A Brassinosteroid-Insensitive Mutant in Arabidopsis thaliana Exhibits Multiple Defects in Growth and Development

Clouse, Steven D.; Langford, Mark; McMorris, Trevor C.

doi:10.1104/pp.111.3.671

Cited by 748 publications

(596 citation statements)

References 42 publications

Supporting

Mentioning

582

Contrasting

Unclassified

Order By: Relevance

“…The brassinosteroid insensitive 1 (bri1) mutant was first identified about 10 years ago [10]. Additional alleles of bri1 were isolated in subsequent screens for mutants with similar phenotypes to BR biosynthetic mutants but cannot be rescued by the application of BR [11].…”

Section: Br Is Perceived By the Bri1 Receptor Kinase On Cell Surfacementioning

confidence: 99%

The brassinosteroid signal transduction pathway

Wang

Chong

et al. 2006

Cell Res

View full text Add to dashboard Cite

Steroids function as signaling molecules in both animals and plants. While animal steroid hormones are perceived by nuclear receptor family of transcription factors, brassinosteroids (BR) in plants are perceived by a cell surface receptor kinase, BRI1. Recent studies have demonstrated that BR binding to the extracellular domain of BRI1 induces kinase activation and dimerization with another receptor kinase, BAK1. Activated BRI1 or BAK1 then regulate, possibly indirectly, the activities of BIN2 kinase and/or BSU1 phosphatase, which directly regulate the phosphorylation status and nuclear accumulation of two homologous transcription factors, BZR1 and BES1. BZR1 and BES1 directly bind to promoters of BR responsive genes to regulate their expression. The BR signaling pathway has become a paradigm for both receptor kinase signaling in plants and steroid signaling by cell surface receptors in general.

show abstract

Section: Br Is Perceived By the Bri1 Receptor Kinase On Cell Surfacementioning

confidence: 99%

The brassinosteroid signal transduction pathway

Wang

Chong

et al. 2006

Cell Res

View full text Add to dashboard Cite

show abstract

“…Analysis of BS-deficient mutants in Arabidopsis reveals their essential role in plant development (Clouse, Langford & McMorris 1996;Kauschmann et al 1996). However, being produced by all tissues and modulating a very large range of processes, BSs differ from all other identified plant growth regulators (PGRs) (Clouse & Sasse 1998).…”

Section: Variability In the Response To Brassinosteroidsmentioning

confidence: 99%

“…For example, root elongation is inhibited by an exogenous supply of BS in Arabidopsis thaliana (Clouse et al 1996), mungbean (Guan & Roddick 1988a), tomato (Guan & Roddick 1988b), maize and wheat (Roddick & Ikekawa 1992). In cuttings of Phaseolus vulgaris, steroids also inhibit the emergence of adventitious roots (Hewitt & Hillman 1979).…”

Section: Variability In the Response To Brassinosteroidsmentioning

confidence: 99%

Data analysis in plant physiology: are we missing the reality?

Amzallag

2001

Plant Cell & Environment

View full text Add to dashboard Cite

In plant physiology, data analysis is based on the comparison of mean values. In this perspective, variability around the mean value has no significance per se , but only for estimating statistical significance of the difference between two mean values. Another approach to variability is proposed here, derived from the difference between redundant and deterministic patterns of regulation in their capacity to buffer noise. From this point of view, analysis of variability enables the investigation of the level of redundancy of a regulation pattern, and even allows us to study its modifications. As an example, this method is used to investigate the effect of brassinosteroids (BSs) during vegetative growth in Sorghum bicolor . It is shown that, at physiological concentrations, BSs modulate the network of regulation without affecting the mean value. Thus, it is concluded that the physiological effect of BSs cannot be revealed by comparison of mean values. This example illustrates how a part of the reality (in this case, the most relevant one) is hidden by the classical methods of comparison between mean values. The proposed tools of analysis open new perspectives in understanding plant development and the nonlinear processes involved in its regulation. They also ask for a redefinition of fundamental concepts in physiology, such as growth regulator, optimality, stress and adaptation.

show abstract

“…Defects in BR biosynthesis and signaling result in dwarfism, dark-green and rounded leaves, delayed development, reduced fertility and abnormal vascular pattern of plants [2][3][4][5][6]. Up to now, several key components of the BR signaling pathway have been identified through molecular and genetics studies.…”

Section: Introductionmentioning

confidence: 99%

Membrane steroid-binding protein 1 (MSBP1) negatively regulates brassinosteroid signaling by enhancing the endocytosis of BAK1

et al. 2009

View full text Add to dashboard Cite

Brassinosteroids (BRs) are perceived by transmembrane receptors and play vital roles in plant growth and development, as well as cell in responses to environmental stimuli. The transmembrane receptor BRI1 can directly bind to brassinolide (BL), and BAK1 interacts with BRI1 to enhance the BRI1-mediated BR signaling. Our previous studies indicated that a membrane steroid-binding protein 1 (MSBP1) could bind to BL in vitro and is negatively involved in BR signaling. To further elucidate the underlying mechanism, we here show that MSBP1 specifically interacts with the extracellular domain of BAK1 in vivo in a BL-independent manner. Suppressed cell expansion and BR responses by increased expression of MSBP1 can be recovered by overexpressing BAK1 or its intracellular kinase domain, suggesting that MSBP1 may suppress BR signaling through interacting with BAK1. Subcellular localization studies revealed that both MSBP1 and BAK1 are localized to plasma membrane and endocytic vesicles and MSBP1 accelerates BAK1 endocytosis, which results in suppressed BR signaling by shifting the equilibrium of BAK1 toward endosomes. Indeed, enhanced MSBP1 expression reduces the interaction between BRI1 and BAK1 in vivo, demonstrating that MSBP1 acts as a negative factor at an early step of the BR signaling pathway.

show abstract

A Brassinosteroid-Insensitive Mutant in Arabidopsis thaliana Exhibits Multiple Defects in Growth and Development

Cited by 748 publications

References 42 publications

The brassinosteroid signal transduction pathway

The brassinosteroid signal transduction pathway

Data analysis in plant physiology: are we missing the reality?

Membrane steroid-binding protein 1 (MSBP1) negatively regulates brassinosteroid signaling by enhancing the endocytosis of BAK1

Contact Info

Product

Resources

About