A case of aconitine poisoning with analysis of aconitine alkaloids by GC/SIM

Yoshioka, Naofumi; Gonmori, Kunio; Tagashira, Akira; Boonhooi, Ong; Hayashi, Masato; Saitō, Yoshimi; Mizugaki, Michinao

doi:10.1016/s0379-0738(96)01980-9

Cited by 49 publications

(24 citation statements)

References 3 publications

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“…by eating plant material or by the uptake of Aconitum preparations. The suicidal and homicidal use of aconite tubers has also been reported [12,13,14,15,16,17,18,19,20,21].…”

Section: Introductionmentioning

confidence: 95%

“…This leads to severe vomiting, colicky diarrhoea, intense pain and then paralysis of the skeletal muscles. Following the onset of lifethreatening arrhythmia, including ventricular tachycardia and ventricular fibrillation, death finally occurs as a result of respiratory paralysis or cardiac arrest [2,12,13,14].…”

Section: Introductionmentioning

confidence: 99%

“…For the analysis of the Aconitum alkaloids in biological specimens such as blood, serum and urine, several GC-MS methods have been described [12,18,21,28,29]. These employ a variety of extraction procedures followed by derivatisation to their TMS derivatives.…”

Section: Introductionmentioning

confidence: 99%

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Determination of aconitine in body fluids by LC-MS-MS

et al. 2004

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A very sensitive and specific method was developed for the determination of aconitine, the main toxic alkaloid from plants of the genus Aconitum L., in biological samples. The method comprised solid-phase extraction using mixed-mode Cs cation exchange columns followed by liquid chromatography-tandem mass spectrometry (LC-MS-MS). Chromatographic separation was achieved with a RP8 column. Detection of aconitine was achieved using electrospray in the positive ionisation mode and quantification was performed using multiple reaction monitoring with m/z 646.4 as precursor ion, i.e. [M+H]+ of aconitine and m/z 586.5, m/z 526.4 and m/z 368.4 as product ions after collision-induced dissociation. The method was fully validated for the analysis of blood samples: the limit of detection and the limit of quantitation were 0.1 ng/g and 0.5 ng/g, respectively. Within the linear calibration range of 0.5-25 ng/g, analytical recovery was 79.9%. In two fatal cases with suspected aconite intoxication, aconitine could be detected in blood samples at concentrations of 10.0 and 12.1 ng/g. In one case, aconitine could also be detected in the stomach content (3 ng/g) and in the other in the urine (180 ng/ml).

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“…by eating plant material or by the uptake of Aconitum preparations. The suicidal and homicidal use of aconite tubers has also been reported [12,13,14,15,16,17,18,19,20,21].…”

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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Determination of aconitine in body fluids by LC-MS-MS

et al. 2004

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“…Generally, methods to identify toxic plants have involved morphological analysis, gas chromatography, and HPLC/ MS [13][14][15][16][17][18]; however, these methods are time consuming and are complicated for identification. A trace toxic sample is difficult to detect by morphological analysis.…”

Section: Introductionmentioning

confidence: 99%

Genus identification of toxic plant by real-time PCR

Matsuyama

Nishi

2010

Int J Legal Med

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Some plants have toxicities that are dangerous for humans. In the case of poisoning by toxic plants, a rapid and easy screening test is required for accurate medical treatment or forensic investigation. In this study, we designed specific primer pairs for identification of toxic plants, such as subgenus Aconitum, genus Ricinus, genus Illicium, and genus Scopolia, by internal transcribed spacer sequences of nuclear ribosomal DNA. Allied species of target plants, foods, and human DNA were not detected, but each primer pair provided a specific PCR product from the target plant using real-time PCR. This method can detect the subgenus Aconitum, genus Ricinus, and genus Scopolia with template DNA of 10 pg, respectively, and genus Illicium with 1 pg. Furthermore, each primer pair provided the exact PCR product from digested target plants in artificial gastric fluid. When a trace unknown plant sample in forensic investigation is collected from stomach contents, this PCR assay may be useful for screening toxic plants.

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“…Aconitine poisoning is most frequently caused by accidental ingestion, as it can be mistaken for an edible grass and by the use of herbal medicines, mainly in Asian countries where these are more used [7, 8, 23-25, 35, 40, 41]. A few fatal accidents have been reported [5,7,8,14,17,35,36,41], and as it is highly toxic, aconitine or Aconitum species are sometimes used in suicides and homicides [11-13, 20, 28, 36]. Even in ancient times it was known as a poison [29].…”

Section: Introductionmentioning

confidence: 99%

Aconitine involvement in an unusual homicide case

et al. 2006

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We describe a homicide complicated by an aconitine poisoning, which was initially thought to be a strangulation case. Routine toxicological analyses demonstrated only a small amount of alcohol in the blood and the urine. The case could not be clarified until 5 years after the event. A new element in the investigation made the wife the prime suspect, and finally, after thorough interrogation, she confessed her crime. She had mixed a decoction of three plants of Aconitum with red wine. Additional toxicological analyses, using the liquid chromatography-tandem mass spectrometry (LC-MS-MS) technique demonstrated 810 ng/ml of aconitine in urine, 6.5 ng/g in liver and 1.3 ng/g in the kidneys. Even though aconitine poisoning is still rare in Europe, it should be taken into account in suicides and homicides, particularly in unclarified cases.

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A case of aconitine poisoning with analysis of aconitine alkaloids by GC/SIM

Cited by 49 publications

References 3 publications

Determination of aconitine in body fluids by LC-MS-MS

Determination of aconitine in body fluids by LC-MS-MS

Genus identification of toxic plant by real-time PCR

Aconitine involvement in an unusual homicide case

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