A carbon nanocages-mediated fluorescent aptasensor for aflatoxin B1 detection based on T7 exonuclease double recycling amplification

Recent advances in nucleic acid signal amplification-based aptasensors for sensing mycotoxins

“…(5) To overcome the inherent amplification bias and non-specific amplification in NASAT, the introduction of nanomaterials (e.g., metal nanoparticles, carbon nanotubes, GO, and UCNPs) into amplification reactions may minimize the amplification bias and eliminate the non-specific amplification. 119,132,152,158,159 Compared with the nanosensors used directly for mycotoxin detection, the aptasensors based on nanomaterials and NASAT possess improved sensitivity, facilitating the ultra-sensitive monitoring of mycotoxins in complex food substrates from farm to table. (6) The NASAT-based aptasensors for mycotoxin detection reported above are largely limited to laboratory assays.…”

“…5E). 119 The CNC is introduced as the fluorescence resonance energy transfer (FRET) receptors to quench the fluorophore-labeled DNA probe (FAM-cDNA). In the presence of target AFB1, T7 Exo triggers a double recycling amplification reaction, inducing the digestion of a large number of FAM-cDNAs and the release of FAM fluorophores.…”

“…(D) The construction of an electrochemical aptasensor based on Exo III-powered WDNA for the DON assay 118. (E) The construction of a fluorescent aptasensor based on the T7 Exo double recycling amplification for the AFB1 assay 119.…”