A biomimetic renal fibrosis progression model on-chip evaluates anti-fibrotic effects longitudinally in a dynamic fibrogenic niche

Abstract

Although renal fibrosis can advance chronic kidney disease and progressively lead to end-stage renal failure, no effective anti-fibrotic drugs have been clinically approved. To aid drug development, we developed a...

DLM–GelMA/tumor slice sandwich structured tumor on a chip for drug efficacy testing

“…The decellularized liver scaffold was prepared as previously described. 22,23,29 Sprague-Dawley rats and their livers were used in this study with the approval of the Animal Ethics Committee of West China Hospital of Sichuan University (no. 20221205004).…”

“…Decellularized tissues retained their biophysical morphology, contained ample ECM, biochemical factors and proteins, and were widely used as tissue implants for biomedical purposes. 22–24 However, the structural integrity and stability of tissue constructs based on a decellularized matrix are notably constrained by its low mechanical stability, degradation and deformation. 25–27 It was noted that the incorporation of a decellularized matrix with GelMA significantly enhanced the mechanical properties.…”

Fibrosis in Chronic Kidney Disease: Pathophysiology and Therapeutic Targets

“…The protein encourages apoptosis and prevents the proliferation of vascular endothelial cells [103]. Disrupting or inhibiting key players involved in fibroti niche formation are already being explored for future CKD therapy [104].…”