A brief review of CD163 and its role in PRRSV infection

Welch, Siao-Kun W.; Calvert, Jay G.

doi:10.1016/j.virusres.2010.07.018

Cited by 96 publications

(84 citation statements)

References 38 publications

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“…3A). In addition, we also monitored the expression of CD163, a scavenger receptor critical to PRRSV cell entry, and an M2-IL-10 cell marker (2,26). Previous studies reported 60% to 95% CD163-positive cells in porcine alveolar Ms using indirect immunodetection of CD163 (26,42).…”

Section: Resultsmentioning

confidence: 99%

“…In addition, we also monitored the expression of CD163, a scavenger receptor critical to PRRSV cell entry, and an M2-IL-10 cell marker (2,26). Previous studies reported 60% to 95% CD163-positive cells in porcine alveolar Ms using indirect immunodetection of CD163 (26,42). To study CD163 expression dynamics and correlate CD163 expression data detected using either direct or indirect immunodetection, we applied both detection procedures in alveolar Ms in different culture and activation states.…”

Section: Resultsmentioning

confidence: 99%

“…PRRSV is an enveloped positive-strand RNA virus that directly infects subsets of Ms and DCs and subverts immune responses in monocytic cells, making it ideal for deciphering how monocytic cell activation status interacts with antiviral immunity (21,(24)(25)(26)(27)(28). Monocytic cells are critical for providing early immune surveillance and bridging adaptive antiviral immunity (1-5, 7, 11).…”

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confidence: 99%

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Antiviral Regulation in Porcine Monocytic Cells at Different Activation States

Sang

Rowland

Blecha

2014

J Virol

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IMPORTANCE Activation statuses of monocytic cells, including monocytes, macrophages (Ms), and dendritic cells (DCs), are critically important for antiviral immunity. Unfortunately, the activation status of porcine monocytic cells or how cell activation status functionally interacts with antiviral immunity remains largely unknown. This is a significant omission because many economically important porcine viruses are monocytotropic, including our focus, PRRSV, which alone causes nearly $800 million economic loss annually in the U.S. swine industries. PRRSV is ideal for deciphering how monocytic cell activation statuses interact with antiviral immunity, because it directly infects subsets of monocytic cells and subverts overall immune responses. In this study, we systematically investigate the activation status of porcine monocytic cells to determine the intricate interaction of viral infection with activation statuses and functionally regulate antiviral immunity within the framework of the activation paradigm. Our findings may provide a means of potentiating antiviral immunity and leading to novel vaccines for PRRS prevention.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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Antiviral Regulation in Porcine Monocytic Cells at Different Activation States

Sang

Rowland

Blecha

2014

J Virol

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“…The ASFV uses CD163 as a binding and internalization receptor, while for PPRSV, CD163 also plays a role in the uncoating process [57,58]. The susceptibility to infection for both viruses is proportional to the expression of CD163 on the cell surface [57,58], and the viruses have a very restricted cell tropism for the monocyte/macrophage cells [57,58,61]. In a study of swine bone marrow monocyte precursors, peripheral blood monocytes and alveolar macrophages, the susceptibility of infection by ASFV was dependent on the degree of maturation of mononuclear phagocytes, being the lowest in bone marrow cells and the greatest in alveolar macrophages [57].…”

Section: F F F F Function In Inflammation Unction In Inflammation Uncmentioning

confidence: 99%

“…Moreover, in vitro maturation of peripheral blood monocytes, which is associated with up-regulation of CD163, led to increased susceptibility of those in vitro matured cells to ASFV infection [57]. Although the natural host for the PPRSV is the pig, expression of dog, mouse, monkey or human CD163 can render cells at least partially permissive for PPRSV infection and replication [58,61]. A series of mutation and genetic recombination studies have demonstrated that the fifth, but not the first, second or ninth, SRCR domain of CD163 is crucial for binding and internalization of PPRSV [62].…”

Section: F F F F Function In Inflammation Unction In Inflammation Uncmentioning

confidence: 99%

CD163 and its role in inflammation

Kowal¹,

Silver²,

Sławińska³

et al. 2011

Folia Histochem Cytobiol.

124

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Abstract:Abstract: Abstract: Abstract: Abstract: Mononuclear phagocytes represent a heterogeneous population of cells with individual subpopulations exerting different pro-or anti-inflammatory functions. CD163 is a monocyte/macrophage specific marker expressed predominantly on cells which possess strong anti-inflammatory potential. The expression of CD163 is strongly induced by anti-inflammatory mediators such as glucocorticoids and interleukin-10, while being inhibited by pro-inflammatory mediators such as interferon-gamma. CD163-expressing mononuclear phagocytes, as well as soluble CD163, may both take part in downregulating an inflammatory response. It seems, therefore, that CD163 may be an interesting target for therapeutic modulation of the inflammatory response. (Folia Histochemica et Cytobiologica 2011, Vol. 49, No. 3, 365-374)

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Gene editing as applied to prevention of reproductive porcine reproductive and respiratory syndrome

Whitworth

Prather

2017

Molecular Reproduction Devel

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Funding informationGenus plc, DeForest, WI Porcine Reproductive and Respiratory Syndrome (PRRS) causes severe reproductive failure in sows as well as transplacental transfer of PRRS virus (PRRSV) to late-gestation fetuses, resulting in abortions, early farrowing, increased number of stillborn piglets, and weak neonatal piglets.PRRSV-infected boars present with anorexia and lethargy, and have decreased sperm quality.The gene for the cellular receptor that the PRRSV uses, Cluster of differentiation 163 (CD163), was edited using Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 geneediting technology to create biallelic DNA edits to the CD163 gene in 100% of the offspring.CD163-null pigs challenged with virus were completely resistant to both Type 1 and Type 2 PRRSV isolates, as measured by clinical signs, viremia, antibody response, and lung histopathology. In vitro studies showed that CD163-null alveolar macrophages were also not permissive to infection by a panel of six Type 1 and nine Type 2 viral isolates. Thus, DNA editing of the CD163 gene prevented PRRSV infection and reproductive losses associated with infection. K E Y W O R D SCD163, CRISPR/Cas9, genetic engineering, zygote injection[S]ite-directed nucleases opened the door to efficiently produce pigs with specific DNA edits that can now be used as models to improve many aspects of animal agriculture.

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A brief review of CD163 and its role in PRRSV infection

Cited by 96 publications

References 38 publications

Antiviral Regulation in Porcine Monocytic Cells at Different Activation States

Antiviral Regulation in Porcine Monocytic Cells at Different Activation States

CD163 and its role in inflammation

Gene editing as applied to prevention of reproductive porcine reproductive and respiratory syndrome

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