A calmodulin inhibitor blocks morphogenesis in Candida albicans

Roy, B.Gupta

doi:10.1016/0378-1097(87)90263-1

“…This morphogenesis is thought to be a critical step in the pathogenesis of fungal infections [9]. Furthermore, the azoic derivatives, bifonazole, econazolc, miconazoie, clotrimazole and ketoconazole, were all found to inhibit calmoduiin in a dose-dependent manner, using calmodulin-dependent phosphodicstorase as an indicator for the calmoduiin activity [10].…”

Section: Discussionmentioning

confidence: 99%

Anticandidal activity of trifluoperazine

Krajewska-Kułak

¹

,

Niczyporuk

²

1995

Acad Dermatol Venereol

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Background Trifluoperazine, a potent calmodulin inhibitor, is used as an antipsychotic drug. Similar agents have inhibited the growth of yeasts.Methods Susceptibility of 101 Candida albicans strains from patients were tested against trifluoperazine (Trif), ketoconazole (Ktz), and the combination of Ktz with Trif, using Sabouraud's broth.Results The minimal inhibitory concentrations (MlC)s for 101 strains of C albicans were: Trif 65.8±2.1 /xg/ml, Ktz 27.9 ± 9.1 /xg/ml. The combination of Ktz and Trif in various ratios (1:1, 1:2. 2:1) was found to exert synergistic effect and the mean values of the combinations were: Ktz ± Trif 2.72 + 0.17, 2.5 ±0.18, 3.43 + 0.21 ixg/m\. These results were significantly different (P< 0.001) when compared with ketoconazole alone.Outcome Our findings indicate that trifluoperazine (calmodulin inhibitor) increased the antifungal activity of Ktz against C. albicans in vitro.

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“…The inositol phosphate pathway in C. Albicans is reported to exist. Roy and Datta (1987) showed that calmodulin inhibitor, trifluoperazine (TFP) inhibited yeast to germ tube formation of C. Albicans induced by N-acetyl glucosamine [32]. Trifluoperazine (TFP) is known to be a protein kinase-C inhibitor [33].…”

Section: Discussionmentioning

confidence: 99%

Acetylcholine Induces Yeast to Hyphal Form Transition in Candida albicans

Ali¹,

Karuppayil²

2018

Fungal Genom Biol

3

0

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Background: In Candida Albicans, yeast to hyphal form transition can be induced by serum, proline, glucose, and N-acetyl glucosamine. Acetylcholine is a neuromodulator which can stimulate both muscarinic and nicotinic acetylcholine receptors in humans. In this study, we are reporting that acetylcholine can induce yeast to hyphal form transition in C. Albicans. The adenylyl cyclase inhibitor, MDL 12, 330A inhibited this transition indicating the role of cAMP. Muscarinic receptors in C. Albicans did not report yet. We have reported that C. Albicans Rrp9 exhibits identity and similarity with the human muscarinic receptor M1. In humans, activation of muscarinic M1 receptor can produce cAMP through inositol phosphate pathway. The inositol phosphate pathway in C. Albicans is already known. We have carried out the local and global alignment sequences between the proteins of humans and C. Albicans which are involved in inositol phosphate pathway. We found considerable identities and similarities between them. Herein, we hypothesize that acetylcholine may activate Rrp9 which may lead to activation of inositol phosphate signalling pathway in C. Albicans. This study suggests that Rrp9 may have a potential role in yeast to hyphal form transition in C. Albicans.

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“…In recent studies, evidence has been obtained that calmodulin might be involved in the regulation of yeast-mycelial transition of C albicans. This morphogenesis is thought to be a critical step in the pathogenesis of fungal infections [9]. Furthermore, the azoic derivatives, bifonazole, econazolc, miconazoie, clotrimazole and ketoconazole, were all found to inhibit calmoduiin in a dose-dependent manner, using calmodulin-dependent phosphodicstorase as an indicator for the calmoduiin activity [10].…”

Section: Discussionmentioning

confidence: 99%

Anticandidal activity of trifluoperazine

Krajewska-Kułak

¹

,

Niczyporuk

²

1995

Journal of the European Academy of Dermatology and Venereology

1

0

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Background Trifluoperazine, a potent calmodulin inhibitor, is used as an antipsychotic drug. Similar agents have inhibited the growth of yeasts.Methods Susceptibility of 101 Candida albicans strains from patients were tested against trifluoperazine (Trif), ketoconazole (Ktz), and the combination of Ktz with Trif, using Sabouraud's broth.Results The minimal inhibitory concentrations (MlC)s for 101 strains of C albicans were: Trif 65.8±2.1 /xg/ml, Ktz 27.9 ± 9.1 /xg/ml. The combination of Ktz and Trif in various ratios (1:1, 1:2. 2:1) was found to exert synergistic effect and the mean values of the combinations were: Ktz ± Trif 2.72 + 0.17, 2.5 ±0.18, 3.43 + 0.21 ixg/m\. These results were significantly different (P< 0.001) when compared with ketoconazole alone.Outcome Our findings indicate that trifluoperazine (calmodulin inhibitor) increased the antifungal activity of Ktz against C. albicans in vitro.

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A calmodulin inhibitor blocks morphogenesis in Candida albicans

Cited by 6 publications

References 0 publications

Anticandidal activity of trifluoperazine

Anticandidal activity of trifluoperazine

Acetylcholine Induces Yeast to Hyphal Form Transition in Candida albicans

Anticandidal activity of trifluoperazine

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