A broad-spectrum antimicrobial activity of thermophilic Nocardiopsis sp. producing multiple extracellular enzymes of industrial and therapeutic use

Begani, Jyotsna; Lakhani, Jyoti; Harwani, Dharmesh

doi:10.31024/ajpp.2019.5.3.14

Cited by 1 publication

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was kept for shaking on an orbital shaker for 30 min at 37°C and serial dilutions were made. From each dilution 0.1 mL of sample was spread on different selective isolation media namely ISP2 (International Streptomycete Project), [30] Benedict's modification of the Lindenbein [31] , Czapek's [32] , Gauze's [33] , humic-acid vitamin [34][35] potassium-tellurite [36] , raffinose-histidine [37] , R2YE [38] , starch-casein [39] and MAS [18] agar media. The antibiotics kanamycin, nalidixic acid and cyclohexamide (25µg/ml) were added in the media to inhibit bacterial and fungal contaminations, respectively.…”

Section: Materials and Methods Soil Sampling And Isolation Of Actinobacteriamentioning

confidence: 99%

See 1 more Smart Citation

Sand Dune Streptomyces JB66 Native to the Great Indian Thar Desert Inhibits Multidrug-Resistant Pathogens

Begani

Lakhani

Harwani

2019

Int. J. Pharm. Sci. Drug Res.

Self Cite

View full text Add to dashboard Cite

The efficacy of an actinomycete strain JB66 recovered from a sand dune soil from the Bikaner district of the Thar desert in inhibiting the growth of various bacterial pathogens was studied. The type strains Staphylococcus aureus, Shigella flexneri, Klebsiella pneumoniae and the clinical isolates Escherichia coli, multidrug-resistant S. aureus and P. vulgaris were included in the antimicrobial assays. Polyphasic characterization of JB66 isolate revealed its identity as Streptomyces (MH762010). It showed 88.99-89.24% sequence similarity with the other members of this genus and share the maximum (88.89%) similarity with Streptomyces sp. ATSC13. The strain JB66 was found to produce a high amount of extracellular L-asparaginase, catalase, gelatinase, protease, tyrosinase and urease enzymes. The partial chemical categorization of the methanolic crude extract of the JB66 strain led to the preliminary identification of various metabolic compounds. The thin-layer chromatography fractionation revealed the presence of prodigiosin pigment or chandramycin, cephalosporin or zeatin, daidzein, demethoxy rapamycin, 4,6-dihydroxy-7-methoxyisoflavone, munumbicins and amiclenomycin like compounds. Bio-autography revealed that the metabolites localized at the Rf values of 0.40, 0.46 and 0.53 in TLC profile had the actual bioactive fractions. UV-VIS spectrum absorbance maxima at 288 nm revealed the presence of an aromatic nucleus.

show abstract

Section: Materials and Methods Soil Sampling And Isolation Of Actinobacteriamentioning

confidence: 99%

“…. [5,[17][18] Due to the emergence of multidrug-resistant pathogens against existing antibiotics, it is highly important to find the novel source of antibiotics and alternate methods on an urgent basis. By exploring the unexplored or less explored environment, the possibility of finding new biologically active metabolites can be increased.…”

Section: Introductionmentioning

confidence: 99%