A Casein Kinase II Phosphorylation Site in AtYY1 Affects Its Activity, Stability, and Function in the ABA Response

Wu, Xiuyun; Li, Tian

doi:10.3389/fpls.2017.00323

Cited by 13 publications

(13 citation statements)

References 26 publications

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“…However, M1 also exhibited casein kinase II phosphorylation and N-myristoylation-related activities. The former plays an important role in various environmental stresses and signaling by regulating protein activity and stability in various signaling pathways . The latter regulates plant responses against diverse stresses via membrane targeting and signal transduction .…”

Section: Resultsmentioning

confidence: 99%

Molecular Characterization of Germin-like Protein Genes in Zea mays (ZmGLPs) Using Various In Silico Approaches

Ilyas¹,

Ali

Binjawhar

et al. 2023

ACS Omega

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Germin (GER) and germin-like proteins (GLPs) play an important role in various plant processes. Zea mays contains 26 germin-like protein genes ( ZmGLPs ) located on chromosomes 2, 4, and 10; most of which are functionally unexplored. The present study aimed to characterize all ZmGLPs using the latest computational tools. All of them were studied at a physicochemical, subcellular, structural, and functional level, and their expression was predicted in plant development, against biotic and abiotic stresses using various in silico approaches. Overall, ZmGLPs showed greater similarity in their physicochemical properties, domain architecture, and structure, mostly localized in the cytoplasmic or extracellular regions. Phylogenetically, they have a narrow genetic background with a recent history of gene duplication events on chromosome 4. Functional analysis revealed novel enzymatic activities of phosphoglycolate phosphatase, adenosylhomocysteinase, phosphoglycolate phosphatase-like, osmotin/thaumatin-like, and acetohydroxy acid isomeroreductase largely mediated by disulfide bonding. Expression analysis revealed their crucial role in the root, root tips, crown root, elongation and maturation zones, radicle, and cortex with the highest expression being observed during germination and at the maturity levels. Further, ZmGLPs showed strong expression against biotic ( Aspergillus flavus , Colletotrichum graminicola , Cercospora zeina , Fusarium verticillioides , and Fusarium virguliforme ) while limited expression was noted against abiotic stresses. Concisely, our results provide a platform for additional functional exploration of the ZmGLP genes against various environmental stresses.

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Section: Resultsmentioning

confidence: 99%

Molecular Characterization of Germin-like Protein Genes in Zea mays (ZmGLPs) Using Various In Silico Approaches

Ilyas¹,

Ali

Binjawhar

et al. 2023

ACS Omega

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“…The yeast two‐hybrid assay was carried out as described previously (Wu and Li, ). The TaDA1‐A and TaGW2‐B CDSs were amplified and sub‐cloned into the pGBKT7 and pGADT7 vectors, respectively, to generate the TaDA1‐A‐BD and TaGW2‐B‐AD plasmids.…”

Section: Methodsmentioning

confidence: 99%

TaDA1, a conserved negative regulator of kernel size, has an additive effect with TaGW2 in common wheat (Triticum aestivum L.)

Hao

et al. 2019

Plant Biotechnology Journal

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104

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Summary Kernel size is an important trait determining cereal yields. In this study, we cloned and characterized TaDA1, a conserved negative regulator of kernel size in wheat (Triticum aestivum). The overexpression of TaDA1 decreased the size and weight of wheat kernels, while its down‐regulation using RNA interference (RNAi) had the opposite effect. Three TaDA1‐A haplotypes were identified in Chinese wheat core collections, and a haplotype association analysis showed that TaDA1‐A‐HapI was significantly correlated with the production of larger kernels and higher kernel weights in modern Chinese cultivars. The haplotype effect resulted from a difference in TaDA1‐A expression levels between genotypes, with TaDA1‐A‐HapI resulting in lower TaDA1‐A expression levels. This favourable haplotype was found having been positively selected during wheat breeding over the last century. Furthermore, we demonstrated that TaDA1‐A physically interacts with TaGW2‐B. The additive effects of TaDA1‐A and TaGW2‐B on kernel weight were confirmed not only by the phenotypic enhancement arising from the simultaneous down‐regulation of TaDA1 and TaGW2 expression, but also by the combinational haplotype effects estimated from multi‐environment field data from 348 wheat cultivars. A comparative proteome analysis of developing transgenic and wild‐type grains indicated that TaDA1 and TaGW2 are involved in partially overlapping but relatively independent protein regulatory networks. Thus, we have identified an important gene controlling kernel size in wheat and determined its interaction with other genes regulating kernel weight, which could have beneficial applications in wheat breeding.

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“…Protein phosphatase 2C (PP2C) is a branch of protein phosphatase (Protein phosphatase, PP), encoded by PP2C gene family which is one of the largest gene families in plants ( Xue et al, 2008 ). Phosphorylation and dephosphorylation of regulatory proteins and enzymes are general mechanisms to transmit signals from the extracellular environment to the interior of the cell ( Xiu-Yun and Tian, 2017 ). Protein phosphatases counteract the action of the protein kinases, catalyze the dephosphorylation of phosphorylated protein molecules, play an important role in the modulation and reversibility of the phosphoregulatory mechanism ( Rodriguez, 1998 ).…”

Section: Introductionmentioning

confidence: 99%

Functional Study of BpPP2C1 Revealed Its Role in Salt Stress in Betula platyphylla

Xing

Zhang

et al. 2021

Front. Plant Sci.

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PP2C protein phosphatase family is one of the largest gene families in the plant genome. Many PP2C family members are involved in the regulation of abiotic stress. We found that BpPP2C1 gene has highly up-regulated in root under salt stress in Betula platyphylla. Thus, transgenic plants of Betula platyphylla with overexpression and knockout of BpPP2C1 gene were generated using a zygote transformation system. Under NaCl stress treatment, we measured the phenotypic traits of transgenic plants, chlorophyll-fluorescence parameters, peroxidase (POD) activity, superoxide dismutase (SOD) activity, and malondialdehyde (MDA) content. We found that BpPP2C1 overexpressed lines showed obvious salt tolerance, while BpPP2C1 knocked out plants were sensitive to salt stress. Transcriptome analysis identified significantly amount of differentially expressed genes associated with salt stress in BpPP2C1 transgenic lines, especially genes in abscisic acid signaling pathway, flavonoid biosynthetic pathway, oxidative stress and anion transport. Functional study of BpPP2C1 in Betula platyphylla revealed its role in salt stress.

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A Casein Kinase II Phosphorylation Site in AtYY1 Affects Its Activity, Stability, and Function in the ABA Response

Cited by 13 publications

References 26 publications

Molecular Characterization of Germin-like Protein Genes in Zea mays (ZmGLPs) Using Various In Silico Approaches

Molecular Characterization of Germin-like Protein Genes in Zea mays (ZmGLPs) Using Various In Silico Approaches

TaDA1, a conserved negative regulator of kernel size, has an additive effect with TaGW2 in common wheat (Triticum aestivum L.)

Functional Study of BpPP2C1 Revealed Its Role in Salt Stress in Betula platyphylla

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