A Candidate Antigen of the Recombinant Membrane Protein Derived from the Porcine Deltacoronavirus Synthetic Gene to Detect Seropositive Pigs

Castañeda-Montes, Francisco Jesus; Cerriteño-Sánchez, José Luis; Castañeda-Montes, María Azucena; Cuevas-Romero, Julieta Sandra; Mendoza-Elvira, Susana

doi:10.3390/v15051049

Cited by 2 publications

(1 citation statement)

References 77 publications

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“…In this study, sera samples from three of these key swine-producing states (Jalisco, Guanajuato, and Veracruz) were analyzed, suggesting a direct relationship between the seroprevalence of PEDV and the high capacity of production. Recently, Castañeda-Montes et al, 2023 [ 33 ] analyzed sera samples by an iELISA with a recombinant Porcine Deltacoronavirus (PDCoV) membrane protein. This study was conducted on sera from the central region of Mexico, which includes Jalisco and Guanajuato.…”

Section: Discussionmentioning

confidence: 99%

Seroepidemiology Study of Porcine Epidemic Diarrhea Virus in Mexico by Indirect Enzyme-Linked Immunosorbent Assay Based on a Recombinant Fragment of N-Terminus Domain Spike Protein

García-González,

Cerriteño-Sánchez,

Cuevas-Romero

et al. 2023

Microorganisms

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Porcine epidemic diarrhea (PED) is an intestinal disease caused by the porcine epidemic diarrhea virus (PEDV) and affects Mexico’s swine industry. Despite the disease initially being described in Mexico in 2013, there has been no research into the virus’s seroepidemiology carried out in Mexico. Thus, the goal of this study was to develop an indirect ELISA (iELISA) based on a recombinant N-terminal domain truncated spike (S) protein (rNTD-S) of PEDV to evaluate serum obtained from different pig-producing states in Mexico. A total of 1054 sera were collected from pig farms, slaughterhouses, and backyard production in the states of Aguascalientes, Guanajuato, Hidalgo, Jalisco, Morelos, Queretaro, Sinaloa, and Veracruz between 2019 and 2021. The rNTD-S protein was expressed in E. coli BL21 (DE3) cells. Negative and positive serum samples used in the iELISA were previously tested by Western blot. According to our findings, 61.66% of the serum samples (650/1054) were positive, with Jalisco having the highest percentage of positive samples, at a rate of 21.44% (226/1054). This is the first seroepidemiology study of PEDV carried out in Mexico, revealing that the virus is still circulating since the initial outbreak; furthermore, it provides an overview of PEDV’s spread and high level of persistence across the country’s key swine-producing states.

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Section: Discussionmentioning

confidence: 99%

Seroepidemiology Study of Porcine Epidemic Diarrhea Virus in Mexico by Indirect Enzyme-Linked Immunosorbent Assay Based on a Recombinant Fragment of N-Terminus Domain Spike Protein

García-González,

Cerriteño-Sánchez,

Cuevas-Romero

et al. 2023

Microorganisms

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Development and Application of an Indirect Enzyme‐Linked Immunosorbent Assay Based on a Recombinant Matrix Protein for the Serological Study of Porcine Deltacoronavirus in Mexican Pigs

Castañeda Montes,

Cerriteño Sánchez,

Cuevas‐Romero

et al. 2024

Veterinary Medicine & Sci

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Porcine deltacoronavirus (PDCoV) is an infectious disease that causes diarrhoea in pigs of different ages; however, piglets are more susceptible. PDCoV was first reported in 2012 in China and Hong Kong. Later, it was first reported in the USA in 2014 and in Mexico in 2019. Several studies have shown that M protein is highly conserved and, therefore, suitable for diagnostic systems. In this study, for the first time, an indirect enzyme‐linked immunosorbent assay (iELISA) based on a recombinant M protein (rM‐PDCoV) was developed to evaluate the seroprevalence of PDCoV in four states in Mexico. High sensitivity (83%) and specificity (100%) were observed for the iELISA. The kappa index calculated a nearly perfect agreement (0.8831) compared to the Western blot (gold standard test), suggesting acceptable statistical value support. In this study, 50.38% of the serum samples from backyard pigs were PDCoV‐positive. The serological comparison showed that PDCoV/PEDV coinfections occurred in 31.98% of the analysed sera. These results can enrich our understanding of how this virus spreads and enable the evaluation of PDCoV infections. Moreover, it highlights the importance of continually investigating the seroprevalence of PDCoV in Mexico because there is also no information about the current prevalence of the disease.

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A Candidate Antigen of the Recombinant Membrane Protein Derived from the Porcine Deltacoronavirus Synthetic Gene to Detect Seropositive Pigs

Cited by 2 publications

References 77 publications

Seroepidemiology Study of Porcine Epidemic Diarrhea Virus in Mexico by Indirect Enzyme-Linked Immunosorbent Assay Based on a Recombinant Fragment of N-Terminus Domain Spike Protein

Seroepidemiology Study of Porcine Epidemic Diarrhea Virus in Mexico by Indirect Enzyme-Linked Immunosorbent Assay Based on a Recombinant Fragment of N-Terminus Domain Spike Protein

Development and Application of an Indirect Enzyme‐Linked Immunosorbent Assay Based on a Recombinant Matrix Protein for the Serological Study of Porcine Deltacoronavirus in Mexican Pigs

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