A bleaching herbicidal activity of methoxyhygromycin (MHM) produced by an actinomycete strainStreptomycessp. 8E-12

Abstract

Aims: To screen bioherbicidal isolates and evaluate herbicidal activity of methoxyhygromycin (MHM) produced by Streptomyces sp. 8E-12. Methods and Results: Streptomyces sp. 8E-12 with herbicidal activity was selected through seed germination bioassay. An active metabolite, MHM was isolated from culture broth by carbon absorption, butanol extraction, silica gel, Sephadex LH-20 and G-10 chromatography, and preparative HPLC. The metabolite was identified by electrospray ionization mass spectra (ESI-MS) and 1 H-and 13 C-NMR spectral data analyses. In vivo herbicidal activity was examined against weeds and crops grown on pots. Conclusions: Streptomyces sp. 8E-12 produced a selective herbicidal metabolite which was identified as MHM. The metabolite showed stronger in vivo activity against monocotyledonous plants than dicotyledonous plants, and caused a bleaching (albino symptom) on some weeds including Digitaria sanguinalis and Echinochloa crus-galli. Significance and Impact of the Study: These results showed that Streptomyces sp. 8E-12 produced a bioherbicidal metabolite, MHM and can be developed as a biocontrol agent (BCA) for weed control.

Evaluation of a Fungal Strain, Myrothecium roridum F0252, as a Bioherbicide Agent

“…A fourth evaluation of in vivo herbicidal activity of M. roridum F0252 against 15 plants was performed both in pre-and post-plant emergence by a whole plant spray method (Lee et al, 2003). Bioherbicidal activity of the ground culture broth containing conidia and mycelial fragments and an ethyl acetate (EtOAc) extract of the broth were tested on pots (surface area×height=350 cm 2 ×8 cm) containing 15 plants using a hand sprayer, according to the method by Lee et al (2003).…”

“…A fourth evaluation of in vivo herbicidal activity of M. roridum F0252 against 15 plants was performed both in pre-and post-plant emergence by a whole plant spray method (Lee et al, 2003). Bioherbicidal activity of the ground culture broth containing conidia and mycelial fragments and an ethyl acetate (EtOAc) extract of the broth were tested on pots (surface area×height=350 cm 2 ×8 cm) containing 15 plants using a hand sprayer, according to the method by Lee et al (2003). The 13 weedy plants tested against were velvetleaf (Abutilon avicennae), Indian jointvetch (Aeschynomene indica), cheatgrass (Agropyron smithii), bindweed (Calystegia japonica), large crabgrass (Digitaria sanguinalis), barnyardgrass (Echinochloa crus-galli), monochoria (Monochoria vaginalis), fall panicum (Panicum dichotomiflorum), arrowhead (Sagittaria pygmaea), bulrush (Scirpus juncoides), black nightshade (Solanum nigrum), clover (Trifolium repens), and cocklebur (Xanthium strumarium), and the two crops represented were rice (Oryza sativa) and sorghum (Sorghum bicolor).…”

Distinct tRNA Accommodation Intermediates Observed on the Ribosome with the Antibiotics Hygromycin A and A201A

“…HygA also displays strong in vitro potency against Serpulina (Treponema) hyodysenteriae (Nakagawa et al, 1987; Omura et al, 1987), the causative agent of swine dysentery, an economically significant muco-hemorrhagic disease of pigs. In addition, HygA and its derivatives have been reported to have herbicidal (Kim et al, 1990; Lee et al, 2003) as well as immunosuppressant properties (Uyeda et al, 2001; Yoshida et al, 1986). Biochemical studies indicate that HygA inhibits translation by binding to the peptidyl transferase center (PTC) on the large ribosomal subunit and preventing the binding of aminoacyl-tRNA to the A-site (Guerrero and Modolell, 1980; Polacek et al, 2002; Poulsen et al, 2000).…”

Herbicidal Activity of Herbicidin from a Strain of Soil Actinomycete Streptomyces scopuliridis

“…국내의 경우 현재까지 미생물, 자생식물, 식물의 정유성 분 등으로부터 천연 제초활성물질 탐색을 위한 연구가 수행 되어왔으나, 아직까지는 상업화는 이루어지지 않고 있다. 대 표적으로 Streptomyces 속에서 유래한 methoxyhygromycin (MHM)은 제초활성 물질로서의 가능성이 있다고 보고되었 으나, 높은 처리농도 등의 문제로 실용화되지 못하였다 (Lee et al, 2003).…”