A brief Review on Q-absorption Ratio Method in UV-Spectrophotometry

Vyas, Amitkumar J.; Patel, Brijesh H.; Patel, Ashok B.; Patel, Nilesh K.

doi:10.52711/2231-5675.2022.00046

AJPA

2022

DOI: 10.52711/2231-5675.2022.00046

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A brief Review on Q-absorption Ratio Method in UV-Spectrophotometry

Amitkumar J. Vyas

Brijesh H. Patel

Ashok B. Patel

et al.

Abstract: This present work carries a brief review on Q-Absorption ratio method in UV-spectrophotometry. Absorption ratio method is used for the ratio of the absorption at two selected wavelengths one of which is iso-absorptive point and other being the λmax of one of the two components. The aim of this article is to give brief idea about various method developed using Q-Absorption ratio methodon combined dosage forms. It shows selection of wavelength, linearity study and choosing solvent for give combine dosage form. T… Show more

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Cited by 6 publications

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Separation & Purification Reviews

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Pharmaceutical Analysis of Berberine: An Update

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Separation & Purification Reviews

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UV-Visible Spectroscopy: A Review on its Pharmaceutical and Bio-allied Sciences Applications

Singhal,

Saini,

Chopra

et al. 2024

CPA

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Ultraviolet-Visible (UV-Vis) spectroscopy has emerged as a powerful analytical tool with diverse applications in pharmaceutical and bio-allied sciences. This article provides a comprehensive overview of the extensive utility of UV-Vis spectroscopy, emphasizing its pivotal role in characterizing and analyzing various compounds critical for drug development and bio-allied research. In the pharmaceutical sector, UV-Vis spectroscopy is a fundamental technique for quantifying the concentrations of active pharmaceutical ingredients (APIs) in formulations. Its non-destructive nature and high sensitivity make it an indispensable tool for quality control, ensuring the consistency and potency of pharmaceutical products. Furthermore, this technique has been employed in the study of drug-receptor interactions to elucidate the molecular mechanisms underlying therapeutic effects. In bio-allied applications, UV-Vis spectroscopy is used to analyze biomolecules like proteins, nucleic acids, and enzymes. This technique allows for the study of protein conformational changes, DNA structure, and enzymatic activity, offering crucial insights into fundamental biological processes. Additionally, UV-Vis spectroscopy aids in determining biomarker concentrations, assisting in the early diagnosis and monitoring of various diseases. This article also explores recent advancements in UV-Vis spectroscopy, including the integration of nanomaterials and computational approaches to enhance sensitivity and selectivity. Moreover, it discusses the potential of UV-Vis spectroscopy in emerging areas such as personalized medicine and point-of-care diagnostics. As technology continues to evolve, UV-Visible spectroscopy is poised to significantly contribute to the ever-expanding landscape of pharmaceutical and bio-related research.

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Simultaneous Estimation of Naltrexone Hydrochloride and Zonisamide in Synthetic Mixture by RP-HPLC Method

Dhaduk,

K. Patel,

B. Patel

et al. 2024

RJPT

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A simple, rapid, precise, and reproducible RP-HPLC method development and validation done for simultaneous estimation of Naltrexone Hydrochloride and Zonisamide in synthetic mixture. The isocratic method was developed usinɡ a Water Symmetry C18 (250 mm X 4.6 mm, 5 µm) column. Mobile phase composition was Phosphate Buffer: Methanol (75:25 %V/V) (Buffer-1.36 ɡ of KH2PO4 in 1000 ml adjust pH 3.0 ± 0.05 with OPA.), flow rate 1 ml/min and detection carried out at 230 nm at column temperature 30ºC. The injection volume was 10µl with run time of 10 minutes. Parameters such as linearity, precision, accuracy, recovery, specificity and robustness are performed as per ICH ɡuidelines Q2(R1). Retention time of Naltrexone Hydrochloride and Zonisamide were found to be 3.92 min and 8.15 min respectively. The developed method found linear in the ranɡe of 1-10 µɡ/ml for Naltrexone Hydrochloride and 2.84-142 µɡ/ml for Zonisamide (R2 = 0.9999 for both druɡs). The % Recovery were found to be 99.39 %-100.70 % and 98.91 %-100.59 % for Naltrexone Hydrochloride and Zonisamide respectively. The RSD for both druɡ were less than 2 for precision, specificity and robustness.

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A brief Review on Q-absorption Ratio Method in UV-Spectrophotometry

Cited by 6 publications

References 25 publications

Pharmaceutical Analysis of Berberine: An Update

Pharmaceutical Analysis of Berberine: An Update

UV-Visible Spectroscopy: A Review on its Pharmaceutical and Bio-allied Sciences Applications

Simultaneous Estimation of Naltrexone Hydrochloride and Zonisamide in Synthetic Mixture by RP-HPLC Method

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