Video clip is available online.Cases of thoracoscopic medial-basal segment (S7) segmentectomy are rarely reported, 1-3 possibly because S7 is the smallest 2 and deepest segment of the lungs. The advent of computed tomography screening has increased the detection of ground-glass opacity (GGO) nodules, 4 which are commonly found in S7. Currently, only 2 publications 1,2 have described the technical details of 3-port or 4-port thoracoscopic S7 segmentectomy, and there is still no specific treatment for some anatomic variations of S7. We introduce step by step our uniportal thoracoscopic S7 segmentectomy and S7b sub-segmentectomy, and discuss the surgical treatment principles for all S7 variants.