A biorefinery approach to microbial oil production from glycerol by Rhodotorula glutinis

Karamerou, Eleni E.; Theodoropoulos, Constantinos; Webb, Colin

doi:10.1016/j.biombioe.2016.01.007

Cited by 41 publications

(31 citation statements)

References 32 publications

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“…250 g/L) . Moreover, the conversion of glycerol into SCO has been seen in R. toruloides or other red strains principally the last years . The reported L max value ( c .…”

Section: Discussionmentioning

confidence: 96%

Production of secondary metabolites through glycerol fermentation under carbon‐excess conditions by the yeasts Yarrowia lipolytica and Rhodosporidium toruloides

Papanikolaou

Kampisopoulou

Blanchard

et al. 2017

Euro J Lipid Sci & Tech

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The biochemical behavior of the yeasts Rhodosporidium toruloides DSM 4444, Yarrowia lipolytica ACA YC 5029, and Y. lipolytica ACA YC 5030 was evaluated on waste glycerol‐based media, added at increasing initial concentrations (50–140 g/L), with nitrogen remaining constant. During flask cultivation of Y. lipolytica ACA‐YC 5030, and although medium pH remained >4.8, major metabolites were the polyols mannitol (CMl) and erythritol (CEr) (CMlmax = 32.1 g/L, conversion yield per glycerol consumed =0.23 g/g, and CErmax = 35.5 g/L, yield ≈0.25 g/g). For the strain ACA‐YC 5029, equally in flasks and pH >4.8, the major metabolites were again mannitol and erythritol, with CMlmax = 28.9 g/L (conversion yield ≈0.21 g/g), and CErmax = 33.6 g/L (yield ≈0.24 g/g). In contrast, in batch‐bioreactor experiment, the yeast ACA YC‐5029 produced mainly citric acid (maximum concentration =39 g/L, yield on glycerol =0.42 g/g). R. toruloides DSM 4444 was flask‐cultured at increasing initial glycerol quantities (up to ≈140 g/L) and displayed significant growth producing also satisfactory amounts of lipids. The maximum DCW concentration achieved was 39.1 g/L. Maximum lipids were 13.7 g/L (at that point lipid in DCW was 37.0% w/w). The major cellular fatty acid produced was oleic acid. Phospholipids of R. toruloides were the most unsaturated fraction among lipid fractions quantified. Practical applications: Industrial glycerol, the main by‐product of biodiesel‐producing facilities, appears in constantly increasing quantities in the market volume due to the worldwide rise of biodiesel production, rendering the valorization of this by‐product as a very important scientific priority. In this report, the yeasts Y. lipolytica ACA YC 5029 and Y. lipolytica ACA YC 5030 were cultivated in submerged batch experiments with industrial glycerol as substrate under nitrogen limitation and appreciable quantities of mannitol and erythritol, compounds presenting significant importance for the food industries, were synthesized. The yeast R. toruloides DSM 4444, equally batch‐cultured on glycerol under nitrogen limitation, produced appreciable quantities of biomass that contained high concentrations of microbial lipids, that could be transformed into “2nd generation” biodiesel. The current study, hence, provides viable possibilities of glycerol valorization through its use as substrate in order metabolic compounds of added‐value to be synthesized. Crude glycerol, the principal “waste” stream deriving from biodiesel production process, is converted with the aid of microbial fermentations into secondary metabolites useful for the food‐ and biofuels‐producing industries. The metabolic compounds synthesized are microbial lipids (single cell oils) amenable to be converted into 2nd generation biodiesel with fermentations led by a strain of the yeast Rhodosporidium toruloides and mannitol, erythritol and citric acid with fermentations led by strains of the yeast Yarrowia lipolytica.

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“…250 g/L) . Moreover, the conversion of glycerol into SCO has been seen in R. toruloides or other red strains principally the last years . The reported L max value ( c .…”

Section: Discussionmentioning

confidence: 96%

Production of secondary metabolites through glycerol fermentation under carbon‐excess conditions by the yeasts Yarrowia lipolytica and Rhodosporidium toruloides

Papanikolaou

Kampisopoulou

Blanchard

et al. 2017

Euro J Lipid Sci & Tech

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“…T. elegans 14Á9 3 4 Á5 Flasks Zikou et al (2013) interesting alternative to the use of plant oils for biodiesel production because oleaginous yeasts, due to their unicellular morphology can be cultivated more easily than the fungi in large-scale bioreactors, whereas strains of these yeasts can transform a variety of substrates including low-or even zero-cost materials to SCO, which can then be transformed to biodiesel (Xue et al 2006;Li et al 2007;Zhao et al 2008;Li et al 2010;Xu et al 2012Xu et al , 2013Xu et al , 2016Xu et al , 2017Tsakona et al 2014Tsakona et al , 2016Karamerou et al 2015;Louhasakul and Cheirsilp, 2013;Tchakouteu et al 2015aTchakouteu et al , 2017Karamerou et al 2017). It is evident that as far as the synthesis of SCO that is aimed to be transformed in second-generation biodiesel is concerned, only hydrophilic carbon sources (i.e.…”

Section: Oleaginous Micro-organismsmentioning

confidence: 99%

Lipids from yeasts and fungi: physiology, production and analytical considerations

et al. 2018

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The last years there has been a significant rise in the number of publications in the international literature that deal with the production of lipids by microbial sources (the 'single cell oils; SCOs' that are produced by the so-called 'oleaginous' micro-organisms). In the first part of the present review article, a general overview of the oleaginous micro-organisms (mostly yeasts, algae and fungi) and their potential upon the production of SCOs is presented. Thereafter, physiological and kinetic events related with the production of, mostly, yeast and fungal lipids when sugars and related substrates like polysaccharides, glycerol, etc. (the de novo lipid accumulation process) or hydrophobic substrates like oils and fats (the ex novo lipid accumulation process) were employed as microbial carbon sources, are presented and critically discussed. Considerations related with the degradation of storage lipid that had been previously accumulated inside the cells, are also presented. The interplay of the synthesis of yeast and fungal lipids with other intracellular (i.e. endopolysaccharides) or extracellular (i.e. citric acid) secondary metabolites synthesized is also presented. Finally, aspects related with the lipid extraction and lipidome analysis of the oleaginous micro-organisms are presented and critically discussed.

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“…This allowed for the development of a two-stage cultivation strategy where the first stage maximized biomass and carotenoid production under irradiation/high temperature and then maximized lipid content when switched to dark/low temperature [63]. Additional growth studies have evaluated the effect of pH with potato wastewater and glycerol [65], modulating air flow rates using glycerol with yeast extract as the nutrient supply without pH control [66] and using lignocellulosic biomass [67]. Other parameters for scale-up were explored using an airlift bioreactor with mixed carbon sources [68].…”

Section: Rhodotorula Glutinismentioning

confidence: 99%

The Oleaginous Red YeastRhodotorula/Rhodosporidium: A Factory for Industrial Bioproducts

Lyman¹,

Urbin²,

Strout³

et al. 2019

Yeasts in Biotechnology

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Rhodotorula genus, amended in 2015, is polyphyletic and contains Rhodotorula species that grow as single-cell yeast (monomorphic) and reproduce asexually via budding/fission (anamorphic); it also contains Rhodosporidium species that reproduce sexually (teleomorphic) and alternate between a yeast phase and dikaryotic filamentous phase (dimorphic). Several species of these "red yeast" produce industrial bioproducts, namely biofuel feedstocks, carotenoids, enzymes, and biosurfactants. This chapter highlights the biotechnology areas that Rhodotorula/ Rhodosporidium contributes to and the future market value of those industries. The primary yeast species to be discussed include Rhodosporidium toruloides, Rhodotorula glutinis, Rhodosporidium diobovatum, Rhodosporidium kratochvilovae, Rhodotorula graminis, Rhodotorula babjevae, and Rhodotorula taiwanensis.

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A biorefinery approach to microbial oil production from glycerol by Rhodotorula glutinis

Cited by 41 publications

References 32 publications

Production of secondary metabolites through glycerol fermentation under carbon‐excess conditions by the yeasts Yarrowia lipolytica and Rhodosporidium toruloides

Production of secondary metabolites through glycerol fermentation under carbon‐excess conditions by the yeasts Yarrowia lipolytica and Rhodosporidium toruloides

Lipids from yeasts and fungi: physiology, production and analytical considerations

The Oleaginous Red YeastRhodotorula/Rhodosporidium: A Factory for Industrial Bioproducts

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