A CCD-based image recording system for the CTEM

Roberts, P.; Chapman, J. N.; MacLeod, A M

doi:10.1016/0304-3991(82)90061-4

Cited by 48 publications

(17 citation statements)

References 10 publications

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“…However, due to radiation damage and signal saturation (Roberts, et al, 1982), CCDs cannot be placed in the direct path of the electron beam. A phosphorescent scintillation screen is required to convert the electron image to a photonic image that can be relayed to the CCD camera, either by fiber optics or a lens system.…”

Section: Introductionmentioning

confidence: 99%

Applications of direct detection device in transmission electron microscopy

Liang

Milazzo

Kleinfelder

et al. 2008

Journal of Structural Biology

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A prototype Direct Detection Device (DDD) camera system has shown great promise in improving both the spatial resolution and the signal to noise ratio for electron microscopy at 120-400 keV beam energies (Xuong, et al., 2007. Methods in Cell Biology, 79, 721-739). Without the need for a resolution-limiting scintillation screen as in the charge coupled device (CCD), the DDD camera can outperform CCD based systems in terms of spatial resolution, due to its small pixel size (5 μm). In this paper, the modulation transfer function (MTF) of the DDD prototype is measured and compared with the specifications of commercial scientific CCD camera systems. Combining the fast speed of the DDD with image mosaic techniques, fast wide-area imaging is now possible. In this paper, the first large area mosaic image and the first tomography dataset from the DDD camera are presented, along with an image processing algorithm to correct the specimen drift utilizing the fast readout of the DDD system.

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Section: Introductionmentioning

confidence: 99%

Applications of direct detection device in transmission electron microscopy

Liang

Milazzo

Kleinfelder

et al. 2008

Journal of Structural Biology

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“…Since then, digital imaging for the TEM has progressed significantly (Roberts et al, 1982). Many digital imaging systems have been produced between 1982 and the present (Aikens et al, 1989;Chapman et al, 1989;Daberkow et al, 1996;Downing and Hendrickson, 1999;Ellisman, 1993, 2000;Herrmann and Sikeler, 1995;Krivanek et al, 1991;Kujawa and Krahl, 1992;Spence and Zuo, 1998).…”

Section: Motivation For This Studymentioning

confidence: 98%

Transform-based backprojection for volume reconstruction of large format electron microscope tilt series

Lawrence

Bouwer

Perkins

et al. 2006

Journal of Structural Biology

102

116

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“…The signal-to-noise ratio, dened as the average pulse height of the photoelectron peak divided by the width 9 of the background peak, is equal to 20, 14, 10, 5 for, respectively, the local maximum pixel and the three-, ve-and nine-pixel clusters (Fig. 3a-d).…”

Section: Single-photoelectron Detectionmentioning

confidence: 99%

“…Because no \isolation" condition is imposed, it accepts also clusters due to more than one coalescent photoelectrons. The total pulse height o f a 9 The width is calculated as the FWHM divided by 2.35. cluster is, as usual, dened as the sum of the pulse height of the pixels. The same procedure has been applied, for comparison, also to dark frames.…”

Section: Single-photoelectron Detectionmentioning

confidence: 99%

The Megapixel EBCCD: A high-resolution imaging tube sensitive to single photons

Buontempo

Chiodi²,

Dalinenko

et al. 1998

Nuclear Instruments and Methods in Physics Research Section A:

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A h ybrid image-intensier tube, suitable for extremely low-light imaging, has been tested. This device is based on an Electron-Bombarded CCD chip (EBCCD) with 1024 1024 sensitive pixels. The tube, which has a photocathode diameter of 40 mm, is gateable and zoomable, with an image magnication varying from 0.62 to 1.3. The high gain (about 4000 collected electrons per photoelectron at the operational voltage of 15 kV) and the relatively low noise (180 electrons per pixel at 10 MHz pixelreadout frequency), allows single-photoelectron signals to be separated from noise with a signal-to-noise ratio greater than 10. By applying an appropriate threshold on the signal amplitude, the background can almost be eliminated, with a loss of few percent in single-photoelectron counting. High inner gain, low noise, single-photoelectron sensitivity, and high spatial resolution make the EBCCD imaging tube a unique device, attractive for many applications in high-energy physics, astrophysics, biomedical diagnostics.

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A CCD-based image recording system for the CTEM

Cited by 48 publications

References 10 publications

Applications of direct detection device in transmission electron microscopy

Applications of direct detection device in transmission electron microscopy

Transform-based backprojection for volume reconstruction of large format electron microscope tilt series

The Megapixel EBCCD: A high-resolution imaging tube sensitive to single photons

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