A Case of Suppressed Lu^a and Lu^b Antigens

The number of Lu^b antigen sites on the human erythrocyte membrane, as recognised by the murine monoclonal antibody BRIC-108, has been determined. The number of antibody molecules bound per cell on one example of cells of the phenotype Lu(a-b-) with recessive inheritance was an average of around 200 in replicate determinations, which probably represents non-specific antibody binding. A similar number of antibody molecules to this bound to trypsin- or pronase-treated normal cells or cells of the Lu(a-b-) phenotype associated with the inheritance of the X-borne gene, XS2. The number of binding sites on three examples of cells of the phenotype Lu(a-b-) with dominant inheritance was from 440 to 690. A variation in the number of binding sites per cell from 1,640 to 4,070 was found in five individuals with the phenotype Lu(a-b+) and from 850 to 1,820 in four individuals with the phenotype Lu(a+b+). Four individuals with the Lu(a+b-) phenotype had an average of 480 binding sites per cell. The Lu^b antigen therefore appears to a have low-site density and a variable level of expression on the erythrocyte surface.

Section: Methodscontrasting

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Estimation of the Number of Binding Sites for a Murine Monoclonal Anti-Lu^b on Human Erythrocytes

Merry¹,

Gardner²,

Parsons³

et al. 1987

“…The following counts were made from the publications [1-3, 5, 8, 9] or from further informa tion in the records of the MRC Blood Group Unit. The figures in brackets are counts from the family of Tippett [8], in which the dominance of the inhibitor was very probable, rather than certain. No samples of saliva were collected from any of the families, but the Lewis groups of the red cells served to show one recombination between In(Lu) and secretor; thus, here again, useful linkage is perhaps unlikely.…”

Section: The Family Larmentioning

confidence: 99%

“…Several more such families have been reported since [1,3,5,8,9, review 4]. Families were later described in which the phenotype Lu(a-b-) was clearly a recessive character, but the present paper deals only with the dominant form.…”

mentioning

confidence: 95%

See 1 more Smart Citation

The Genetics of a Dominant Inhibitor of the Lutheran Antigens

Taliano

Guévin

Tippett³

1973

Self Cite

Estimation of the Number of Binding Sites for a Murine Monoclonal Anti‐Lu^b on Human Erythrocytes

Merry¹,

Gardner

Parsons³

et al. 1987

The number of Lub antigen sites on the human erythrocyte membrane, as recognised by the murine monoclonal antibody BRIC-108, has been determined. The number of antibody molecules bound per cell on one example of cells of the phenotype Lu(a-b-) with recessive inheritance was an average of around 200 in replicate determinations, which probably represents non-specific antibody binding. A similar number of antibody molecules to this bound to trypsin- or pronase-treated normal cells or cells of the Lu(a-b-) phenotype associated with the inheritance of the X-borne gene, XS2. The number of binding sites on three examples of cells of the phenotype Lu(a-b-) with dominant inheritance was from 440 to 690. A variation in the number of binding sites per cell from 1,640 to 4,070 was found in five individuals with the phenotype Lu(a-b+) and from 850 to 1,820 in four individuals with the phenotype Lu(a+b+). Four individuals with the Lu(a+b-) phenotype had an average of 480 binding sites per cell. The Lub antigen therefore appears to a have low-site density and a variable level of expression on the erythrocyte surface.