A case of blaNDM-1-positive Salmonella Kottbus, Denmark, November 2020

Nielsen, Hans Linde; Thomsen, Philip Kjettinge; Litrup, Eva; Torpdahl, Mia; Overballe‐Petersen, Søren; Hansen, Frank; Christensen, Anne Kathrine Schultz; Hasman, Henrik

doi:10.2807/1560-7917.es.2021.26.26.2100569

Cited by 4 publications

(2 citation statements)

References 12 publications

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“…This time interval indicates the possibility of in vivo transmission from E. coli to C. koseri , highlighting the occurrence of interspecies plasmid transfer within the gut. A similar in vivo transfer was reported by H. L. Nielsen et al, where a bla NDM-1 -carrying IncN2 plasmid was identified in Salmonella Kottbus from the fecal sample of an individual, and was also found in E. coli and Citrobacter freundii in the same patient, suggesting a transfer between species ( Nielsen et al, 2021 ).…”

Section: Discussionsupporting

confidence: 80%

Co-colonization of different species harboring KPC or NDM carbapenemase in the same host gut: insight of resistance evolution by horizontal gene transfer

Ji,

Zhu,

Zhao

et al. 2024

Front. Microbiol.

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IntroductionThe dissemination of carbapenem-resistant Enterobacteriales (CRE) in nosocomial settings is primarily associated with the horizontal transfer of plasmids. However, limited research has focused on the in-host transferability of carbapenem resistance. In this study, ten isolates were collected from gut specimens of five individuals, each hosting two different species, including Escherichia coli, Klebsiella pneumoniae, Klebsiella aerogenes, Enterobacter cloacae, or Citrobacter koseri.MethodsSpecies identification and antimicrobial susceptibility were determined by MALDI-TOF MS and broth microdilution method. Carbapenemase genes were detected and localized using PCR, S1-PFGE and southern blot. The transferability of carbapenemase genes between species was investigated through filter mating experiments, and the genetic contexts of the plasmids were analyzed using whole genome sequencing.Results and discussionOur results revealed that each of the ten isolates harbored a carbapenemase gene, including blaNDM-5, blaNDM-1, or blaKPC-2, on a plasmid. Five different plasmids were successfully transferred to recipient cells of E. coli, K. pneumoniae or A. baumannii by transconjugation. The genetic contexts of the carbapenemase gene were remarkably similar between the two CRE isolates from each individual. This study highlights the potential for interspecies plasmid transmission in human gut, emphasizing the colonization of CRE as a significant risk factor for the dissemination of carbapenemase genes within the host. These findings underscore the need for appropriate intestinal CRE screening and colonization prevention.

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Section: Discussionsupporting

confidence: 80%

Co-colonization of different species harboring KPC or NDM carbapenemase in the same host gut: insight of resistance evolution by horizontal gene transfer

Ji,

Zhu,

Zhao

et al. 2024

Front. Microbiol.

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“…The carbapenemase genes identified were bla OXA‐48 (two isolates) and bla NDM‐1 . The latter finding is suggested to have occurred via interspecies plasmid transfer in an immunocompromised host as the bla NDM‐1 gene was also identified in E. coli and C. freundii on the same 43 kb IncN2 plasmid, isolated from the same patient (Nielsen et al, 2020 ).…”

Section: Discussionmentioning

confidence: 95%

The European Union Summary Report on Antimicrobial Resistance in zoonotic and indicator bacteria from humans, animals and food in 2019–2020

2022

EFS2

132

119

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Data on antimicrobial resistance (AMR) in zoonotic and indicator bacteria from humans, animals and food are collected annually by the EU Member States (MSs), jointly analysed by the EFSA and the ECDC and reported in a yearly EU Summary Report. The annual monitoring of AMR in animals and food within the EU is targeted at selected animal species corresponding to the reporting year. The 2020 monitoring specifically focussed on poultry and their derived carcases/meat, while the monitoring performed in 2019 specifically focused on fattening pigs and calves under 1 year of age, as well as their derived carcases/meat. Monitoring and reporting of AMR in 2019–2020 included data regarding Salmonella, Campylobacter and indicator E. coli isolates, as well as data obtained from the specific monitoring of presumptive ESBL‐/AmpC‐/carbapenemase‐producing E. coli isolates. Additionally, some MSs reported voluntary data on the occurrence of methicillin‐resistant Staphylococcus aureus in animals and food, with some countries also providing data on antimicrobial susceptibility. This report provides an overview of the main findings of the 2019–2020 harmonised AMR monitoring in the main food‐producing animal populations monitored, in carcase/meat samples and in humans. Where available, monitoring data obtained from pigs, calves, broilers, laying hens and turkeys, as well as from carcase/meat samples and humans were combined and compared at the EU level, with particular emphasis on multidrug resistance, complete susceptibility and combined resistance patterns to critically important antimicrobials, as well as Salmonella and E. coli isolates possessing ESBL‐/AmpC‐/carbapenemase phenotypes. The key outcome indicators for AMR in food‐producing animals, such as complete susceptibility to the harmonised panel of antimicrobials in E. coli and the prevalence of ESBL‐/AmpC‐producing E. coli have been specifically analysed over the period 2014–2020.

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Co-existence of two ciprofloxacin-resistant Salmonella enterica serovar Kentucky strains in the urine of a bladder cancer patient

Xu¹,

Cao²,

Zhang³

et al. 2022

Journal of Global Antimicrobial Resistance

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A case of blaNDM-1-positive Salmonella Kottbus, Denmark, November 2020

Cited by 4 publications

References 12 publications

Co-colonization of different species harboring KPC or NDM carbapenemase in the same host gut: insight of resistance evolution by horizontal gene transfer

Co-colonization of different species harboring KPC or NDM carbapenemase in the same host gut: insight of resistance evolution by horizontal gene transfer

The European Union Summary Report on Antimicrobial Resistance in zoonotic and indicator bacteria from humans, animals and food in 2019–2020

Co-existence of two ciprofloxacin-resistant Salmonella enterica serovar Kentucky strains in the urine of a bladder cancer patient

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