A Biphasic Excitatory Response of Hippocampal Neurons to Gonadotropin-Releasing Hormone

Palovcik, Reinhard A.; Phillips, M. Ian

doi:10.1159/000124636

Cited by 32 publications

(12 citation statements)

References 13 publications

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“…Electrophysiological study showed that hippocampal neurons had biphasic excitatory response to GnRH (Lu et al 1999;Palovcik and Phillips 1986). LH can also regulate the function of hippocampal neuron (Lei and Rao 2001).…”

Section: Discussionmentioning

confidence: 99%

A study on co-localization of FSH and its receptor in rat hippocampus

2007

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It has been known that GnRH, LH and their receptors exist in hippocampal neurons. However, whether FSH and its receptor also exist in hippocampal neurons remained unknown yet. In situ hybridization, double-labeled immunofluorescence stain and double-labeled immunohistochemistry stain in adjacent sections were used in our research to study the distribution, co-localization of FSH and its receptor and co-localization of FSH and GnRH receptor in rat hippocampus. The result found that pyramidal neurons from CA1 to CA4 region and granule neurons in dentate gyrus could express FSH and its receptor, majority of hippocampal neurons co-expressed FSH and its receptor, FSH and GnRH receptor. These suggested that hippocampal neurons not only express FSH but also act as FSH target cells. FSH may regulate the function of hippocampal neurons by ways of paracrine or autocrine. At the same time, GnRH may regulate the function of FSH neuron in hippocampus through GnRH receptor.

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Section: Discussionmentioning

confidence: 99%

A study on co-localization of FSH and its receptor in rat hippocampus

2007

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“…On the other hand, destruction of hippocampal structures enhances the expression of sexual behavior [32], That GnRH could be involved in that process is indicated by electrophysiological experiments. Firing rates recorded from slices in vitro are increased in a large population of hippocampal neurons upon application of GnRH [33].…”

Section: Discussionmentioning

confidence: 99%

Characterization and Distribution of Receptors for Gonadotropin-Releasing Hormone in the Rat Hippocampus

et al. 1988

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Distribution and properties of receptors for gonadotropin-releasing hormone (GnRH) were analyzed in the brain of adult male rats. Binding of the iodinated GnRH agonist Des-Gly¹⁰-(D-Ala⁶)-GnRH ethylamide was studied in hippocampus and anterior pituitary using three convergent approaches: quantitative autoradiography on frozen tissue, binding to fresh slices, and binding to crude membrane preparations. In all cases, binding was specific, saturable, and time, pH, and temperature dependent. Quantitative autoradiography revealed that the density of binding sites was high in the stratum oriens and stratum radiatum of the CA1-CA4 regions of Ammon’s horn. The pyramidal cell layer was faintly labelled. Binding was almost undetectable in the dentate gyrus. The highest density of sites (B_max = 11.6 + 1.0 fmol/mg protein) was observed in the stratum radiatum of the CA3 region. Under the same conditions the value obtained for pituitary tissues was 20.7 + 2.8 fmol/mg protein. Analysis of saturation curves indicated only one class of high-affinity sites for the hippocampus (CA₃; K_d = 0.28 + 0.03 nM) and for the pituitary (K_d = 0.29 ± 0.08 nM). Both native GnRH and GnRH antagonist were potent competitors of binding. Fresh slices and membrane preparations from whole hippocampus confirmed these autoradiographic data and yielded affinity constants of 0.28 + 0.01 and 0.52 + 0.08 nM, respectively. In addition, a very high binding density was present in the amygdaloid complex, while binding was barely detectable in the hypothalamus. These results demonstrate that high densities of specific GnRH receptors are present in areas concerned with the regulation of behavioral functions. GnRH may thus be involved as a neurotransmitter in the integration of endocrine and behavioral reproductive processes.

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“…Slices were kept in aCSF on ice until required, and previous studies showed that no significant sample degradation occurred during this time (up to 7 h) (16, 17). Slices have been perfused for 30-40 h while maintaining stable spontaneous action potentials, indicating slice viability (16,17).…”

Section: Methodsmentioning

confidence: 99%

MR microscopy of perfused brain slices

Blackband

Bui

Buckley

et al. 1997

Magnetic Resonance in Med

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To study the origins of signal changes in clinical MRI we have previously studied isolated single neuronal cells by MR microscopy. To account for the extracellular environment of the cells, we have developed a prototype perfusion chamber for MR microimaging of perfused rat hippocampal brain slices. To demonstrate the utility of this model, brain slices were initially perfused in isotonic solutions and then subjected to osmotic perturbations via perfusate exchange with 20% hypertonic and 20% hypotonic solutions. In diffusion weighted images, signal intensity changes of +16(sigma(n-1) = 11)% (hypotonic) and -26(sigma(n-1) = 10)% (hypertonic) were observed. No significant variation in response was observed across the slice when several subregions were examined. These observations are consistent with the view that contrast changes are driven primarily by changes in the intra- and extracellular compartmentation of water. This is the first report of MR microimaging of the isolated brain slice. The technique will enable the correlation of MR microimaging measurements with microscopic changes using other modalities and techniques to provide a better understanding of signals in clinical MRI.

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A Biphasic Excitatory Response of Hippocampal Neurons to Gonadotropin-Releasing Hormone

Cited by 32 publications

References 13 publications

A study on co-localization of FSH and its receptor in rat hippocampus

A study on co-localization of FSH and its receptor in rat hippocampus

Characterization and Distribution of Receptors for Gonadotropin-Releasing Hormone in the Rat Hippocampus

MR microscopy of perfused brain slices

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