A callus-derived regeneration and Agrobacterium-mediated gene transformation developed for bilberry, Vaccinium myrtillus

Song, Yanping; Luo, Wenbo; Wu, Ying; Li, X.; Albert, Nick W.; Zhang, Y.; Chen, Xiangtao; Lin‐Wang, Kui; Deng, Cecilia; Hu, Zhubing; Zheng, Bingsong; Wang, Xiaofei; Espley, Richard V.; Cui, Fuqiang

doi:10.1007/s11240-023-02527-1

Cited by 1 publication

(2 citation statements)

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“…In addition, plant regeneration via organogenesis or somatic embryogenesis from the callus has been widely reported, which provides an alternative to propagating elite plant species [18][19][20]. The callus is amenable to induction from various explant types in the Jerusalem artichoke, such as the tubers, leaves, and stem cuttings [14,17,21].…”

Section: Establishment Of In Vitro Propagation Systemmentioning

confidence: 99%

“…During the past few decades, in vitro culture techniques have been explored for Jerusalem artichokes for rapid clonal propagation [13,14], microtuber induction [15], germplasm cryopreservation [16], inulin production [15], seed dormancy breaking [6], and genetic transformation [17]. Generally, callus induction is an important step in the in vitro regeneration protocols of many plants [18][19][20]. In the case of Jerusalem artichokes, researchers have shown that calli could be readily induced from tubers, leaves, and stem cuttings on MS basal medium supplemented with different types of plant growth regulators (PGRs) used in different concentrations [14,17,21,22].…”

Section: Introductionmentioning

confidence: 99%

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Plant Regeneration via Organogenesis in Jerusalem Artichokes and Comparative Analysis of Endogenous Hormones and Antioxidant Enzymes in Typical and Atypical Shoots

Zhang,

Yin

et al. 2023

Plants

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The Jerusalem artichoke (Helianthus tuberosus) is a tuberous plant with considerable nutrient and bioactive compounds. The optimization of the in vitro clonal propagation protocol is critical for large-scale reproduction and biotechnological applications of Jerusalem artichoke production. In this work, in vitro plant regeneration from the stem nodes of the Jerusalem artichoke via direct organogenesis is presented. In the shoot induction stage, the stem segments produced more shoots with vigorous growth on MS medium containing 0.5 mg/L 6-benzylaminopurine (6-BA). The concentrations of 6-BA and gibberellic acid (GA3) were both optimized at 0.5 mg/L for shoot multiplication, and the combination of 0.05 mg/L indole-3-butyric acid (IBA) and 0.05 mg/L 1-naphthylacetic acid (NAA) was the most responsive for root induction, yielding the largest number of roots. The regenerated plantlets were successfully hardened at a 96% survival rate and vigorously grew in the field. The genetic stability of the regenerated plants was confirmed by flow cytometry and simple sequence repeat (SSR) analysis. However, 17.3% of shoots on the optimum shoot induction medium had withered leaves and excessive callus (atypical shoots), which greatly reduced the induction efficiency. Enzyme activity in the typical and atypical shoots was compared. The atypical shoots had significantly higher levels of endogenous indole-3-acetic acid (IAA) and abscisic acid (ABA), as well as increased activity of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD), whereas the content of 6-BA, zeatin (ZT), and GA3 was significantly reduced. The activity of the three enzymes was positively correlated with the content of IAA and ABA, while being negatively correlated with that of 6-BA, ZT, and GA3. The results suggest that the poor growth of the atypical shoots might be closely related to the significant accumulation of endogenous IAA and ABA, thus significantly increasing antioxidant enzyme activity.

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