A bovine miRNA, bta‐miR‐154c, withstands <i>in vitro</i> human digestion but does not affect cell viability of colorectal human cell lines after transfection

Pieri, Myrtani; Theori, Elena; Dweep, Harsh; Flourentzou, Myrofora; Kalampalika, Foteini; Maniori, Maria-Arsenia; Papagregoriou, Gregory; Papaneophytou, Christos; Felekkis, Kyriacos

doi:10.1002/2211-5463.13402

Cited by 4 publications

(1 citation statement)

References 46 publications

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“…The bta-miR-154c has been recently characterized by comestible parts of beef. This xenomiRNA is able to contrast the human digestion processes being absorbed from Caco-2, SW480, and SW620 colorectal cancer cells [ 23 ]. However, the comprehension of the specific role of miR-154c in colorectal carcinoma requires further investigation [ 23 ].…”

Section: Dietary Xenomirnas In Health and Diseasementioning

confidence: 99%

Dietary Epigenetic Modulators: Unravelling the Still-Controversial Benefits of miRNAs in Nutrition and Disease

Martino,

D’Onofrio,

Balestrieri

et al. 2024

Nutrients

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In the context of nutrient-driven epigenetic alterations, food-derived miRNAs can be absorbed into the circulatory system and organs of recipients, especially humans, and potentially contribute to modulating health and diseases. Evidence suggests that food uptake, by carrying exogenous miRNAs (xenomiRNAs), regulates the individual miRNA profile, modifying the redox homeostasis and inflammatory conditions underlying pathological processes, such as type 2 diabetes mellitus, insulin resistance, metabolic syndrome, and cancer. The capacity of diet to control miRNA levels and the comprehension of the unique characteristics of dietary miRNAs in terms of gene expression regulation show important perspectives as a strategy to control disease susceptibility via epigenetic modifications and refine the clinical outcomes. However, the absorption, stability, availability, and epigenetic roles of dietary miRNAs are intriguing and currently the subject of intense debate; additionally, there is restricted knowledge of their physiological and potential side effects. Within this framework, we provided up-to-date and comprehensive knowledge on dietary miRNAs’ potential, discussing the latest advances and controversial issues related to the role of miRNAs in human health and disease as modulators of chronic syndromes.

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Section: Dietary Xenomirnas In Health and Diseasementioning

confidence: 99%

Dietary Epigenetic Modulators: Unravelling the Still-Controversial Benefits of miRNAs in Nutrition and Disease

Martino,

D’Onofrio,

Balestrieri

et al. 2024

Nutrients

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Mechanistic Development of Cancers Associated with Processed Meat Products: A Review

Bedale,

Milkowski,

Czuprynski

et al. 2023

Meat and Muscle Biology

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Epidemiological data link processed meat products to various cancers, especially colorectal cancer; however,such evidence cannot prove causation. Clear mechanistic evidence of how these foods promote carcinogenesis strengthens the case for causation. Because the complexity and heterogeneity of processed meats as a food category complicate both epidemiological and mechanistic assessments, the study of carcinogenic mechanisms associated with specific components of such foods is often undertaken. These include components that are intrinsic to meats, those that contaminate meat, and those ingredients that are added to or form in meats during processing. Consumption of processed meats also leads to endogenous production of agents, epigenetic changes, and alterations in the microbiota of the digestive tract; therefore,the potential contributions of these endogenous responses to carcinogenesis are also discussed. This review highlights data that illuminate potential mechanisms by which agents associated with processed meats (including processed poultry) could contribute to carcinogenesis. The potential for personal factors such as overall diet, cooking methods, genetic variation, and inflammation and infection status to influence these carcinogenic mechanisms is also summarized. Because the intended audience of this review includes those who may be less familiar with current general mechanisms of mutagenesis and carcinogenesis, detailed background on these topics is provided.

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Differential Digestive Stability of Food-Derived microRNAs: The Case of miR-30c-5p and miR-92a-3p in Polyfloral Honey

Abrego-Guandique,

Ilori,

Caroleo

et al. 2024

CIMB

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Dietary microRNAs (miRs) represent a new area in food science. Although they have been found in many foods, including honey, more research is needed about their stability and fate during digestion. Hence, this study aimed to analyze the digestive stability of two selected miRs in honey. We extracted miR-92a-3p and miR-30c-5p from pasteurized and unpasteurized forms of polyfloral honey using two different methods and kits: a column-based manual method and a phenol-free semi-automated magnetic-bead-based method. The latter option was used for the subsequent analysis of samples according to the INFOGEST static in vitro digestion protocol. Also, the honey samples were examined for exosome-like particles using dynamic light scattering. Although the expression levels of both miRs were significantly lower following intestinal digestion, we found a difference in the resilience of the miRs to gastrointestinal conditions, with miR-30c-5p being relatively stable compared to miR-92a-3p following digestion, regardless of the honey’s pasteurization treatment. Moreover, there was marked heterogeneity in the extracellular vesicle profile of the pasteurized sample. We identified the presence of two broadly conserved miRs in honey: miR-92a-3p and miR-30c-5p. Despite honey exhibiting high digestibility, miR-92a-3p was less resilient than miR-30c-5p, demonstrating considerable resistance under gastrointestinal conditions. Although further research is needed, the results obtained from this study may represent a starting point for utilizing honey as a source of exogenous miRNAs for preventive strategies and more “natural” treatments.

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A bovine miRNA, bta‐miR‐154c, withstands in vitro human digestion but does not affect cell viability of colorectal human cell lines after transfection

Cited by 4 publications

References 46 publications

Dietary Epigenetic Modulators: Unravelling the Still-Controversial Benefits of miRNAs in Nutrition and Disease

Dietary Epigenetic Modulators: Unravelling the Still-Controversial Benefits of miRNAs in Nutrition and Disease

Mechanistic Development of Cancers Associated with Processed Meat Products: A Review

Differential Digestive Stability of Food-Derived microRNAs: The Case of miR-30c-5p and miR-92a-3p in Polyfloral Honey

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