A Catalogue of Molecular, Physiological and Symbiotic Properties of Soybean-Nodulating Rhizobial Strains from Different Soybean Cropping Areas of China

Thomas‐Oates, Jane; Bereszczak, J.; Edwards, Emma; Gill, Andrew A.; Noreen, Sadaf; Zhou, Jun; Chen, M.Z.; Miao, Lihong; Xie, Fu Li; Yang, Jiangke; Zhou, Qing; Yang, Shuran; Li, Xing; Wang, L.; Spaink, Herman P.; Schlaman, Helmi R. M.; Harteveld, Marga; Diaz, C. L.; Brussel, A. A. N. Van; Camacho, M. E.; Rodríguez-Navarro, Dulce N.; Santamarı́a, C.; Temprano, F.; Acebes, J.M.; Bellogín, Ramón A.; Buendía-Clavería, Ana M.; Cubo, María Teresa; Espuny, M. Rosario; Gil, Antonio; Gutiérrez, Rocío; Hidalgo, Ángeles; López‐Baena, Francisco Javier; Madinabeitia, Nuria; Medina, Carlos; Ollero, Francisco Javier; Vinardell, José‐María; Ruíz-Sainz, José E.

doi:10.1078/072320203322497491

Cited by 21 publications

(19 citation statements)

References 48 publications

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“…In the presence of SDS, silver-stained gels, in which the Alcian Blue treatment was omitted, showed the typical LPS ladder of bands that is observed in HH103 Rif R and other S. fredii strains (Parada et al 2006;Thomas-Oates et al 2003). The LPS electrophoretic profile of mutants SVQ536, SVQ537, SVQ538, and the wild-type strain HH103 Rif R were indistinguishable (Fig.…”

Section: Resultsmentioning

confidence: 90%

Sinorhizobium fredii HH103 does not strictly require KPS and/or EPS to nodulate Glycyrrhiza uralensis, an indeterminate nodule-forming legume

et al. 2011

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The Sinorhizobium fredii HH103 rkp-1 region, which is involved in capsular polysaccharide (KPS) biosynthesis, is constituted by the rkpU, rkpAGHIJ, and kpsF3 genes. Two mutants in this region affecting the rkpA (SVQ536) and rkpI (SVQ538) genes were constructed. Polyacrylamide gel electrophoresis and (1)H-NMR analyses did not detect KPS in these mutants. RT-PCR experiments indicated that, most probably, the rkpAGHI genes are cotranscribed. Glycine max cultivars (cvs.) Williams and Peking inoculated with mutants SVQ536 and SVQ538 showed reduced nodulation and symptoms of nitrogen starvation. Many pseudonodules were also formed on the American cv. Williams but not on the Asiatic cv. Peking, suggesting that in the determinate nodule-forming S. fredii-soybean symbiosis, bacterial KPS might be involved in determining cultivar-strain specificity. S. fredii HH103 mutants unable to produce KPS or exopolysaccharide (EPS) also showed reduced symbiotic capacity with Glycyrrhiza uralensis, an indeterminate nodule-forming legume. A HH103 exoA-rkpH double mutant unable to produce KPS and EPS was still able to form some nitrogen-fixing nodules on G. uralensis. Thus, here we describe for the first time a Sinorhizobium mutant strain, which produces neither KPS nor EPS is able to induce the formation of functional nodules in an indeterminate nodule-forming legume.

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Section: Resultsmentioning

confidence: 90%

Sinorhizobium fredii HH103 does not strictly require KPS and/or EPS to nodulate Glycyrrhiza uralensis, an indeterminate nodule-forming legume

et al. 2011

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“…The studies on rhizobial diversity often lack quantitative information on N fixation efficiency. Most of the soybean rhizobia isolated by Thomas-Oates et al (2003) were classified as N fixing with varying level of efficiency. As the N fixation efficiency of natural soil population may be assumed , and glnII (c) gene sequences showing the relationships between selected soybean isolates (in boldface) and reference rhizobia.…”

Section: Discussionmentioning

confidence: 99%

Symbiotic matching, taxonomic position, and field assessment of symbiotically efficient rhizobia isolated from soybean root nodules in Sichuan, China

et al. 2015

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To find wide host range compatible potential inoculant strains with high efficiency in N fixation, we isolated rhizobia from soybean root nodules in Sichuan. Most of the isolated rhizobia were no-or low-effective strains. Eight out of 75 isolates promoted significantly the growth of soybean cultivar Gongxuan No. 1. When tested with three more soybean cultivars, the isolates assigned as Bradyrhizobium japonicum SCAUs36, Bradyrhizobium diazoefficiens SCAUs46, and Ensifer fredii SCAUs65 promoted significantly (P<0.05) the growth of each cultivar. The nodC and nifH genes of bradyrhizobial SCAUs36 and SCAUd46 and E. fredii SCAUs65 grouped together with those of broad host range strains. In field experiments with two more soybean cultivars, B. diazoefficiens SCAUs46 and E. fredii SCAUs65 performed well both in mild and hot-dry climates, implying that the isolates were potential inoculants to be applied in diverse soil and climate conditions.

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“…Thus, these rhizobial strains exhibit marked soybean cultivar specificity. Years later, the isolation of new fast-growing soybean rhizobia strains that were able to form nitrogen-fixing nodules with both Asiatic and American soybean cultivars was reported Dowdle and Bohlool, 1985;Rodríguez-Navarro et al, 1996;Thomas-Oates et al, 2003;Yang et al, 2001). Although most of the fast-growing soybean rhizobia strains have been isolated from Chinese soils, isolations from other geographical regions have also been reported (Hernandez and Focht, 1984;Young et al, 1988).…”

Section: The Soybean Rhizobia Group Consisting Of Fast and Slow Growimentioning

confidence: 90%

“…The chemical structure of Nod factors produced by different S. fredii strains, including HH103, has been reported (Bec-Ferte et al, 1994;Gil-Serrano et al, 1997;Thomas-Oates et al, 2003). This structure consist of a backbone of three to five ␤-(1→4)-linked N-acetyl-glucosamine (GlcNAc) residues bearing an amide bound fatty acyl residue, saturated or unsaturated, on the non-reducing terminal GlcNAc residue.…”

Section: Nodulation Factorsmentioning

confidence: 99%

Symbiotic properties and first analyses of the genomic sequence of the fast growing model strain Sinorhizobium fredii HH103 nodulating soybean

Margaret

Becker

Blom

et al. 2011

Journal of Biotechnology

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A Catalogue of Molecular, Physiological and Symbiotic Properties of Soybean-Nodulating Rhizobial Strains from Different Soybean Cropping Areas of China

Cited by 21 publications

References 48 publications

Sinorhizobium fredii HH103 does not strictly require KPS and/or EPS to nodulate Glycyrrhiza uralensis, an indeterminate nodule-forming legume

Sinorhizobium fredii HH103 does not strictly require KPS and/or EPS to nodulate Glycyrrhiza uralensis, an indeterminate nodule-forming legume

Symbiotic matching, taxonomic position, and field assessment of symbiotically efficient rhizobia isolated from soybean root nodules in Sichuan, China

Symbiotic properties and first analyses of the genomic sequence of the fast growing model strain Sinorhizobium fredii HH103 nodulating soybean

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