A carbonic anhydrase pseudogene sensitizes selectBrucellalineages to low CO₂tension

Abstract

Brucella are intracellular pathogens that cause a disease known as brucellosis. Though the genus is highly monomorphic at the genetic level, species have animal host preferences and some defining physiologic characteristics. Of note is the requirement for increased CO2 tension to cultivate particular species, which confounded early efforts to isolate B. abortus from diseased cattle. Differences in the capacity of Brucella species to assimilate CO2 are determined by mutations in the carbonic anhydrase gene, bcaA. Ancestral single nucleotide insertions in bcaA have resulted in frameshifted pseudogenes in B. abortus and B. ovis lineages, which underlie their inability to grow under the low CO2 tension of a standard atmosphere. Incubation of wild-type B. ovis in air selects for mutations that "rescue" a functional bcaA reading frame, which enables growth under low CO2 and enhances growth rate in high CO2. Consistent with this result, we show that heterologous expression of functional E. coli carbonic anhydrases enables B. ovis growth in air. Growth of wild-type B. ovis is acutely sensitive to a reduction in CO2 tension, while frame-rescued B. ovis mutants are completely insensitive to CO2 shifts. Wild-type B. ovis initiates a gene expression program upon CO2 downshift that resembles the stringent response and results in activation of its virB type IV secretion system. Our study provides evidence that loss-of-function insertion mutations in bcaA sensitize the response of B. ovis and B. abortus to reduced CO2 tension relative to other Brucella lineages. CO2-dependent starvation and virulence gene expression programs in these species may influence persistence or transmission in natural hosts. ImportanceBrucella spp. are highly related, but exhibit differences in animal host preference that must be determined by genome sequence differences. B. ovis and the majority of B. abortus strains require increased CO2 tension to be cultivated in vitro, and harbor conserved insertional mutations in the carbonic anhydrase, bcaA, which underlie this trait. Mutants that grow in a standard atmosphere, first reported nearly a century ago, are easily selected in the laboratory. These mutants harbor varied indel polymorphisms in bcaA that restore its consensus reading frame and rescue its function. Loss of bcaA function has evolved independently in the B. ovis and B. abortus lineages, and results in a dramatically increased sensitivity to CO2 limitation.

Quantification ofBrucella abortuspopulation structure in a natural host

“…Given the success of the approach outlined here, one can envision using similar approaches for studying B. abortus transmission in suitably controlled field settings. We have also recently constructed a Brucella ovis bar-seq mutant library (55), which may be similarly useful for assessing the population biology of this ovine pathogen during infection/transmission.…”

Immunological pathways of macrophage response to Brucella ovis infection

“…It grows well in the solid culture of defibrinated amniotic blood and grows poorly in an ordinary medium. 11 – 13 The genome of B. ovis is about 3.29 Mb, including two chromosomes and 3200 open reading frames. The main routes of transmission of B. ovis are digestive tract, skin, mucous membrane, and reproductive tract.…”