A catch-and-release nano-based gene delivery system

Franck, Christoph O.; Bistrovic Popov, Andrea; Ahmed, Ishtiaq; Hewitt, Rachel E.; Franslau, Luise; Tyagi, Puneet; Fruk, Ljiljana

doi:10.1039/d3nh00269a

Cited by 4 publications

(1 citation statement)

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“…2b). 34 The presence of a sizeable ionizable imidazole fraction of poly- l -histidine promotes proton buffering inside the internal endocytic body, which in turn leads to their rupture through the proton sponge effect. 35 By adjusting the ratio of pHis to pArg and the ratio of NPs to pDNA, comparable transfection efficiencies of Lipofectamine could be monitored by utilizing self-reported EGFP-pDNA.…”

Section: Strategies To Improve Gene Transfection Efficiencymentioning

confidence: 99%

Recent developments of polymeric delivery systems in gene therapeutics

Li,

Tian,

et al. 2024

Polym. Chem.

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Section: Strategies To Improve Gene Transfection Efficiencymentioning

confidence: 99%

Recent developments of polymeric delivery systems in gene therapeutics

Li,

Tian,

et al. 2024

Polym. Chem.

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Photoacoustic polydopamine-indocyanine green (PDA-ICG) nanoprobe for detection of senescent cells

Hartono,

Baker,

Else

et al. 2024

Sci Rep

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Cellular senescence is considered an important tumour suppression mechanism in response to damage and oncogenic stress in early lesions. However, when senescent cells are not immune-cleared and persist in the tumour microenvironment, they can drive a variety of tumour-promoting activities, including cancer initiation, progression, and metastasis. Additionally, there is compelling evidence demonstrating a direct connection between chemo(radio)therapy-induced senescence and the development of drug resistance and cancer recurrence. Therefore, detection of senescent cells in tissues holds great promise for predicting cancer occurrence earlier, assessing tumour progression, aiding patient stratification and prognosis, and informing about the efficacy of potential senotherapies. However, effective detection of senescent cells is limited by lack of biomarkers and readout strategies suitable for in vivo clinical imaging. To this end, a nanoprobe composed of biocompatible polydopamine (PDA) nanoparticle doped with FDA-approved indocyanine green (ICG) dye, namely PDA-ICG, was designed as a contrast agent for senescence detection using photoacoustic imaging (PAI). In an in vitro model of chemotherapy-induced senescence, PDA-ICG nanoprobe showed an elevated uptake in senescent cells relative to cancer cells. In addition to its improved photostability, 2.5-fold enhancement in photoacoustic signal relative to ICG was observed. Collectively, the results indicate that the PDA-ICG nanoprobe has the potential to be used as a contrast agent for senescence detection of chemotherapy-induced senescence using PAI.

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