A bloody evidence: Is<i>Mycobacterium bovis</i>bacteraemia frequent in cattle?!

Maggioli, Mayara F.

doi:10.1080/21505594.2016.1213477

Cited by 5 publications

(3 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TST false-negative cattle presented exacerbated gross lesions with a predominant multi-organ commitment ( 5 ), which can occur because of haematogenous dissemination of the bacilli from the primary infection area. This fact suggests that viable mycobacteria could be detectable in blood samples, as previously confirmed in naturally infected cattle ( 11 ). Regarding M. bovis detection in blood, previous studies developed different specific PCRs in blood, targeting M. bovis sequences ( mpb70 , esxB gene, RD4 region, IS 6110 ), with variable results ( 12–16 ).…”

Section: Introductionsupporting

confidence: 76%

Limited usefulness of the IS6110 touchdown-PCR in blood for tuberculin skin test false-negative cattle with serological response to Mycobacterium bovis

Encinas,

Ferrara Muñiz,

Sammarruco

et al. 2024

Front. Vet. Sci.

View full text Add to dashboard Cite

Ante-mortem diagnosis of bovine tuberculosis (bTB) is based mainly on the tuberculin skin test (TST) and the ɣ-IFN release assay (IGRA). Some infected animals escape screening tests, thus, limit herd sanitation. Previous reports have suggested a predominant pattern of multi-organ lesions attributable to Mycobacterium bovis (the causative agent of bTB) bacteraemia. A case–control study was conducted to investigate blood PCR as an alternative tool for improving ante-mortem detection of TST false-negative bovines. Cases comprised 70 TST false-negative bovines (cases), which were serology positive, and controls included 81 TST positive bovines; all of them confirmed as infected with M. bovis. Detection of the IS6110 target through touchdown blood-PCR (IS6110 TD-PCR) was performed. The positivity of the blood-PCR was 27.2% in the control group. This performance was similar to the 15% obtained among cases (p = 0.134). Most cases identified by the IS6110 TD-PCR exhibited focalized lesions (p = 0.002). Results demonstrated that blood-PCR could detect TST false-negative cattle, even if they are negative for IGRA. Considering that cases exhibited humoral response to M. bovis, further studies conducted in a pre-serological stage could provide evidence about the real contribution of the technique in herds.

show abstract

Section: Introductionsupporting

confidence: 76%

Limited usefulness of the IS6110 touchdown-PCR in blood for tuberculin skin test false-negative cattle with serological response to Mycobacterium bovis

Encinas,

Ferrara Muñiz,

Sammarruco

et al. 2024

Front. Vet. Sci.

View full text Add to dashboard Cite

show abstract

“…However, further studies about bacteremia and the time of dissemination of M. bovis in blood stream is needed to detect the proper sampling time because most of TST and IGRA reactor animals failed to be detected by PCR in blood samples [ 4 ]. Despite the high risk of disseminated infection of M. bovis , bacteremia has been assumed to be rare in cattle [ 115 ].…”

Section: Diagnosis Of Btbmentioning

confidence: 99%

Review on Bovine Tuberculosis: An Emerging Disease Associated with Multidrug-Resistant Mycobacterium Species

Borham¹,

Oreiby

El-Gedawy³

et al. 2022

Pathogens

View full text Add to dashboard Cite

Bovine tuberculosis is a serious infectious disease affecting a wide range of domesticated and wild animals, representing a worldwide economic and public health burden. The disease is caused by Mycobacteriumbovis and infrequently by other pathogenic mycobacteria. The problem of bovine tuberculosis is complicated when the infection is associated with multidrug and extensively drug resistant M. bovis. Many techniques are used for early diagnosis of bovine tuberculosis, either being antemortem or postmortem, each with its diagnostic merits as well as limitations. Antemortem techniques depend either on cellular or on humoral immune responses, while postmortem diagnosis depends on adequate visual inspection, palpation, and subsequent diagnostic procedures such as bacterial isolation, characteristic histopathology, and PCR to reach the final diagnosis. Recently, sequencing and bioinformatics tools have gained increasing importance for the diagnosis of bovine tuberculosis, including, but not limited to typing, detection of mutations, phylogenetic analysis, molecular epidemiology, and interactions occurring within the causative mycobacteria. Consequently, the current review includes consideration of bovine tuberculosis as a disease, conventional and recent diagnostic methods, and the emergence of MDR-Mycobacterium species.

show abstract

“…The identification of disseminated infections and bacteremia caused by M. bovis in cattle remains a challenge due to the unreliability of commonly used diagnostic tools [ 87 ]. The study by Swift et al [ 49 ] is so far the only available report on the use of conventional PA for rapid detection of MT complex bacteremia in animals.…”

Section: Phage Amplification Assay For Detection Of Mycobacteria Smentioning

confidence: 99%

Phage Amplification Assay for Detection of Mycobacterial Infection: A Review

Beinhauerová

Slaná

2021

Microorganisms

View full text Add to dashboard Cite

An important prerequisite for the effective control, timely diagnosis, and successful treatment of mycobacterial infections in both humans and animals is a rapid, specific, and sensitive detection technique. Culture is still considered the gold standard in the detection of viable mycobacteria; however, mycobacteria are extremely fastidious and slow-growing microorganisms, and therefore cultivation requires a very long incubation period to obtain results. Polymerase Chain Reaction (PCR) methods are also frequently used in the diagnosis of mycobacterial infections, providing faster and more accurate results, but are unable to distinguish between a viable and non-viable microorganism, which results in an inability to determine the success of tuberculosis patient treatment or to differentiate between an active and passive infection of animals. One suitable technique that overcomes these shortcomings mentioned is the phage amplification assay (PA). PA specifically detects viable mycobacteria present in a sample within 48 h using a lytic bacteriophage isolated from the environment. Nowadays, an alternative approach to PA, a commercial kit called Actiphage™, is also employed, providing the result within 6–8 h. In this approach, the bacteriophage is used to lyse mycobacterial cells present in the sample, and the released DNA is subsequently detected by PCR. The objective of this review is to summarize information based on the PA used for detection of mycobacteria significant in both human and veterinary medicine from various kinds of matrices.

show abstract

A bloody evidence: IsMycobacterium bovisbacteraemia frequent in cattle?!

Cited by 5 publications

References 11 publications

Limited usefulness of the IS6110 touchdown-PCR in blood for tuberculin skin test false-negative cattle with serological response to Mycobacterium bovis

Limited usefulness of the IS6110 touchdown-PCR in blood for tuberculin skin test false-negative cattle with serological response to Mycobacterium bovis

Review on Bovine Tuberculosis: An Emerging Disease Associated with Multidrug-Resistant Mycobacterium Species

Phage Amplification Assay for Detection of Mycobacterial Infection: A Review

Contact Info

Product

Resources

About