2012
DOI: 10.1016/j.biologicals.2012.05.001
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A case of mistaken identity? Vaccinia virus in a live camelpox vaccine

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Cited by 6 publications
(14 citation statements)
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“…We selected three genes to represent different regions on the CMLV genome; the ATIP gene region which, was used to determine the species of the OPXV via PCR by producing amplicons of different sizes ( Meyer et al , 1994 ) or VACV similar sequence ( Yousif and Al Ali, 2012 ), second, we targeted the L1R gene IMV gene of ORXV also used by Yousif and Al Ali (2012) , additionally, a unique region in the CMLV genome which encode open reading frame (ORF) 185 claimed absent in other OPXVs ( Afonso et al , 2002 ) was used.…”
Section: Methodsmentioning
confidence: 99%
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“…We selected three genes to represent different regions on the CMLV genome; the ATIP gene region which, was used to determine the species of the OPXV via PCR by producing amplicons of different sizes ( Meyer et al , 1994 ) or VACV similar sequence ( Yousif and Al Ali, 2012 ), second, we targeted the L1R gene IMV gene of ORXV also used by Yousif and Al Ali (2012) , additionally, a unique region in the CMLV genome which encode open reading frame (ORF) 185 claimed absent in other OPXVs ( Afonso et al , 2002 ) was used.…”
Section: Methodsmentioning
confidence: 99%
“…To corroborate the ATIP gene results, we amplified two more CMLV genes. First, IMV gene (L1R) was followed as previously described ( Yousif and Al Ali, 2012 ). Second, a unique region in the CMLV genome which encode ORF 185 absent in other OPXVs ( Afonso et al , 2002 ) was used for PCR primer design, namely 185F forward sequence 5’ CTCAATGAGAGTTCCTGACCATCC 3’ and 185R reverse 5’ ACACTCTAATACAACACAGGCAACA 3’ that amplify a 923 bp product.…”
Section: Methodsmentioning
confidence: 99%
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“…Ducapox live attenuated vaccine is manufactured in South Africa, T8 killed vaccine in Morocco, and the Jouf-78 has been used to produce live attenuated vaccine in Saudi Arabia (9). Recently, it was discovered that the Jouf-78 vaccine contained a vaccinia virus (VACV) rather than CMLV (13); consequently, the production of this vaccine has ceased.…”
Section: Introductionmentioning
confidence: 99%