A bZIP transcription factor is involved in regulating lipid and pigment metabolisms in the green alga Chlamydomonas reinhardtii

A conserved MYB transcription factor is involved in regulating lipid metabolic pathways for oil biosynthesis in green algae

“…It has been proposed that transcription factors (TFs) contribute to the regulation of algal lipid metabolic pathways for TAG homeostasis (Hu et al ., 2014; Gargouri et al ., 2015; Liu et al ., 2019). There are only a few reports about the characterization of algal TFs involved in lipid metabolism, which are mainly from C. reinhardtii ; for example, the squamosa promoter binding protein TF Nitrogen Response Regulator1 (Boyle et al ., 2012), the cysteine‐rich polycomb‐like protein TF Compromised Hydrolysis of Triacylglycerols 7 (Tsai et al ., 2014), the G2‐like TF Phosphorus Starvation Response1 (Ngan et al ., 2015; Bajhaiya et al ., 2016), the myeloblastosis (MYB) or MYB‐like TFs, including ROC40 (Goncalves et al ., 2016), Lipid Remodeling Regulator1 (LRL1) (Hidayati et al ., 2019), and Cell Division Cycle 5 (CDC5) (Torres‐Romero et al ., 2020), and the basic leucine zipper (bZIP) TFs CrbZIP1 (Yamaoka et al ., 2019) and CrbZIP2 (Bai et al ., 2021). Nevertheless, the target genes and binding sites recognized by these TFs and underlying regulatory mechanisms remain less understood.…”

Non-Tandem CCCH-Type Zinc-Finger Protein CpZF_CCCH1 Improves Fatty Acid Desaturation and Stress Tolerance in Chlamydomonas reinhardtii

“…However, the MGDG and MGMG species, along with the SQDG and sulfoquinovosylmonoacylglycerol (SQMG) species, continuously declined in both ZF-1 and EV under nitrogen deprivation (Figure D,F), suggesting their primary contribution in supplying the DAG or glycerol skeleton. In contrast, the DGDG and 16:0- digalactosylmonoacylglycerol (DGMG) levels were increased slightly in both ZF-1 and EV under nitrogen deprivation (Figure E), suggesting the presence of lipases responsible for the degradation of DGDG . Concerning phospholipids, DGTS, and corresponding lysolipids, all abundances declined continuously but were still higher in ZF-1 than in EV (Figure G–M).…”

Involvement of Transcription Factors and Regulatory Proteins in the Regulation of Carotenoid Accumulation in Plants and Algae

“…In C. reinhardtii, CrbZIP2 disruption could impair triacylglycerol accumulation but promote polar lipids and more carotenoids and chlorophylls, suggesting its involvement in regulating lipid and pigment metabolisms . However, the exact mechanism of its regulation in carotenoid metabolism is unclear.…”

“…63 In C. reinhardtii, CrbZIP2 disruption could impair triacylglycerol accumulation but promote polar lipids and more carotenoids and chlorophylls, suggesting its involvement in regulating lipid and pigment metabolisms. 64 However, the exact mechanism of its regulation in carotenoid metabolism is unclear. Another Chlamydomonas bZIP transcription factor BLZ8 could regulate the expression of some genes involved in the carbon-concentrating mechanism (CCM), including HIGH-LIGHT ACTIVATED 3 (HLA3, bicarbonate transporter), CARBONIC ANHYDRASE 7 (CAH7), and CAH8, indicating that Chlamydomonas BLZ8 can help increase the CO 2 supply for photosynthesis by inducing CCM, thereby suppressing reactive oxygen species (ROS) production.…”