A biomaterial approach to cell reprogramming and differentiation

Long, Joseph T.; Kim, Hye-Jin; Kim, Dajeong; Lee, Jong Bum; Kim, Deok Ho

doi:10.1039/c6tb03130g

Cited by 27 publications

(19 citation statements)

References 111 publications

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“…On the other hand, PEGDA hydrogels without RGD modifications or with caged RGD support few adherent cells and the cells have round morphology. Besides, it has been reported that RGD peptides can be introduced to polymers, such as HA (Lee et al, 2015 ), alginate (Sun et al, 2017 ), chitosan (Kim et al, 2016 ), and PEG (Long et al, 2017 ), by chemical binding to fabricated bioactive hydrogels. These functionalized hydrogels show improved biological properties to promote cell adhesion, spreading, proliferation, and differentiation.…”

Section: Control Of Chemical Propertiesmentioning

confidence: 99%

Functional Hydrogels With Tunable Structures and Properties for Tissue Engineering Applications

et al. 2018

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Tissue engineering (TE) has been used as an attractive and efficient process to restore the original tissue structures and functions through the combination of biodegradable scaffolds, seeded cells, and biological factors. As a unique type of scaffolds, hydrogels have been frequently used for TE because of their similar 3D structures to the native extracellular matrix (ECM), as well as their tunable biochemical and biophysical properties to control cell functions such as cell adhesion, migration, proliferation, and differentiation. Various types of hydrogels have been prepared from naturally derived biomaterials, synthetic polymers, or their combination, showing their promise in TE. This review summarizes the very recent progress of hydrogels used for TE applications. The strategies for tuning biophysical and biochemical properties, and structures of hydrogels are first introduced. Their influences on cell functions and promotive effects on tissue regeneration are then highlighted.

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Section: Control Of Chemical Propertiesmentioning

confidence: 99%

Functional Hydrogels With Tunable Structures and Properties for Tissue Engineering Applications

et al. 2018

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“…The technique was subsequently refined and developed by several research groups (e.g., [45,46,47,48,49]). Although avoiding the problem of IM, the efficiency of piPSC protocols is generally low (0.001–0.006%) [50], this being most likely due to failure of the proteins to penetrate the hydrophobic cell membrane [51]. The low cell permeability has been partly overcome through fusion of the proteins with cell penetrating peptides [44] or, more recently, by treatment of the donor cells with titanium oxide nanotubes [52], and use of bolaamphiphiles to stabilize cell membrane proteins and so facilitate uptake of the proteins [53].…”

Section: Reprogramming Leads To Genetic Dysregulationmentioning

confidence: 99%

iPS-Cell Technology and the Problem of Genetic Instability—Can It Ever Be Safe for Clinical Use?

Attwood

Edel

2019

JCM

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The use of induced Pluripotent Stem Cells (iPSC) as a source of autologous tissues shows great promise in regenerative medicine. Nevertheless, several major challenges remain to be addressed before iPSC-derived cells can be used in therapy, and experience of their clinical use is extremely limited. In this review, the factors affecting the safe translation of iPSC to the clinic are considered, together with an account of efforts being made to overcome these issues. The review draws upon experiences with pluripotent stem-cell therapeutics, including clinical trials involving human embryonic stem cells and the widely transplanted mesenchymal stem cells. The discussion covers concerns relating to: (i) the reprogramming process; (ii) the detection and removal of incompletely differentiated and pluripotent cells from the resulting medicinal products; and (iii) genomic and epigenetic changes, and the evolutionary and selective processes occurring during culture expansion, associated with production of iPSC-therapeutics. In addition, (iv) methods for the practical culture-at-scale and standardization required for routine clinical use are considered. Finally, (v) the potential of iPSC in the treatment of human disease is evaluated in the light of what is known about the reprogramming process, the behavior of cells in culture, and the performance of iPSC in pre-clinical studies.

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“…Hydrogels of hyaluronic acid give further examples of the extraordinary influence on ESC and PSC proliferation and differentiation Poly-L-lactic acid (PLLA)/poly-glycolic acid (PLGA), Matrigel, each possesses the capacity to choreograph cartilage, endothelial tubules, neurons, and hepatocytes. [27][28][29] Innovative solutions to the problem focus on the spatial and temporal separation of the defining factors in cell transformations. [30] Precisely targeting the individual cell with transcription factors, functional gene sequences, small proteins, and chemically defined small molecules is a prominent role for dynamic, information-rich biological materials and their structures.…”

Section: Introductionmentioning

confidence: 99%

“…Hydrogels of hyaluronic acid give further examples of the extraordinary influence on ESC and PSC proliferation and differentiation Poly‐L‐lactic acid (PLLA)/poly‐glycolic acid (PLGA), Matrigel, each possesses the capacity to choreograph cartilage, endothelial tubules, neurons, and hepatocytes. [ 27–29 ]…”

Section: Introductionmentioning

confidence: 99%

Sequenced Somatic Cell Reprogramming and Differentiation Inside Nested Hydrogel Droplets

Green

Watson

et al. 2020

Adv. Biosys.

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from the patient. The cells would then be safer and not cause immune responses. The most promising cells for human cell and tissue therapy are Human embryonic stem cells (hESC's), human pluripotent cells (hPC's), and embryonic-like human induced pluripotent stem cells (hiPSC's). This is because they indefinitely divide into new stem cells, and they can transform into many other phenotypes, given the right conditions. [1,2] Using healthy patient's cells from skin or fat tissues requires hard technical skills in transforming cell phenotypes between different and disparate specialized phenotypes. The best practice is to create pluripotent cell phenotypes. Clinical success is guaranteed when the cells originate from the patient. Effective cell therapy requires technical skills in transforming cell identities between different and disparate specialties. The best practice is to create pluripotent cell phenotypes that can generate very large populations. [3-5] PSC's possess an inordinate capacity to generate any of the approximately 200 different specialized cell types. This is achieved through the fine-scale modulation of biochemical, physical, [6-8] mechanical, and material permutations. [2] These highly potent PSCs provide the best possible among any cell type to generate multiple billions of cells for every patient. [3] Simple, fast, scalable and highly efficient methods of cell reprogramming and differentiation into billions of stable therapeutic cells are essential for sizeable tissue substitutions or largescale physiological restoration in regenerative medicine. [3-5] However, reprogramming protocols are the use of integrating or nonintegrating vectors. [9,10] Invariably, the current methods, which are based predominantly on gene integration, frequently use viral vectors. This, despite their perfectly adapted ability to lead to low transformation efficiencies typically between 0.01 and 1% and a maximum of up to 7%. [11-13] The production of PSCs with extraordinary rates of efficiency, leading to increased yields is vital to generate clinically relevant tissue volumes. There are reports of reprogramming rates of 30%. Replanting the freshly reprogrammed cells on laminin coated surfaces increases the level pluripotency to 80% within a fibroblast population. That is after a spell of continuous culture for 14 days on VTN-coated substrates. On this theme, the treatment of fibroblasts with recombinant proteins coded by Yamanaka transcription factors dramatically increased reprogramming to The efficient genesis of pluripotent cells or therapeutic cells for regenerative medicine involves several external manipulations and conditioning protocols, which drives down clinical applicability. Automated programming of the genesis by microscale physical forces and chronological biochemistry can increase clinical success. The design and fabrication of nested polysaccharide droplets (millimeter-sized) with cell sustaining properties of natural tissues and intrinsic properties for time and space evolution of cell transformation sign...

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A biomaterial approach to cell reprogramming and differentiation

Cited by 27 publications

References 111 publications

Functional Hydrogels With Tunable Structures and Properties for Tissue Engineering Applications

Functional Hydrogels With Tunable Structures and Properties for Tissue Engineering Applications

iPS-Cell Technology and the Problem of Genetic Instability—Can It Ever Be Safe for Clinical Use?

Sequenced Somatic Cell Reprogramming and Differentiation Inside Nested Hydrogel Droplets

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