A biosensor approach to probe the structure and function of the adsorbed proteins: fibrinogen at the gold surface

Snopok, B. A.; Kostyukevych, K. V.; Rengevych, O.V.; Shirshov, Yu.M.; Venger, É. F.; Kolesnikova, I. N.; Lugovskoi, E. V.

doi:10.15407/spqeo1.01.121

Cited by 33 publications

(22 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly to collagen, its molecular weight is about 340 kilodaltons. Fibrinogen adsorbed on various surfaces has been imaged by AFM as well [46], down to molecular resolution [47,48,49,50].…”

Section: Introductionmentioning

confidence: 99%

Combined surface acoustic wave and surface plasmon resonance measurement of collagen and fibrinogen layer physical properties

Friedt

Francis

2016

Sensing and Bio-Sensing Research

View full text Add to dashboard Cite

We use an instrument combining optical (surface plasmon resonance) and acoustic (Love mode surface acoustic wave device) real-time measurements on a same surface for the identification of water content in collagen and fibrinogen protein layers. After calibration of the surface acoustic wave device sensitivity by copper electrodeposition and surfactant adsorption, the bound mass and its physical properties -density and optical index -are extracted from the complementary measurement techniques and lead to thickness and water ratio values compatible with the observed signal shifts. Such results are especially usefully for protein layers with a high water content as shown here for collagen on an hydrophobic surface. We obtain the following results: collagen layers include 70±20% water and are 16±3 to 19±3 nm thick for bulk concentrations ranging from 30 to 300 µg/ml. Fibrinogen layers include 50±10% water for layer thicknesses in the 6±1.5 to 13±2 nm range when the bulk concentration is in the 46 to 460 µg/ml range.

show abstract

Section: Introductionmentioning

confidence: 99%

Combined surface acoustic wave and surface plasmon resonance measurement of collagen and fibrinogen layer physical properties

Friedt

Francis

2016

Sensing and Bio-Sensing Research

View full text Add to dashboard Cite

show abstract

“…The structural peculiarities of surface topography are especially important for promising set of optoelectronic sensors. Among them particular interest is directed towards development of those sensors which are based on evanescent wave phenomena [7], in particular surface plasmon resonance (SPR) [8]. The surface structure of thin film of noble metals (i.e.…”

mentioning

confidence: 99%

Interfacial architecture on the fractal support: polycrystalline gold films as support for self-assembling monolayers

Snopok¹,

Strizhak²,

Kostyukevich³

et al. 1999

SPQEO

View full text Add to dashboard Cite

Specific chemical functionality (i.e. molecular recognition, energy transfer, chemical conversions, etc.) may be only realized within a definitive length scale of structured matter [1]. Particularly, a certain level of functional complexity is a result of combining smaller subunits that are spatially arranged in a specific manner. Regulation of spatial complexity allows one to realize desired functional goal of a material. This rather broad concept encompasses a wide variety of issues of current technological demand [2]. For instance, sensing optical devices play a central role in optoelectronic intelligent massifs realizing the principal of «chemical imaging» [3]. Further development of these intelligent devices requires an integration of two-dimensional molecular recognition interfaces and transducers transformation of the primarily recognition response into a signal of chemical sensor [4]. Chemical processes at interfaces are governed by three main factors: molecular functionality, distribution of active units, and geometry of the surface [5]. Whereas relative more tremendous knowledge have been accumulated related to the first, and, to a certain extent to the second factor, a little is known regarding a role of surface irregularities for the

show abstract

“…One can see that the SPR angle increases with STI adsorption onto the initial gold surface (Fig. 3) are flattens out, giving rise to an SPR angle shift of ca.1450±100′′ within a period of about 1000 s. For adsorbed proteins their surface concentration can be calculated from the SPR data in accordance with [35]. If one takes for the refractive index increment the average value of 0.188 cm 3 g -1 [42] for both STI and trypsin, then the adsorbed amount of STI on the initial gold surface gives experimental value of about 1.5 ng/mm 2 .…”

Section: Interaction Between Trypsin and Adsorbed Stimentioning

confidence: 81%

“…Analysis of kinetic curves was made in accordance with the classical Langmuir kinetic theory. For direct analysis of SPR data the following expressions were used [35,36]:…”

Section: Methodsmentioning

confidence: 99%

Biochemical passivation of metal surfaces for sensor application: reactive annealing of polycrystalline gold films in hydrogen sulfide atmosphere

Snopok¹

2000

Semicond. Phys. Quantum Electron. Optoelectron.

Self Cite

View full text Add to dashboard Cite

The formation of Au x S y interfacial layer by reactive annealing of gold films in H 2 S atmosphere is investigated. This seems to be a technologically favorable technique for the large-scale and low-cost fabrication of nondestructive immobilization support for biological molecules. Formation of phases with different chemical functionality and surface topography as a function of reaction time was studied using Atomic Force Microscopy (AFM), Surface Plasmon Resonance (SPR) measurements and biomolecular interaction analysis (trypsin-Soybean Trypsin Inhibitor (STI) reaction). The results obtained confirm the classical two-step model for the sulfide phase formation during reactive annealing. This includes an intermediate formation of a dispersed phase of sulfur followed by its reconstruction to a close-packed sulfide layer. Adsorption of proteins onto a certain sulfide layer passes with retention of a native state of adsorbed molecules. The proposed strategy for formation of biochemical structures (gold/sulfide/proteins) on the surface of physical transducers opens a new way for design and development of novel artificial smart sensing systems. They not only maintain optimal functioning of bioreceptors but also are responsive to their environment.

show abstract

A biosensor approach to probe the structure and function of the adsorbed proteins: fibrinogen at the gold surface

Cited by 33 publications

References 40 publications

Combined surface acoustic wave and surface plasmon resonance measurement of collagen and fibrinogen layer physical properties

Combined surface acoustic wave and surface plasmon resonance measurement of collagen and fibrinogen layer physical properties

Interfacial architecture on the fractal support: polycrystalline gold films as support for self-assembling monolayers

Biochemical passivation of metal surfaces for sensor application: reactive annealing of polycrystalline gold films in hydrogen sulfide atmosphere

Contact Info

Product

Resources

About