To understand the interactive effects of O 3 and CO 2 on rice leaves; gas exchange, chlorophyll (Chl) fluorescence, ascorbic acid and glutathione were examined under acute (5 h), combined exposures of O 3 (0, 0.1, or 0.3 cm 3 m -3 , expressed as O 0 , O 0.1 , or O 0.3 , respectively), and CO 2 (400 or 800 cm 3 m -3 , expressed as C 400 or C 800 , respectively) in natural-light gas-exposure chambers. The net photosynthetic rate (P N ), maximum (F v /F m ) and operating (F q '/F m ') quantum efficiencies of photosystem II (PSII) in young (8 th ) leaves decreased during O 3 exposure. However, these were ameliorated by C 800 and fully recovered within 3 d in clean air (O 0 + C 400 ) except for the O 0.3 + C 400 plants. The maximum PSII efficiency at 1,500 μmol m -2 s -1 PPFD (F v '/F m ') for the O 0.3 + C 400 plants decreased for all measurement times, likely because leaves with severely inhibited P N also had a severely damaged PSII. The P N of the flag (16 th ) leaves at heading decreased under O 3 exposure, but the decline was smaller and the recovery was faster than that of the 8 th leaves. The F q '/F m ' of the flag leaves in the O 0.3 + C 400 and O 0.3 + C 800 plants decreased just after gas exposure, but the F v /F m was not affected. These effects indicate that elevated CO 2 interactively ameliorated the inhibition of photosynthesis induced by O 3 exposure. However, changes in antioxidant levels did not explain the above interaction.