A calmodulin-binding protein from<i>Arabidopsis</i>has an essential role in pollen germination

Golovkin, Maxim; Reddy, Anireddy S. N.

doi:10.1073/pnas.1734110100

Cited by 102 publications

(77 citation statements)

References 61 publications

(67 reference statements)

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“…CaM is a ubiquitous multifunctional calcium sensor in all eukaryotes that mediates calcium action by regulating the activity/function of many unrelated proteins. Calcium and CaM are known to play a crucial role in pollen germination and pollen tube growth (Golovkin and Reddy, 2003).…”

Section: Discussionmentioning

confidence: 99%

Identification of differentially expressed genes involved in early bolting of Angelica sinensis (Apiaceae)

Yu¹,

Ma²,

Ja³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Angelica sinensis is a highly valued medicinal herb, known as female ginseng that is widely cultivated in China. Although A. sinensis is in great demand due to its multiple medicinal and food applications, its early bolting rate (almost 40%) seriously affects crop quality. To better understand its flowering mechanism, cDNA-amplified RFLP analysis was employed to look for gene expression differences between flower bud and shoot apical meristem tissues. Sixty-four primer sets were used, with each primer set amplified to 60 transcriptderived fragments. Some transcript-derived fragments were expressed only in the flower bud. After cloning, sequencing and a homology search, 46 distinct sequences were obtained; 26 of these were found to have homologous sequences in databases. These included transcaffeoyl-CoA 3-O-methyltransferase, 1-deoxy-d-xylulose 5-phosphate reductoisomerase, 15-cis-zeta-carotene isomerase, isoamylase, and calmodulin-binding protein. These genes are closely related to pollen germination and pollen tube growth, terpenoid backbone biosynthesis, and other metabolic pathways. Confirmation of differential expression

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Section: Discussionmentioning

confidence: 99%

Identification of differentially expressed genes involved in early bolting of Angelica sinensis (Apiaceae)

Yu¹,

Ma²,

Ja³

et al. 2012

Genet. Mol. Res.

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“…In particular, two different CAMBPs characterized in Arabidopsis, ACA9 (Autoinhibited Ca 2? ATPase) (Schiøtt et al 2004) and NPG1 (No Pollen Germination1) (Golovkin and Reddy 2003), have been reported to be required for pollen germination and pollen tube growth. ACA9 gene is primarily expressed in pollen and mutations in this gene result in a semisterile phenotype, due to the functions played by this plasma membrane Ca 2?…”

Section: Discussionmentioning

confidence: 99%

A loss-of-function mutation in Calmodulin2 gene affects pollen germination in Arabidopsis thaliana

et al. 2010

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Calmodulin (CAM) is an ubiquitous calcium binding protein whose function is to translate the signals, perceived as calcium concentration variations, into the appropriate cellular responses. In Arabidopsis thaliana there are 4 CAM isoforms which are highly similar, encoded by 7 genes, and one possible explanation proposed for the evolutionary conservation of the CAM gene family is that the different genes have acquired different functions so that they play possibly overlapping but non-identical roles. Here we report the characterization of the Arabidopsis mutant cam2-2, identified among the lines of the gene-trapping collection EXOTIC because of a distorted segregation of kanamycin resistance. Phenotypic analysis showed that in normal growth conditions cam2-2 plants were indistinguishable from the wild type while genetic analysis showed a reduced transmission of the cam2-2 allele through the male gametophyte and in vitro pollen germination revealed a reduced level of germination in comparison with the wild type. These results provide genetic evidence of the involvement of a CAM gene in pollen germination and support the theory of functional diversification of the CAM gene family.

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“…5). Consistently up-regulated are general genes with known functions in pollen germination and tube growth, such as Vanguard1 (At2g47040; Jiang et al, 2005) and No Pollen Germination1 (At2g43040; Golovkin and Reddy, 2003;Fig. 5).…”

Section: Microarray Data Validationmentioning

confidence: 92%

Whole Genome Analysis of Gene Expression Reveals Coordinated Activation of Signaling and Metabolic Pathways during Pollen-Pistil Interactions in Arabidopsis

et al. 2011

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Plant reproduction depends on the concerted activation of many genes to ensure correct communication between pollen and pistil. Here, we queried the whole transcriptome of Arabidopsis (Arabidopsis thaliana) in order to identify genes with specific reproductive functions. We used the Affymetrix ATH1 whole genome array to profile wild-type unpollinated pistils and unfertilized ovules. By comparing the expression profile of pistils at 0.5, 3.5, and 8.0 h after pollination and applying a number of statistical and bioinformatics criteria, we found 1,373 genes differentially regulated during pollen-pistil interactions. Robust clustering analysis grouped these genes in 16 time-course clusters representing distinct patterns of regulation. Coregulation within each cluster suggests the presence of distinct genetic pathways, which might be under the control of specific transcriptional regulators. A total of 78% of the regulated genes were expressed initially in unpollinated pistil and/or ovules, 15% were initially detected in the pollen data sets as enriched or preferentially expressed, and 7% were induced upon pollination. Among those, we found a particular enrichment for unknown transcripts predicted to encode secreted proteins or representing signaling and cell wall-related proteins, which may function by remodeling the extracellular matrix or as extracellular signaling molecules. A strict regulatory control in various metabolic pathways suggests that fine-tuning of the biochemical and physiological cellular environment is crucial for reproductive success. Our study provides a unique and detailed temporal and spatial gene expression profile of in vivo pollen-pistil interactions, providing a framework to better understand the basis of the molecular mechanisms operating during the reproductive process in higher plants.

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A calmodulin-binding protein fromArabidopsishas an essential role in pollen germination

Cited by 102 publications

References 61 publications

Identification of differentially expressed genes involved in early bolting of Angelica sinensis (Apiaceae)

Identification of differentially expressed genes involved in early bolting of Angelica sinensis (Apiaceae)

A loss-of-function mutation in Calmodulin2 gene affects pollen germination in Arabidopsis thaliana

Whole Genome Analysis of Gene Expression Reveals Coordinated Activation of Signaling and Metabolic Pathways during Pollen-Pistil Interactions in Arabidopsis

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