A biotechnological process efficiently co-produces two high value-added products, glucose and xylooligosaccharides, from sugarcane bagasse

Xue, Jian-Long; Zhao, Shuai; Liu, Ruiming; Yin, Xin; Jiang, Sui-Xin; Su, Lin‐Hui; Yang, Qi; Duan, Chengjie; Liu, Junliang; Feng, Jia‐Xun

doi:10.1016/j.biortech.2015.12.082

Cited by 64 publications

(17 citation statements)

References 32 publications

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“…oxalicum strain HP7-1 and its cellulase and xylanase hyper-producing mutant EU2106. This microorganism has potential for application in the bio-refinery of lignocellulosic biomass [ 18 , 27 , 28 ]. Unsurprisingly, there were no mutations in the catalytic residues of the major cellulase and hemicellulase enzymes in EU2016.…”

Section: Discussionmentioning

confidence: 99%

Comparative genomic, transcriptomic and secretomic profiling of Penicillium oxalicum HP7-1 and its cellulase and xylanase hyper-producing mutant EU2106, and identification of two novel regulatory genes of cellulase and xylanase gene expression

Zhao

Yan

et al. 2016

Biotechnol Biofuels

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125

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BackgroundThe filamentous fungus Penicillium oxalicum is a potential alternative to Trichoderma reesei for industrial production of a complete cellulolytic enzyme system for a bio-refinery. Comparative omics approaches can support rational genetic engineering and/or breeding of filamentous fungi with improved cellulase production capacity. In this study, comparative genomic, transcriptomic and secretomic profiling of P. oxalicum HP7-1 and its cellulase and xylanase hyper-producing mutant EU2106 were employed to screen for novel regulators of cellulase and xylanase gene expression.ResultsThe 30.62 Mb P. oxalicum HP7-1 genome was sequenced, and 9834 protein-coding genes were annotated. Re-sequencing of the mutant EU2106 genome identified 274 single nucleotide variations and 12 insertion/deletions. Comparative genomic, transcriptomic and secretomic profiling of HP7-1 and EU2106 revealed four candidate regulators of cellulase and xylanase gene expression. Deletion of these candidate genes and measurement of the enzymatic activity of the resultant mutants confirmed the identity of three regulatory genes. POX02484 and POX08522, encoding a putative Zn(II)2Cys6 DNA-binding domain and forkhead protein, respectively, were found to be novel, while PoxClrB is an ortholog of ClrB, a key transcriptional regulator of cellulolytic enzyme gene expression in filamentous fungi. ΔPOX02484 and ΔPOX08522 mutants exhibited significantly reduced β-glucosidase activity, increased carboxymethylcellulose cellulase and xylanase activities, and altered transcription level of cellulase and xylanase genes compared with the parent strain ΔPoxKu70, with Avicel as the sole carbon source.ConclusionsTwo novel genes, POX02484 and POX08522, were found and characterized to regulate the expression of cellulase and xylanase genes in P. oxalicum. These findings are important for engineering filamentous fungi to improve cellulase and xylanase production.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-016-0616-9) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Comparative genomic, transcriptomic and secretomic profiling of Penicillium oxalicum HP7-1 and its cellulase and xylanase hyper-producing mutant EU2106, and identification of two novel regulatory genes of cellulase and xylanase gene expression

Zhao

Yan

et al. 2016

Biotechnol Biofuels

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125

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“…We performed a study to facilitate the production of XOS in the same culture of enzyme production, with the effect to lower cultivation time and of production costs, using the rice husk, which has not been pretreated, contrasting with most of other studies, in which residues are physicochemically pretreated (Jayapal et al ., ; Azelee et al ., ; Xue et al ., ).…”

Section: Resultsmentioning

confidence: 97%

“…Xylooligosaccharides used as functional food depending on the degree of polymerisation may be fermented by these bacteria and selectively stimulate the growth or activity of one or a limited number of these probiotic bacteria, which is known as the prebiotic effect (Gibson, 2007;Vandenplas et al, 2011). We performed a study to facilitate the production of XOS in the same culture of enzyme production, with the effect to lower cultivation time and of production costs, using the rice husk, which has not been pretreated, contrasting with most of other studies, in which residues are physicochemically pretreated (Jayapal et al, 2013;Azelee et al, 2016;Xue et al, 2016).…”

Section: Production Of Xylooligosaccharidesmentioning

confidence: 99%

Comparative production of xylanase and the liberation of xylooligosaccharides from lignocellulosic biomass by Aspergillus brasiliensis BLf1 and recombinant Aspergillus nidulans XynC A773

Menezes

Rossi

Squina

et al. 2018

Int J of Food Sci Tech

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Summary We investigated the simultaneous production of xylanase and the liberation of xylooligosaccharides in rice husk solid‐state cultivations of Aspergillus brasiliensis BLf1 and by the recombinant Aspergillus nidulans XynC A773 strain. The bioprocess was optimised by experimental fractional design and response surface analysis. Results show that both fungi strains produced xylanases and their activities were dependent on the addition of basal medium, moisture content and the interactions between particle size and inoculum size, producing maximum xylanase activities of 230.7 U g−1 substrate for A. brasiliensis, and 187.9 U g−1 substrate for A. nidulans XynC. Xylooligosaccharides were liberated in the same cultures, with concentrations up to 17.6 mg g−1 and 23.9 mg g−1 of substrate for A. brasiliensis and A. nidulans XynC, respectively, both strains presenting similar profiles, with xylose residues varying from X3 to X6. These results suggest the possibility of lowering production costs of enzymes for food applications and oligosaccharides.

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“…A productivity of 2.36, 2.76, 2.03, and 2.17 mg/mL of X2, X3, X4, and X5, respectively, was obtained after hydrolysis of sugarcane bagasse by Streptomyces rameus L2001 xylanase [133]. A maximum yield of 5.96% was obtained for the conversion of sugarcane bagasse being xylobiose and xylotriose the main products [134]). …”

Section: Xylanasesmentioning

confidence: 90%

Microbial Glycosidases for Nondigestible Oligosaccharides Production

Bezerra¹,

Montibra

Hansen

et al. 2017

Enzyme Inhibitors and Activators

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There is much interest in the study and production of nondigestible oligosaccharides (NDOs), due to their bioactivities and beneficial effects to the human health. The main approach in the production of NDOs relies on the action of glycosidases performing hydrolysis or transglycosylation of polysaccharides and sugars. In this chapter, a description of the main microbial glycosidases used for NDOs production, their sources, their principal properties, and a description of the production processes with the better results obtained are discussed.

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A biotechnological process efficiently co-produces two high value-added products, glucose and xylooligosaccharides, from sugarcane bagasse

Cited by 64 publications

References 32 publications

Comparative genomic, transcriptomic and secretomic profiling of Penicillium oxalicum HP7-1 and its cellulase and xylanase hyper-producing mutant EU2106, and identification of two novel regulatory genes of cellulase and xylanase gene expression

Comparative genomic, transcriptomic and secretomic profiling of Penicillium oxalicum HP7-1 and its cellulase and xylanase hyper-producing mutant EU2106, and identification of two novel regulatory genes of cellulase and xylanase gene expression

Comparative production of xylanase and the liberation of xylooligosaccharides from lignocellulosic biomass by Aspergillus brasiliensis BLf1 and recombinant Aspergillus nidulans XynC A773

Microbial Glycosidases for Nondigestible Oligosaccharides Production

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