A bioluminescent probe for in vivo imaging of pyroglutamate aminopeptidase in a mouse model of inflammation

Hu, Shilong; Chen, Xinxin; Qin, Xiaojun; Dong, Gaopan; Lin, Yuxing; Gai, Wenrui; Zhao, Hanqing; Ke, Bowen; Li, Minyong

doi:10.1016/j.bmcl.2021.128049

Cited by 9 publications

(8 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[109,110] Hu et al used the firefly luciferase system to develop a probe PBL that constructed by coupling D-aminofluorescein with pyroglutamate via an amide bond, which can be considered as a dipeptide derivative and can be used to detect PGP in vitro and in vivo (Figure 6). [111] In vivo imaging of a mouse model of symptomatic liver disease demonstrated excellent sensitivity and selectivity of the probe. The probe provided a powerful tool for studying the physiological and pathological processes of PGP, which allowed the detection of PGP activity in living animals of various pathological conditions and normal physiological processes including inflammation.…”

Section: Detection Of Inflammationmentioning

confidence: 98%

See 2 more Smart Citations

A Review on Recent Advances in Peptide‐based Fluorescence Probes and their Potential Applications

Wang,

Chen

et al. 2023

ChemistrySelect

View full text Add to dashboard Cite

With the development and maturity of classical solid‐state synthesis techniques, peptide synthesis has become more convenient. With the continuous progress of research in the field of life sciences, more and more peptides have been elucidated in terms of their structure and biological functions. Due to the excellent biocompatibility, specificity, selectivity, and low cytotoxicity, peptide‐based fluorescent probes have been widely explored and applied in chemical, biological, medical and other fields. This review summarized the characteristics of peptide‐based fluorescence probes in terms of long fluorescence lifetime, high stability, large Stokes shift, high fluorescence quantum yield, high selectivity and sensitivity. Several types of peptide‐based fluorescence probes, such as peptide basic fluorescence, embedded fluorescence molecules, coupled fluorescence groups, and self‐assembled fluorescence, were also described. Finally, we summarized the applications of peptide‐based fluorescent probes in biological imaging, disease detection, ion detection, and biomolecule detection. This review may provide a guarantee and inspiration for further development of the peptide‐based fluorescent probes in the field of biological applications.

show abstract

Section: Detection Of Inflammationmentioning

confidence: 98%

Section: Detection Of Inflammationmentioning

confidence: 99%

See 1 more Smart Citation

A Review on Recent Advances in Peptide‐based Fluorescence Probes and their Potential Applications

Wang,

Chen

et al. 2023

ChemistrySelect

View full text Add to dashboard Cite

show abstract

“…22 Moreover, the intrinsic drawbacks of fluorescence imaging, including photobleaching, autofluorescence, and light scattering, restrict its applicability as a method for in vivo biological detection and imaging. 23,24 Therefore, it is still necessary to develop more efficient and novel strategies for the in vitro and in vivo monitoring of CES2.…”

Section: Introductionmentioning

confidence: 99%

Identification of the first selective bioluminescent probe for real-time monitoring of carboxylesterase 2 in vitro and in vivo

Chen,

Zhao,

Miao

et al. 2024

Analyst

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition to fluorescence, bioluminescence generated by luciferase-catalyzed oxidation of chemical substrates is an alternative for analytes detection in complex samples. , In comparison with fluorescence probes, the bioluminescent probes cause negligible background autofluorescence due to the elimination of external optical excitation, providing high signal-to-noise ratio and sensitivity in detection. − Previous bioluminescent probes usually cage the bioluminescent substrates, such as luciferin and coelenterazine, with specific recognition units to form a prosubstrate, which can be oxidized by luciferase after reaction with targets. , Based on this strategy, many bioluminescent probes have achieved gratifying results in in vitro visual detection, bioimaging of diseases biomarkers, such as enzymes, − metal ions, , reactive oxygen species (ROS), and reactive sulfide species (RSS). − However, the signal intensity of the turn-on bioluminescent probes decays sharply with the consumption of substrate, which severely hinders their quantitative analysis in practical applications.…”

Section: Introductionmentioning

confidence: 99%

A Reaction-Based Ratiometric Bioluminescent Platform for Point-of-Care and Quantitative Detection Using a Smartphone

Wang

Xiong

et al. 2023

Anal. Chem.

View full text Add to dashboard Cite

Fluorescent probes have emerged as powerful tools for the detection of different analytes by virtue of structural tenability. However, the requirement of an excitation source largely hinders their applicability in point-of-care detection, as well as causing autofluorescence interference in complex samples. Herein, based on bioluminescence resonance energy transfer (BRET), we developed a reaction-based ratiometric bioluminescent platform, which allows the excitation-free detection of analytes. The platform has a modular design consisting of a NanoLuc-HaloTag fusion as an energy donor, to which a synthetic fluorescent probe is bioorthogonally labeled as recognition moiety and energy acceptor. Once activated by the target, the fluorescent probe can be excited by NanoLuc to generate a remarkable BRET signal, resulting in obvious color changes of luminescence, which can be easily recorded and quantitatively analyzed by a smartphone. As a proof of concept, a fluorescent probe for HOCl was synthesized to construct the bioluminescent system. Results demonstrated the system showed a constant blue/red emission ratio which is independent to the signal intensity, allowing the quantification of HOCl concentration with high sensitivity (limit of detection (LOD) = 13 nM) and accuracy. Given the universality, this reaction-based bioluminescent platform holds great potential for point-of-care and quantitative detection of reactive species.

show abstract

A bioluminescent probe for in vivo imaging of pyroglutamate aminopeptidase in a mouse model of inflammation

Cited by 9 publications

References 30 publications

A Review on Recent Advances in Peptide‐based Fluorescence Probes and their Potential Applications

A Review on Recent Advances in Peptide‐based Fluorescence Probes and their Potential Applications

Identification of the first selective bioluminescent probe for real-time monitoring of carboxylesterase 2 in vitro and in vivo

A Reaction-Based Ratiometric Bioluminescent Platform for Point-of-Care and Quantitative Detection Using a Smartphone

Contact Info

Product

Resources

About