A Bioinspired Injectable, Adhesive, and Self‐Healing Hydrogel with Dual Hybrid Network for Neural Regeneration after Spinal Cord Injury

Abstract

Hydrogel‐based regenerated scaffold shows promise as a platform for neural regeneration following spinal cord injury (SCI). Nevertheless, the persistent problem of poor mechanical strength and limited integration with the host tissue still exists. In this study, we developed a bioinspired hydrogel with highly sophisticated features for neural regeneration after SCI. The hydrogel is composed of DOPA‐grafted chitosan and a designer peptide, offering a unique set of qualities such as being injectable, having self‐healing abilities, and adhering to tissues. Compared to the conventional hydrogel, our hydrogel insured as significant promotion of the immune response modulation and axon regrowth while featuring synapse formation of various neurotransmitters and myelin regeneration. Subsequently, functional recoveries were enhanced, including motor function, sensory function and particularly bladder defect repair. These positive findings demonstrate that the hydrogel has great potential as a strategy for repairing SCI. Moreover, the versatility of this strategy goes beyond neural regeneration and holds promise for tissue regeneration in other contexts. Overall, our proposed hydrogel represented an innovative and multifaceted tool for engineering structures in the biomedical field.This article is protected by copyright. All rights reserved

In Situ Reaction-Generated Aldehyde-Scavenging Polypeptides-Curcumin Conjugate Nanoassemblies for Combined Treatment of Spinal Cord Injury

“…Biomaterials that can manipulate the phenotypes of immune cells have proved effective in improving treatment outcome of SCI. 44 Since CUR could regulate the polarization of microglia, 28 the anti- inflammatory effect of PFCN was investigated by analyzing the M1/M2 polarization of microglia. BV2 cells were pretreated with lipopolysaccharide (LPS) to establish an inflammation model in vitro.…”

“…It has been reported that activated microglia after SCI could polarize to two distinct subsets, i.e., cytotoxic pro-inflammatory (M1) phenotype and pro-repair anti-inflammatory (M2) phenotype. 44 The M1 microglia plays an important role in the mediation of inflammation after SCI, whereas M2 microglia represents immunosuppression, upregulation of extracellular matrix components, and tissue repair. Biomaterials that can manipulate the phenotypes of immune cells have proved effective in improving treatment outcome of SCI.…”

Polysaccharide-Based Double-Network Hydrogels: Polysaccharide Effect, Strengthening Mechanisms, and Applications

“…CHT is a polycationic copolymer formed by the N -deacetylation of chitin and comprises glucosamine and N -acetylglucosamine units . It is widely exploited on account of its cytocompatibility, antibacterial and hemostatic activities, biodegradability, and muco-adhesiveness. ,, Furthermore, CHT dissolves in an acidic solution (such as acetic acid) and is insoluble under neutral and alkaline conditions. CHT is soluble at a pH lower than ∼6.3 and in ferric solutions through coordination interaction among ferric ions, hydroxyl groups, and amino groups .…”

“…138 It is widely exploited on account of its cytocompatibility, antibacterial and hemostatic activities, biodegradability, and muco-adhesiveness. 80,138,139 Furthermore, CHT dissolves in an acidic solution (such as acetic acid) and is insoluble under neutral and alkaline conditions.…”

Recent Advancements of Bioinks for 3D Bioprinting of Human Tissues and Organs

“…However, to ensure their neuroregenerative function, further in vivo transplantation experiments are necessary. Furthermore, based on hydrogel-based bioinks, the regenerative scaffolds exhibit a wide range of prospects as a novel platform for treating nervous system-related diseases such as spinal cord injury, drug delivery, neural regeneration, and repair . For the fabricated constructs used in transplantation, the ultimate goal is to reconstruct the cellular microenvironment that can process various signals and cues, providing guidance for cell behavior and function.…”