shape, total aril weight, aril juiciness corolla, and calyx color that can be used in breeding programs to improve pomegranate juice quality and marketability.

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Section: Introductionmentioning

confidence: 99%

Morphological diversity of indigenous wild pomegranate (Punica granatum L. var. spinosa) accessions from northeast of Iran

Ashrafi

Bodaghi

Rezaei

2022

Food Science & Nutrition

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Employing plant DNA barcodes for pomegranate species identification in Al-Baha Region, Saudi Arabia

Omari Alzahrani,

Dguimi,

Alshaharni

et al. 2023

J.Umm Al-Qura Univ. Appll. Sci.

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The Punica granatum (Pomegranate) tree attracted a lot of interest for its nutritious fruits and therapeutic benefits. Although research on genetic diversity is important to develop breeding programs and implement efficient cultivar improvement, the genetic diversity of pomegranates in Saudi Arabia has not been investigated completely. The two important pomegranate cultivars in Al-Baha region of Saudi Arabia (Bidah-red and Bidah-green), which have recently gained considerable attention due to their unique sweet taste, were studied using DNA barcodes because information about their phylogeny is limited. To reveal the phylogeny of these two cultivars, three DNA barcodes [the internal transcribed spacer 2 (ITS2), ribulose 1,5-biphosphate carboxylase (rbcL), and intergenic spacer region (trnH-psbA)] were used. The ITS2 and psbA-trnH had sufficient polymorphism to allow distinction at the cultivar level, whereas the rbcL region had a uniform sequence; hence, it failed to discriminate among the cultivars. The two cultivars were found to be clustered in the same clade on the phylogenetic tree constructed using the ITS2 and psbA-trnH sequences, suggesting that they are either closely related or have adapted to their locations. As the ITS2 region exhibited higher polymorphism than psbA-trnH, the phylogenetic tree based on ITS2 indicated that Bidah-red and Bidah-green are distinct cultivars. We conclude that ITS2 and psbA-trnH DNA barcodes are capable of authenticating and identifying pomegranate cultivars and can assist in improving pomegranate quality in the future through molecular breeding.

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Molecular Characterization and Cuticalar Stomatal Anatomy of Punica Granatum L. Cultivars Study in Dohuk Governorate

Sinjare,

Abdulrahman,

Khalid

et al. 2024

Preprint

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The pomegranate (Punica granatum L.) has a rich history of cultivation and is renowned for its beneficial properties and nutrient-dense fruits. Native to Central Asia, particularly Iran, Punica granatum has spread to various parts of the world, including Mediterranean countries, Russia, Japan, India, Afghanistan, Pakistan, California, and China. The pomegranate has garnered increasing global interest due to its high nutritional value, as well as its ornamental, industrial, and medicinal benefits. Identifying molecular markers that are less affected by environmental conditions is crucial for measuring genetic diversity among pomegranate cultivars and assisting breeders in selecting and developing genotypes with higher quality and consistency. This study aimed to explore the genetic diversity and stomatal characteristics of nine pomegranate cultivars collected from various locations in Duhok Governorate, Kurdistan Region/Iraq. Genetic diversity was assessed using a combination of molecular methods, including Start Codon Targeted (SCoT) markers, SRAP markers, and the Internal Transcribed Spacer (ITS) gene region, trnG-trnS gene, and 18S–28S rDNA intergenic spacer. The ITS region yielded a product of 700 bp, the trnG-trnS gene was 580 bp, and the 18S–28S rDNA intergenic spacer was 854 bp. Digestion of the trnG-trnS gene with the restriction enzyme HaeIII produced a single amplicon of approximately 490 bp across all samples. The results demonstrated that both SCoT and SRAP markers were effective for analyzing genetic diversity in pomegranates. Additionally, the stomatal structure analysis revealed significant variability among cultivars, with consistent internal structures observed across the cultivars. However, measurements of stomatal components varied depending on the growing location. This study highlights the wide genetic diversity among pomegranate cultivars and the impact of environmental factors on stomatal characteristics.

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A bioinformatic insight into the genetic diversity within pomegranate cultivars: from nuclear to chloroplast genes

Cited by 3 publications

References 16 publications

Morphological diversity of indigenous wild pomegranate (Punica granatum L. var. spinosa) accessions from northeast of Iran

Morphological diversity of indigenous wild pomegranate (Punica granatum L. var. spinosa) accessions from northeast of Iran

Employing plant DNA barcodes for pomegranate species identification in Al-Baha Region, Saudi Arabia

Molecular Characterization and Cuticalar Stomatal Anatomy of Punica Granatum L. Cultivars Study in Dohuk Governorate

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