Vacuum tube well dewatering technology has gradually become an effective means to deal with low-permeability soil groundwater. The vacuum degree transfer law is an important element in the study of the mechanism of deep foundation pit vacuum tube well dewatering. To further study the transfer law of vacuum degree in vacuum tube well dewatering, a laboratory test setup for vacuum tube well dewatering was developed, and vacuum tube well dewatering tests were conducted under various in-well vacuum values, tube diameter, depth of tube well, and other factors. The variation law of vacuum degree under various working condition coupling was thoroughly analyzed in this research. The results show that with the increase of vacuum value in the well, the radial vacuum degree and vertical vacuum degree have about 14.9~47.7% and 14.9~32.4% increase, respectively, and transmission attenuation of radial vacuum degree, transmission attenuation of vertical vacuum degree, and the range of the vacuum field all increase to some extent. With the outward transmission of the vacuum degree, the transmission attenuation of the radial vacuum degree decreases rapidly and then tends to be stable, and the transmission attenuation of the vertical vacuum degree decreases gradually. The radial vacuum degree, the transmission attenuation of the radial vacuum degree, and the range of the radial vacuum field are not affected by the buried depth of the tube well. The study’s conclusions can serve as a foundation and point of reference for projects involving the design and implementation of deep foundation pit vacuum tube well dewatering.