A Brand New START: Abscisic Acid Perception and Transduction in the Guard Cell

Joshi-Saha, Archana; Valon, Christiane; Leung, Jeffrey

doi:10.1126/scisignal.2002164

Cited by 144 publications

(95 citation statements)

References 129 publications

(189 reference statements)

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“…In response to environmental stress, the plant hormone ABA signals stomatal closure (Zeevaart, 1980;Hirayama and Shinozaki, 2007;Cho et al, 2009;Joshi-Saha et al, 2011). This signaling pathway employs H 2 O 2 as a ROS second messenger (McAinsh et al, 1996;Murata et al, 2001;Zhang et al, 2001;Kwak et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…Guard cells use an extensive signal transduction pathway to regulate the aperture of stomata, pores on the surface of leaves that facilitate gas exchange and water loss (Roelfsema and Hedrich, 2005;Joshi-Saha et al, 2011). CO 2 , a necessary reactant for photosynthesis, enters leaves through stomata, whereas water can leave through these same pores via transpiration.…”

mentioning

confidence: 99%

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Ethylene-Induced Flavonol Accumulation in Guard Cells Suppresses Reactive Oxygen Species and Moderates Stomatal Aperture

2014

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Guard cell swelling controls the aperture of stomata, pores that facilitate gas exchange and water loss from leaves. The hormone abscisic acid (ABA) has a central role in regulation of stomatal closure through synthesis of second messengers, which include reactive oxygen species (ROS). ROS accumulation must be minimized by antioxidants to keep concentrations from reaching damaging levels within the cell. Flavonols are plant metabolites that have been implicated as antioxidants; however, their antioxidant activity in planta has been debated. Flavonols accumulate in guard cells of Arabidopsis thaliana, but not surrounding pavement cells, as visualized with a flavonolspecific dye. The expression of a reporter driven by the promoter of CHALCONE SYNTHASE, a gene encoding a flavonol biosynthetic enzyme, in guard cells, but not pavement cells, suggests guard cell-specific flavonoid synthesis. Increased levels of ROS were detected using a fluorescent ROS sensor in guard cells of transparent testa4-2, which has a null mutation in CHALCONE SYNTHASE and therefore synthesizes no flavonol antioxidants. Guard cells of transparent testa4-2 show more rapid ABA-induced closure than the wild type, suggesting that flavonols may dampen the ABA-dependent ROS burst that drives stomatal closing. The levels of flavonols are positively regulated in guard cells by ethylene treatment in the wild type, but not in the ethylene-insensitive2-5 mutant. In addition, in both ethyleneoverproducing1 and ethylene-treated wild-type plants, elevated flavonols lead to decreasing ROS and slower ABA-mediated stomatal closure. These results are consistent with flavonols suppressing ROS accumulation and decreasing the rate of ABA-dependent stomatal closure, with ethylene-induced increases in guard cell flavonols modulating these responses.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Ethylene-Induced Flavonol Accumulation in Guard Cells Suppresses Reactive Oxygen Species and Moderates Stomatal Aperture

2014

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“…Abscisic acid (ABA), a key hormone in plant response to water deficit, is a potent inducer of stomatal closure (3). ABA binds to PYR/PYL/RCAR receptors, which capture protein phosphatases 2C (4), leading to activation of Snf1-related protein kinases 2 such as SnRK2.6/OST1 (5). This protein kinase, in turn, activates several types of membrane proteins involved in stomatal closure such as NADPH oxidases (6,7); the anion channels SLAC1, SLAH1, and SLAH3 (8)(9)(10); and the plasma membrane aquaporin (AQP) AtPIP2;1 (11).…”

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confidence: 99%

Aquaporins facilitate hydrogen peroxide entry into guard cells to mediate ABA- and pathogen-triggered stomatal closure

Rodrigues

Reshetnyak

Grondin

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

273

228

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Stomatal movements are crucial for the control of plant water status and protection against pathogens. Assays on epidermal peels revealed that, similar to abscisic acid (ABA), pathogen-associated molecular pattern (PAMP) flg22 requires the AtPIP2;1 aquaporin to induce stomatal closure. Flg22 also induced an increase in osmotic water permeability (P f ) of guard cell protoplasts through activation of AtPIP2;1. The use of HyPer, a genetic probe for intracellular hydrogen peroxide (H 2 O 2 ), revealed that both ABA and flg22 triggered an accumulation of H 2 O 2 in wild-type but not pip2;1 guard cells. Pretreatment of guard cells with flg22 or ABA facilitated the influx of exogenous H 2 O 2 . Brassinosteroid insensitive 1-associated receptor kinase 1 (BAK1) and open stomata 1 (OST1)/Snf1-related protein kinase 2.6 (SnRK2.6) were both necessary to flg22-induced P f and both phosphorylated AtPIP2;1 on Ser121 in vitro. Accumulation of H 2 O 2 and stomatal closure as induced by flg22 was restored in pip2;1 guard cells by a phosphomimetic form (Ser121Asp) but not by a phosphodeficient form (Ser121Ala) of AtPIP2;1. We propose a mechanism whereby phosphorylation of AtPIP2;1 Ser121 by BAK1 and/or OST1 is triggered in response to flg22 to activate its water and H 2 O 2 transport activities. This work establishes a signaling role of plasma membrane aquaporins in guard cells and potentially in other cellular context involving H 2 O 2 signaling.aquaporin | pathogen | guard cell signaling | hydrogen peroxide | stomatal movement

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“…drought-sensitive plants revealed that drought-tolerant plants accumulated less ABA when subjected to drought stress, demonstrating differential ABA homeostasis between plant genotypes . As a primary stress hormone, ABA helps to maintain plant water status by activating downstream stress-responsive genes (Hauser et al 2011;Joshi-Saha et al 2011;Fujii and Zhu 2012). Accumulating evidence indicates that ABAmediated drought tolerance is associated with the induction of both regulatory and functional gene products (Shinozaki and Yamaguchi-Shinozaki 2007).…”

Section: Introductionmentioning

confidence: 99%

Potential role of D-myo-inositol-3-phosphate synthase and 14-3-3 genes in the crosstalk between Zea mays and Rhizophagus intraradices under drought stress

Sun

Ruan

et al. 2016

Mycorrhiza

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Arbuscular mycorrhizal (AM) symbiosis is known to stimulate plant drought tolerance. However, the mechanisms underlying the synergistic responses of the symbiotic partners to drought stress are largely unknown. A split-root experiment was designed to investigate the molecular interactions between a host plant and an AM fungus (AMF) under drought stress. In the two-compartment cultivation system, an entire or only a half root system of a maize plant was inoculated with an AMF, Rhizophagus intraradices, in the presence of localized or systemic drought treatment. Plant physiological parameters including growth, water status, and phosphorus concentration, and the expression of drought tolerance-related genes in both roots and R. intraradices were recorded. Although mycorrhizal inoculation in either one or both compartments systemically decreased abscisic acid (ABA) content in the whole root system subjected to systemic or local drought stress, we observed local and/or systemic AM effects on root physiological traits and the expression of functional genes in both roots and R. intraradices. Interestingly, the simultaneous increase in the expression of plant genes encoding D-myo-inositol-3-phosphate synthase (IPS) and 14-3-3-like protein GF14 (14-3GF), which were responsible for ABA signal transduction, was found to be involved in the activation of 14-3-3 protein and aquaporins (GintAQPF1 and GintAQPF2) in R. intraradices. These findings suggest that coexpression of IPS and 14-3GF is responsible for the crosstalk between maize and R. intraradices under drought stress, and potentially induces the synergistic actions of the symbiotic partners in enhancing plant drought tolerance.

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A Brand New START: Abscisic Acid Perception and Transduction in the Guard Cell

Cited by 144 publications

References 129 publications

Ethylene-Induced Flavonol Accumulation in Guard Cells Suppresses Reactive Oxygen Species and Moderates Stomatal Aperture

Ethylene-Induced Flavonol Accumulation in Guard Cells Suppresses Reactive Oxygen Species and Moderates Stomatal Aperture

Aquaporins facilitate hydrogen peroxide entry into guard cells to mediate ABA- and pathogen-triggered stomatal closure

Potential role of D-myo-inositol-3-phosphate synthase and 14-3-3 genes in the crosstalk between Zea mays and Rhizophagus intraradices under drought stress

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