A Case of Misidentification ofAspergillus versicolorComplex asScopulariopsisSpecies Isolated from a Homograft

Abstract

We report a case of the isolation of the Aspergillus versicolor complex, initially misidentified by morphological characteristics as the Scopulariopsis species, from a homograft with a bicuspidalized pulmonary valve. An eighteen-month-old female, who had critical pulmonary stenosis, underwent pulmonary valve replacement. On postoperative day 8, she developed a fever, which did not respond to empiric broad-spectrum antibiotics. While no definitive source was identified, a filamentous fungus was isolated from the thawed homograft tissue culture prior to implantation on the operation day. The colonies were powdery green with white edges on Sabouraud dextrose agar. Microscopic examination showed septate hyphae with branched conidiophores and chains of spiny conidia, which suggested Scopulariopsis species. After direct sequencing of the internal transcribed spacer (ITS) regions, the fungus was identified as the A. versicolor complex. To our knowledge, the isolation of the A. versicolor complex from a homograft valve has not been previously described. This case shows that laboratory staff should be aware that microscopic morphology of the A. versicolor complex can resemble that of a number of other genera, including Scopulariopsis species. (Ann Clin Microbiol 2013;16: 105-109)

Misidentification ofCandida guilliermondiiasC. famataamong Strains Isolated from Blood Cultures by the VITEK 2 System

“…The authors of that paper asserted the importance of this problem because of differences in antifungal susceptibility [ 3 ], especially the fact that the resistance rate to fluconazole and amphotericin B is high in C. guilliermondii . Similar misidentification can be seen in the identification of mold-form fungi [ 19 ]. We agree with their recommendation concerning the importance of using many molecular techniques for diagnosis of infectious diseases to overcome the limits of conventional methods [ 20 , 21 ].…”

Keanekaragaman Kapang Patogen dan Non Patogen pada Imago, Kokon, dan Larva Instar Keenam Ulat Sutera Liar Attacus atlas (Lepidoptera: Saturniidae)

“…Struktur gerigi dari konidia terlihat pada gambaran mikroskopis, namun kurang begitu jelas. Hal ini sesuai dengan Hong et al, (2007) dan Huh et al, (2013), bahwa koloni A. versicolor tumbuh dengan cepat dan memiliki diameter 21-23 mm dalam seminggu. Menurut Hong et al,(2007) diperlukan scanning electron microscope (SEM) untuk melihat struktur dan morfologi dari konidia yang bergerigi dari A. versicolor dengan lebih jelas.…”

Evaluation of Industrial Biocides on a Novel Aspergillus versicolor TANCK-1 and Elucidation of Their Probable Biocidal Mechanism

“…(Fig. 2) but culture appeared as Aspergillus sp., with conidia arising from biseriated phialides [25,26]. Its occurrence is so uncommon in leather industry; the strain was initially mistaken for a Penicillium sp., which prompted us to go for genotypic identification.…”