A brassinosteroid transcriptional network revealed by genome‐wide identification of BESI target genes in <i>Arabidopsis thaliana</i>

Yu, Xiaofei; Li, Lei; Żola, Jarosław; Aluru, Maneesha; Ye, Huaxun; Foudree, Andrew; Guo, Hongqing; Anderson, Sarah N.; Aluru, Srinivas; Liu, Peng; Rodermel, Steven R.; Yin, Yanhai

doi:10.1111/j.1365-313x.2010.04449.x

Cited by 601 publications

(678 citation statements)

References 84 publications

(172 reference statements)

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“…BES1 and BZR1 repress several target genes, including BR biosynthetic genes, light signalling components and chloroplast development-related genes 46,47 . However, it has been an open question as to how these two transcription factors repress their target genes.…”

Section: Discussionmentioning

confidence: 99%

Control of early seedling development by BES1/TPL/HDA19-mediated epigenetic regulation of ABI3

et al. 2014

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Seed germination and young seedling establishment should be tightly regulated to maximize plant survival and thereby enable successful propagation. Plants have evolved developmental signalling networks to integrate environmental cues for proper control of these critical processes, in which brassinosteroids are known to attenuate ABA-mediated arrest of early seedling development; however, the underlying regulatory mechanism remains elusive. Here we reveal that a BES1/TPL/HDA19 repressor complex mediates the inhibitory action of brassinosteroids on ABA responses during early seedling development. BR-activated BES1 forms a transcriptional repressor complex with TPL-HDA19, which directly facilitates the histone deacetylation of ABI3 chromatin. This event leads to the transcriptional repression of ABI3 and consequently ABI5, major ABA signalling regulators in early seedling development. Our data reveal that the BR-activated BES1-TPL-HDA19 repressor complex controls epigenetic silencing of ABI3 and thereby suppresses the ABA signalling output during early seedling development.

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Section: Discussionmentioning

confidence: 99%

Control of early seedling development by BES1/TPL/HDA19-mediated epigenetic regulation of ABI3

et al. 2014

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“…In Arabidopsis, BZR family proteins were thought to be the primary transcription factors regulating huge numbers of genes involved in BR signal output (Sun et al 2010;Yu et al 2011). Rice BZR family has been suggested to play a conserved role as in Arabidopsis (Tong and Chu 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Downstream, BZR1 (BRASSINA-ZOLE RESISTANT1) and BES1 (BRASSINOSTEROID INSENSITIVE 1-ETHYL METHANESULFONATE-SUPPRESSOR 1), two closely related TFs belonging to the BRASSINAZOLE-RESISTANT (BZR) TF family, are rapidly dephosphorylated by protein phosphatase 2A (PP2A) (Tang et al 2011). The dephosphorylated BZR1 and BES1 accumulate in the nucleus and directly bind to cis elements, known as E-box (CANNTG) and BRresponse element (CGTGT/CG) of their target, regulating plant growth and development (Yu et al 2011, Wu et al 2016. Although the interaction between stress and BRs has long been observed (Nawaz et al 2017), the underlying molecular mechanisms were far to be completely elucidated.…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of the BZR transcription factor family and its expression in response to abiotic stresses in Zea mays L.

et al. 2017

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Brassinosteroids (BRs) are plant specific steroidal hormones that play diverse roles in regulating a broad spectrum of plant growth and developmental processes, as well as, in responding to various biotic and abiotic stresses. Extensive research over the years has established stress-impact-mitigating role of BRs and associated compounds in different plants exposed to various abiotic and biotic stresses, suggesting the idea that they may act as immunomodulators, thus opening new approaches for plant resistance against hazardous environmental conditions. In this research the characterization of the transcriptional response of 11 transcription factors (TFs) belonging to BRASSINAZOLE-RESISTANT 1 (BZR1) TF family of Zea mays L. was analyzed in seedlings subjected to different stress conditions. Being important regulators of the BR synthesis, BZR TFs might have stress resistance related activities. However, no stress resistance related functional study of BZR TFs has been reported in maize so far. In silico analyses of the selected 11 TFs validated the features of their protein domains, where the highest degree of similarity observed with recognized BZR TFs of rice and Sorghum bicolor. Additionally, we investigated the organ-specific expression of 11 ZmBZR in maize seedlings. Five of them did not show any transcript accumulation, suggesting that ZmBZR expression might be regulated in a manner dependent on plant developmental stage. For the remaining six ZmBZR, their ubiquitous expression in the whole plant indicates they could function as growth regulators during maize development. More importantly, in response to various stress conditions, the spatial transcript accumulation of all ZmBZR varies along the plant. All six ZmBZR showed up-regulation against N starvation, hypoxia and salt stress. On the contrary, heat stress clearly down-regulated gene expression of all ZmBZR analysed. Consistently with the expression results, the distribution of stress-related cis-acting elements in the promoter of these genes inferred that the maize BZR TFs might play some roles in regulating the expression of the corresponding genes in response to multifarious stresses. In conclusion, these data reveal that BZR TFs have stress signaling activity in maize, in addition to their confirmed role in regulating plant physiology and morphology.

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“…Via sequential transphosphorylation events within the BRI1-BAK1 (SERK3) heterooligomers, BRI1 eventually gains full kinase activity and downstream BR signaling is initiated Kim and Wang, 2010;Clouse, 2011;Jaillais et al, 2011a). A phosphorylation and dephosphorylation cascade relays the signal of BR binding through BR signaling kinases (Tang et al, 2008b;Kim et al, 2009) or CONSTITUTIVE DIFFERENTIAL GROWTH1 (CDG1) and CDG1-LIKE1 to downstream targets, culminating in the transcriptional regulation of BR-responsive genes (Kim and Wang, 2010;Sun et al, 2010;Yu et al, 2011) mediated by the BRASSINAZOLE-RESISTENT1 (BZR1) and bri1-EMS SUPPRESSOR1 (BES1; also known as BZR2) transcription factors Wang et al, 2002;Yin et al, 2002Yin et al, , 2005He et al, 2005).…”

mentioning

confidence: 99%

Visualization of BRI1 and BAK1(SERK3) Membrane Receptor Heterooligomers during Brassinosteroid Signaling

Bücherl¹,

Esse²,

Kruis³

et al. 2013

Plant Physiology

110

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The leucine-rich repeat receptor-like kinase BRASSINOSTEROID-INSENSITIVE1 (BRI1) is the main ligand-perceiving receptor for brassinosteroids (BRs) in Arabidopsis (Arabidopsis thaliana). Binding of BRs to the ectodomain of plasma membrane (PM)-located BRI1 receptors initiates an intracellular signal transduction cascade that influences various aspects of plant growth and development. Even though the major components of BR signaling have been revealed and the PM was identified as the main site of BRI1 signaling activity, the very first steps of signal transmission are still elusive. Recently, it was shown that the initiation of BR signal transduction requires the interaction of BRI1 with its SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) coreceptors. In addition, the resolved structure of the BRI1 ectodomain suggested that BRI1-ASSOCIATED KINASE1 [BAK1](SERK3) may constitute a component of the ligand-perceiving receptor complex. Therefore, we investigated the spatial correlation between BRI1 and BAK1(SERK3) in the natural habitat of both leucine-rich repeat receptor-like kinases using comparative colocalization analysis and fluorescence lifetime imaging microscopy. We show that activation of BR signaling by exogenous ligand application resulted in both elevated colocalization between BRI1 and BAK1(SERK3) and an about 50% increase of receptor heterooligomerization in the PM of live Arabidopsis root epidermal cells. However, large populations of BRI1 and BAK1(SERK3) colocalized independently of BRs. Moreover, we could visualize that approximately 7% of the BRI1 PM pool constitutively heterooligomerizes with BAK1(SERK3) in live root cells. We propose that only small populations of PMlocated BRI1 and BAK1(SERK3) receptors participate in active BR signaling and that the initiation of downstream signal transduction involves preassembled BRI1-BAK1(SERK3) heterooligomers.

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A brassinosteroid transcriptional network revealed by genome‐wide identification of BESI target genes in Arabidopsis thaliana

Cited by 601 publications

References 84 publications

Control of early seedling development by BES1/TPL/HDA19-mediated epigenetic regulation of ABI3

Control of early seedling development by BES1/TPL/HDA19-mediated epigenetic regulation of ABI3

Identification and characterization of the BZR transcription factor family and its expression in response to abiotic stresses in Zea mays L.

Visualization of BRI1 and BAK1(SERK3) Membrane Receptor Heterooligomers during Brassinosteroid Signaling

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