A calcium binding protein, Calbindin-D9k, is mainly regulated by estrogen in the pituitary gland of rats during estrous cycle

Nguyen, Thi Hoa; Lee, Geun‐Shik; Ji, Youn-Kyu; Choi, Kyung‐Chul; Lee, Chang Kyu; Jeung, Eui‐Bae

doi:10.1016/j.molbrainres.2005.09.008

Cited by 33 publications

(31 citation statements)

References 40 publications

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“…This study also established that the effects of single or combined treatment with OP and IBP in the GH3 rat pituitary cancer cell line were mediated by ER signaling. Our previous study demonstrated that the CaBP-9k gene is a useful biomarker for the estrogenicity of EDs in GH3 cells (13,14,(35)(36)(37)40). In general, the activity of alkylphenolic compounds (APs) is highly dependent on the alkyl substitutions present in those compounds.…”

Section: Discussionmentioning

confidence: 99%

“…Pituitary CaBP-9k expression is regulated during the estrous cycle. A significant increase in CaBP-9k expression is induced by E2 treatment in rats (36). In GH3 cells, it is well documented that estrogen activates CaBP-9k expression through an estrogen-responsive element (ERE) located in the 5'-upstream regulatory region of the gene (50).…”

Section: Discussionmentioning

confidence: 99%

“…E2 was shown to increase CaBP-9k gene expression in the rat pituitary gland. The rat GH3 cell line is a widely used pituitary somatolactotrophic cell line that is stimulated by estrogen and secretes prolactin (10,35,36). Therefore, the GH3 cell line is a good model for investigating the estrogenicity of EDs in vitro.…”

Section: Introductionmentioning

confidence: 99%

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Synergistic effects of octylphenol and isobutyl paraben on the expression of calbindin-D9k in GH3 rat pituitary cells

Kim

Jung²,

Choi³

et al. 2011

Int J Mol Med

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Abstract. Endocrine disruptors (EDs) have estrogenic activity and can cause physiological estrogenic responses. Octylphenol (OP) is one of the alkylphenolic compounds known as environmental xenoestrogens because they strongly compete with endogenous estrogens to bind to estrogen receptors (ERs). Isobutyl paraben (IBP), a widely used preservative, also exhibits estrogenic activity. Calbindin-D 9k (CaBP-9k) is a novel biomarker for the detection of EDs used in our previous studies. In this study, the CaBP-9k gene was utilized as a marker for the estrogenic activity of combined OP and IBP to investigate possible additive, synergistic or antagonistic effects of these compounds in GH3 rat pituitary cells. GH3 cells were treated with different individual or combined doses of OP and IBP. In addition, the antiestrogen ICI 182,780 was used to examine the potential involvement of ERs in the induction of CaBP-9k expression by EDs. It was found that CaBP-9k expression was significantly increased at a high-dose of OP (1 µM) combined with each dose of IBP (0.1, 1 or 10 µM) compared to all single doses of IBP and OP. A synergistic increase in luciferase activity and CaBP-9k expression was observed following combination treatment with OP and IBP. Expression of the progesterone receptor (PR) gene was similarly induced by combined treatment with OP and IBP. In addition, pre-treatment with ICI 182,780, an estrogen antagonist, significantly blunted ED-induced CaBP-9k and PR expression. In summary, the expression of CaBP-9k and PR was induced more potently by combined OP and IBP than by treatment with either ED alone. ICI 182,780 treatment reversed ED-induced CaBP-9k and PR expression in these cells. Taken together, these results indicate that combined exposure to OP and IBP has a synergistic effect on the induction of CaBP-9k and PR gene expression via an ER-dependent pathway in GH3 cells.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Synergistic effects of octylphenol and isobutyl paraben on the expression of calbindin-D9k in GH3 rat pituitary cells

Kim

Jung²,

Choi³

et al. 2011

Int J Mol Med

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“…The rats were euthanized 48 h after the last injection. In both experiments, the rat pituitary glands were isolated following euthanization for further RNA and protein extractions as previously described [28]. …”

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confidence: 99%

“…Interactions between these ERs and estrogen responsive elements (ERE) in the promoters of many genes then leads to the regulation of gene expression [43]. Functionally, ERs mediate pituitary cell responsiveness to estrogens [28] and thus, estrogen may influence GH3 cell growth and increase PRL release [9]. In addition, co-treatment with ICI 182780 antagonizes E2-induced PRL secretion [44], suggesting that ERs play a physiological role in mediating PRL release.…”

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confidence: 99%

Estrogen Receptors are Involved in Xenoestrogen Induction of Growth Hormone in the Rat Pituitary Gland

Dang

Choi

Jeung

2009

Journal of Reproduction and Development

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Abstract. Growth hormone (GH) plays a pivotal role in the regulation of growth, development and body composition. In order to provide new insights into estrogenic endocrine disruptor (ED) activities in the pituitary gland and the potential role played by estrogen receptors (ERs) in mediating their effects in vivo, we examined GH expression in the pituitary gland of an immature rat model. At postnatal day 14, immature rats were treated with various doses of 4-tertoctylphenol (OP), p-nonylphenol (NP) and bisphenol A (BPA), and the GH mRNA and protein expression levels were analyzed by real-time quantitative PCR and western blot/immunohistochemistry (IHC), respectively. An anti-estrogen (ICI 182780) was used to examine the potential involvement of ERs in ED-induced GH expression during critical windows of development. GH mRNA expression increased significantly 48 h after treatment with a high dose (600 mg/ kg body weight [BW]) of OP or NP. However, this induction was abolished completely by co-treatment with ICI 182780. No significant difference in GH mRNA expression was observed following treatment with BPA or co-treatment of BPA with the anti-estrogen. Exposure to high doses (600 mg/kg BW) of these EDs significantly enhanced GH protein expression in the rat pituitary gland, whereas pretreatment with ICI 182780 markedly reduced this expression. Taken together, we have demonstrated for the first time that in vivo exposure to EDs can induce GH mRNA and protein expression in the rat pituitary gland and that their activities may involve an ER-mediated signaling pathway. These results may provide critical evidence for ED-induced dysregulation of pituitary GH expression and thus may be important for elucidating the potential impacts of EDs in altered body growth and development and for predicting the health risks of ED exposure in humans and wildlife. Key words: Endocrine disruptor, Estrogenicity, Growth hormone, Pituitary gland, Rat (J. Reprod. Dev. 55: [206][207][208][209][210][211][212][213] 2009) rowth hormone (GH) plays a pivotal role in growth and development during puberty and is a member of a family of evolutionarily-related hormones that include prolactin (PRL) and placental lactogen [1]. In addition, GH performs important functions in metabolism and body composition [2]. GH activity can be regulated directly via sex steroids, which control GH release, or indirectly by modulation of insulin-like growth factor (IGF)-I [3]. Although most of its growth-promoting activity is mediated by IGF-I activation [1], studies have indicated that changes in circulating levels of sex steroids may lead to modulation of GH synthesis and release of GH from the anterior lobe of the pituitary [4]. In humans, GH regulates growth in childhood [5], and most endocrine disorders that influence body growth and development affect GH levels during this critical window. Recent reports have indicated a positive correlation between GH and estrogen levels in prepubertal girls and boys [6,7]. The effects of estrogen stimulation on growth dep...

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The astroglial and stem cell functions of adult rat folliculostellate cells

Fletcher

Smiljanić

Prévide

et al. 2022

Glia

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The mammalian pituitary gland is a complex organ consisting of hormone-producing cells (HPC), nonhormonal folliculostellate cells (FSC) and pituicytes, vascular pericytes and endothelial cells, and putative Sox2-expressing stem cells. Here, we used scRNAseq analysis of adult female rat pituitary cells to study the heterogeneity of pituitary cells with a focus on evaluating the transcriptomic profile of the Sox2expressing population. Samples containing whole pituitary and separated anterior and posterior lobe cells allowed the identification of all expected pituitary resident cell types and lobe-specific subpopulations of vascular cells. Sox2 was expressed uniformly in all FSC, pituicytes, and a fraction of HPC. FSC comprised two subclusters; FSC1 contained more cells but expressed less genetic diversity compared to FSC2. The latter contained proliferative cells, expressed genes consistent with stem cell niche formation, including tight junctions, and shared genes with HPC. The FSC2 transcriptome profile was also consistent with the activity of pathways regulating cell proliferation and stem cell pluripotency, including the Hippo and Wnt pathways. The expression of other stem cell marker genes was common for FSC and pituicytes (Sox9,

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A calcium binding protein, Calbindin-D9k, is mainly regulated by estrogen in the pituitary gland of rats during estrous cycle

Cited by 33 publications

References 40 publications

Synergistic effects of octylphenol and isobutyl paraben on the expression of calbindin-D9k in GH3 rat pituitary cells

Synergistic effects of octylphenol and isobutyl paraben on the expression of calbindin-D9k in GH3 rat pituitary cells

Estrogen Receptors are Involved in Xenoestrogen Induction of Growth Hormone in the Rat Pituitary Gland

The astroglial and stem cell functions of adult rat folliculostellate cells

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