A Brief Review of circRNA Biogenesis, Detection, and Function

Liang, Ying; Liu, Niannian; Yang, Le; Tang, Jianjun; Wang, Yinglong; Meng, Ming

doi:10.2174/1389202922666210331130722

Cited by 29 publications

(22 citation statements)

References 77 publications

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“…The majority of circRNAs are non‐coding, highly conserved, and predominantly located in the cytoplasm, with only a limited number found in the nucleus 31 . While a minority of circRNAs participate in transcription, the majority are involved in the regulation of transcription and post‐transcriptional processes 32 …”

Section: Characteristicsmentioning

confidence: 99%

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CircRNA‐associated ceRNA regulatory networks as emerging mechanisms governing the development and biophysiopathology of epilepsy

Kohansal,

Alghanimi,

Banoon

et al. 2024

CNS Neurosci Ther

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The etiology of epilepsy is ascribed to the synchronized aberrant neuronal activity within the brain. Circular RNAs (circRNAs), a class of non‐coding RNAs characterized by their circular structures and covalent linkage, exert a substantial influence on this phenomenon. CircRNAs possess stereotyped replication, transience, repetitiveness, and paroxysm. Additionally, MicroRNA (miRNA) plays a crucial role in the regulation of diverse pathological processes, including epilepsy. CircRNA is of particular significance due to its ability to function as a competing endogenous RNA, thereby sequestering or inhibiting miRNA activity through binding to target mRNA. Our review primarily concentrates on elucidating the pathological and functional roles, as well as the underlying mechanisms, of circRNA–miRNA–mRNA networks in epilepsy. Additionally, it explores the potential utility of these networks for early detection and therapeutic intervention.

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Section: Characteristicsmentioning

confidence: 99%

“…31 While a minority of circRNAs participate in transcription, the majority are involved in the regulation of transcription and posttranscriptional processes. 32…”

Section: Char Ac Teris Tic Smentioning

confidence: 99%

CircRNA‐associated ceRNA regulatory networks as emerging mechanisms governing the development and biophysiopathology of epilepsy

Kohansal,

Alghanimi,

Banoon

et al. 2024

CNS Neurosci Ther

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“…Recent studies have shown that circRNAs play an important role in their occurrence and have become a research hotspot. [3][4][5] circRNA plasmacytoma variant translocation 1 (circPVT1), transcribed from the long noncoding RNA region with PVT1 locus on chromosome 8q24, is upregulated to drive the development of various cancers. 6,7 PVT1 from online data analysis was abnormally expressed in BLCA.…”

Section: Introductionmentioning

confidence: 99%

circPVT1 Inhibits the Proliferation and Aids in Prediction of the Prognosis of Bladder Cancer

Zhou,

Cui,

Zhu

et al. 2024

PGPM

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Background: Circular RNA PVT1 (circPVT1) is aberrantly expressed in several cancers, but its functional role and clinical relevance in bladder urothelial carcinoma (BLCA) remain unknown. This study aimed to identify the expression level of circPVT1 in BLCA and investigated its functional relevance with BLCA progression both in vitro and in vivo. Methods: GEPIA, UALCAN, and OncoLnc were referred to presented data. Quantitative real-time PCR (qPCR) was used for the measurement of transnational expression of genes in BLCA specimens and cell lines. Immunohistochemistry (IHC) and fluorescence in situ hybridization analysis (FISH) assays were performed to detect HER2 amplification, Pearson's correlation analysis to analyze the correlation between circPVT1 expression and clinical characteristics, Cox regression and K-M survival analyses to analyze prognostic factors. A nomogram was constructed for predicting prognosis. The proliferation of cells was measured by CCK-8 and colony formation assay, and the proliferation in vivo was evaluated using nude mouse models. qPCR was used to detect the expression of proliferation-related genes. Results: circPVT1 was but mRNA PVT1 was not significantly overexpressed in BLCA. A high circPVT1 expression was associated with a better survival and negative HER2, but not with age, gender, and T stage. circPVT1 was an independent prognostic factor for the overall survival of BLCA patients. Knocking down circPVT1 promoted BLCA proliferation in vitro and in vivo. Knocking down circPVT1 upregulated ERBB2, MKI67, and PCNA expression and downregulated TP53 expression, but exerted no influence on CCND1 and CCNB1 expression. Conclusion: circPVT1 is a tumor suppressor and novel prognostic biomarker for BLCA.

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“…Circular RNAs (circRNAs) are a special class of non-coding RNA (ncRNA) molecules, which have a closed ring structure without 3'- and 5'-ends compared with linear RNA ( 7 ). Studies have shown that circRNAs can act as competing endogenous RNAs (ceRNAs) to decrease the inhibition of miRNAs on their target genes and promote target gene expression in cells ( 8 , 9 ).…”

Section: Introductionmentioning

confidence: 99%

CircRNA_0050486 promotes cell apoptosis and inflammation by targeting miR-1270 in atherosclerosis

Wang¹,

Bai²,

Mei³

et al. 2022

Ann Transl Med

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Background Atherosclerosis (AS) is a chronic inflammatory disease that plays a major role in cardiovascular disease. Circular RNAs (circRNAs) are related to the pathogenesis of AS, including the inflammatory response. This study aimed to explore the underlying mechanisms of circRNAs and how they regulate the inflammatory response in AS. Methods Analyzed the expression profile of circRNAs in three oxidized low-density lipoprotein (oxLDL) treated macrophage samples and three macrophage control samples using bioinformatics methods. Expression and biological function of circRNA were verified in oxLDL-induced THP-1 macrophages. MiRNAs and target genes of circRNA were predicted by functional enrichment analysis. Expression and function of circRNA target miRNAs were explored in oxLDL-induced THP-1 macrophages. Finally, we predicted and analyzed the function of circRNAs-miRNAs target genes in AS. Results We identified nine upregulated circRNAs and found that circ_0050486 was significantly upregulated in a THP-1 + PMA + oxLDL group compared with a THP-1 + PMA group. Additionally, circ_0050486 knockdown markedly inhibited IL-6 and TNF-α concentrations and the cell death rates in oxLDL-induced THP-1 macrophages. Furthermore, circ_0050486 targeted and inhibited miR-145 and miR-1270 . Upregulated miR-1270 markedly inhibited IL-6 and TNF-α levels and the cell death rates in oxLDL-induced THP-1 macrophages. Finally, the target genes of miR-1270 and miR-145 were predicted by the miRDB, miRWalk, and Targetscan databases, and a functional analysis network of the target genes was constructed by Cytoscape GlueGO, including the regulation of the immune response and monocyte chemotaxis. The common target genes of miR-145 and miR-1270 were established by Cytoscape and included NF1A, among others. Conclusions Our study suggested that circ_0050486 knockdown inhibited inflammation and apoptosis by targeting miR-1270 in oxLDL-induced THP-1 macrophages. This finding may provide a potential therapeutic target for atherosclerosis.

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A Brief Review of circRNA Biogenesis, Detection, and Function

Cited by 29 publications

References 77 publications

CircRNA‐associated ceRNA regulatory networks as emerging mechanisms governing the development and biophysiopathology of epilepsy

CircRNA‐associated ceRNA regulatory networks as emerging mechanisms governing the development and biophysiopathology of epilepsy

circPVT1 Inhibits the Proliferation and Aids in Prediction of the Prognosis of Bladder Cancer

CircRNA_0050486 promotes cell apoptosis and inflammation by targeting miR-1270 in atherosclerosis

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