A BODIPY-based fluorescent sensor for the detection of Pt2+ and Pt drugs

Tang, Fung-Kit; Zhu, Jiaqian; Kong, Fred Ka-Wai; Ng, Maggie; Bian, Qingyuan; Yam, Vivian Wing‐Wah; Tse, Anfernee Kai-Wing; Tse, Yu-Chung; Leung, Ken Cham‐Fai

doi:10.1039/d0cc00027b

Cited by 43 publications

(14 citation statements)

References 50 publications

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“…Although there have been advances in the treatment of HCC patients, the worldwide recurrence and mortality rates of HCC and HCC-associated cases are very high. NDP is a broad-spectrum antitumor drug, and it may be used in the treatment of malignant tumors, such as cervical, nasopharyngeal, esophageal, and lung cancer [24][25][26][27][28]. In recent years, a number of studies have shown that the molecular mechanism of NDP in tumors, and this potentially involved multiple potential mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Antitumor Effects of Dihydromyricetin on the Proliferation, Migration and Apoptosis of Human Hepatocellular Carcinoma Hep3B Cells

Jiang

et al. 2021

Preprint

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Background: Hepatocellular carcinoma (HCC) represents a serious public health problem worldwide and has high morbidity and mortality. Dihydromyricetin (DHM) exhibits anti-tumor effect on a variety of malignancies, but its antitumor function of DHM in HCC has been unclear. The aim of this study was designed to investigate the antitumor effect of DHM on cell apoptosis, proliferation, migration and invasion of hepatoma carcinoma cells. Methods: Cultured Hep3B cells were treated with different DHM concentrations, follow by cell apoptosis, proliferation, migration and invasion were examined by CCK-8, colony formation assay, wound healing, Transwell and flow cytometry, respectively. The mRNA and protein expression of apoptosis-associated genes and Bcl-2/Caspase-9 signaling pathway were validated by RT-PCR and western blot. Results: DHM markedly suppressed proliferation, migration, invasion and facilitated apoptosis in Hep3B cells. Mechanistically, DHM significantly downregulated the Bcl-2 expression, and upregulated the mRNA and protein levels of Cleaved-Caspase 3, Cleaved- Caspase 9, Bak, Bax and Bad. Furthermore, in the nude mice tumorigenic model, DHM treatment greatly decreased the weight of the HCC tumors compared to the weights in control and NDP group. Conclusions: DHM could suppress cell proliferation, migration, invasion, and facilitated apoptosis in Hep3B cells. These findings could provide novel insights to develop potential therapeutic strategy for the clinical treatment of HCC.

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Section: Discussionmentioning

confidence: 99%

Antitumor Effects of Dihydromyricetin on the Proliferation, Migration and Apoptosis of Human Hepatocellular Carcinoma Hep3B Cells

Jiang

et al. 2021

Preprint

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“…Our group developed several rhodamine‐based fluorescent turn‐on and ratiometric probes equipped with dithiocarbamates as recognition motifs to study the intracellular activation of Pt IV carboxylate prodrug complexes [9] . Leung and co‐workers also developed a BODIPY‐based probe for fluorescence imaging of cDDP [10] . However, these probes lack organelle‐targeting abilities and cannot be applied to study cDDP accumulation in mitochondria.…”

Section: Figurementioning

confidence: 99%

A Cisplatin‐Selective Fluorescent Probe for Real‐Time Monitoring of Mitochondrial Platinum Accumulation in Living Cells

Ong

Lee

et al. 2021

Angew Chem Int Ed

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Mitochondria have emerged as important targets for cisplatin in cancer therapy. Apart from cisplatin, anticancer Pt complexes based on similar scaffolds have also been developed to target mitochondria. Yet cellular processing of cisplatin or these mitochondria‐targeting Pt analogues remained unexplored, largely due to a lack of tools capable of probing these Pt drugs within an intracellular environment. We developed the first mitochondria‐targeted fluorescent probe for real‐time monitoring of Pt accumulation in mitochondria. We applied the probe to investigate mitochondria as cellular targets for Pt drug complexes and uncovered two distinct pathways whereby these Pt complexes could be delivered to mitochondria after cell entry.

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“…Luminescent molecular sensors are one of the most prominent among currently used in situ property measurement technologies [1][2][3][4][5][6]. Fluorescent probes demonstrate both intense callback and high versatility, due to the vast range of possible fluorescence quenching mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Bodipy Based Fluorescent Materials in Cellulose Matrices: Synthesis, Spectral Properties and Vapochromic Fluorescent Recognition of Alcohols and Acetone

et al. 2021

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This paper highlights advances made using the 4-bora-3a,4a-diaza-s-indacene (BODIPY) as a fluorophore in design and application of fluorescent sensors for microenvironment polarity. Sections of the paper cover broad analysis of a range of fluorescent indicators immobilized in ethyl-and methyl cellulose matrices. The present study demonstrates that BODIPYbased fluorescent materials could be successfully utilized for ratiometric detection of ethanol and acetone in gas phase. The achieved limit of detection value equals 0.02 mg/ml for acetone and 0.08 mg/ml for ethanol, whereas obtained sensoric materials are reusable without regeneration required.

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A BODIPY-based fluorescent sensor for the detection of Pt2+ and Pt drugs

Abstract: A novel BODIPY-based fluorescent sensor PS was designed for imaging Pt2+, cisplatin and nedaplatin in aqueous medium and biological environments, providing great potential for studying the Pt-drug metabolism and the development of new platinum drugs.

Cited by 43 publications

References 50 publications

Antitumor Effects of Dihydromyricetin on the Proliferation, Migration and Apoptosis of Human Hepatocellular Carcinoma Hep3B Cells

Antitumor Effects of Dihydromyricetin on the Proliferation, Migration and Apoptosis of Human Hepatocellular Carcinoma Hep3B Cells

A Cisplatin‐Selective Fluorescent Probe for Real‐Time Monitoring of Mitochondrial Platinum Accumulation in Living Cells

Bodipy Based Fluorescent Materials in Cellulose Matrices: Synthesis, Spectral Properties and Vapochromic Fluorescent Recognition of Alcohols and Acetone

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