A BODIPY‐Based Donor/Donor–Acceptor System: Towards Highly Efficient Long‐Wavelength‐Excitable Near‐IR Polymer Dots with Narrow and Strong Absorption Features

Chen, Lei; Chen, Dandan; Jiang, Yifei; Zhang, Jicheng; Yu, Jian; DuFort, Christopher C.; Hingorani, Sunil R.; Zhang, Xuanjun; Wu, Changfeng; Chiu, Daniel T.

doi:10.1002/ange.201902077

Cited by 5 publications

(2 citation statements)

References 54 publications

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“…Upconversion nanoparticles are developed based on their deep tissue penetration and weak background interference, yet complex mechanisms associated with a nonlinear photon upconversion impede quantification by means of fluorescence intensity. Semiconducting polymer dots (Pdots) have received considerable attention in biology and medicine. − Various Pdots have been demonstrated in applications such as optical imaging, photoacoustic imaging, biosensing, phototherapy, and drug delivery. − Pdots exhibit stable fluorescence intensity in a physiological environment comprising biologically relevant ions and ROS . The Pdots also remain highly dispersed without a sign of aggregation in a biological medium.…”

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Measuring Cellular Uptake of Polymer Dots for Quantitative Imaging and Photodynamic Therapy

et al. 2021

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There is a great deal of interest in the development of nanoparticles for biomedicine. The question of how many nanoparticles are taken up by cells is important for biomedical applications. Here, we describe a fluorescence method for the quantitative measurement of the cellular uptake of polymer dots (Pdots) and a further estimation of intracellular Pdots photosensitizer for fluorescence imaging and photodynamic therapy. The approach relies on the high brightness, excellent stability, minimal aggregation quenching, and metalloporphyrin doping properties of the Pdots. We correlated the single-cell fluorescence brightness obtained from fluorescence spectrometry, confocal microscopy, and flow cytometry with the number of endocytosed Pdots, which was validated by inductively coupled plasma mass spectrometry. Our results indicated that, on average, ∼1.3 million Pdots were taken up by single cells that were incubated for 4 h with arginine 8-Pdots (40 μg/mL, ∼20 nm diameter). The absolute number of endocytosed Pdots of individual cells could be estimated from confocal microscopy by comparing the single-cell brightness with the average intensity. Furthermore, we investigated the cell viability as a result of an intracellular Pdots photosensitizer, from which the half maximal inhibitory concentration was determined to be ∼7.2 × 105 Pdots per cell under the light dose of 60 J/cm2. This study provides an effective method for quantifying endocytosed Pdots, which can be extended to investigate the cellular uptake of various conjugated polymer carriers in biomedicine.

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Measuring Cellular Uptake of Polymer Dots for Quantitative Imaging and Photodynamic Therapy

et al. 2021

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“…In this process, the ultrasound accelerates the renewal of the interface reaction, which results in local excessive of S 2- and explosive growth of the QDs. Therefore, it is difficult for QDs to maintain their original structure, thereby triggering self-quenching effect caused by aggregation [27] . Comparing the XPS images of Ag: S = 12: 7 ( Fig.…”

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Ultrasonic enhancement of microdroplet-based interfacial reaction for improving the synthesis of Ag2S QDs

Zhang

Song

et al. 2023

Ultrasonics Sonochemistry

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Controlling Energy Gaps of π‐Conjugated Polymers by Multi‐Fluorinated Boron‐Fused Azobenzene Acceptors for Highly Efficient Near‐Infrared Emission

Gon

Wakabayashi

Nakamura

et al. 2021

Chemistry An Asian Journal

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We demonstrate that multi‐fluorinated boron‐fused azobenzene (BAz) complexes can work as a strong electron acceptor in electron donor‐acceptor (D‐A) type π‐conjugated polymers. Position‐dependent substitution effects were revealed, and the energy level of the lowest unoccupied molecular orbital (LUMO) was critically decreased by fluorination. As a result, the obtained polymers showed near‐infrared (NIR) emission (λPL=758–847 nm) with high absolute photoluminescence quantum yield (ΦPL=7–23%) originating from low‐lying LUMO energy levels of the BAz moieties (−3.94 to −4.25 eV). Owing to inherent solid‐state emissive properties of the BAz units, deeper NIR emission (λPL=852980 nm) was detected in film state. Clear solvent effects prove that the NIR emission is from a charge transfer state originating from a strong D‐A interaction. The effects of fluorination on the frontier orbitals are well understandable and predictable by theoretical calculation with density functional theory. This study demonstrates the effectiveness of fluorination to the BAz units for producing a strong electron‐accepting unit through fine‐tuning of energy gaps, which can be the promising strategy for designing NIR absorptive and emissive materials.

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A BODIPY‐Based Donor/Donor–Acceptor System: Towards Highly Efficient Long‐Wavelength‐Excitable Near‐IR Polymer Dots with Narrow and Strong Absorption Features

Cited by 5 publications

References 54 publications

Measuring Cellular Uptake of Polymer Dots for Quantitative Imaging and Photodynamic Therapy

Measuring Cellular Uptake of Polymer Dots for Quantitative Imaging and Photodynamic Therapy

Ultrasonic enhancement of microdroplet-based interfacial reaction for improving the synthesis of Ag2S QDs

Controlling Energy Gaps of π‐Conjugated Polymers by Multi‐Fluorinated Boron‐Fused Azobenzene Acceptors for Highly Efficient Near‐Infrared Emission

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