A cDNA clone from Zea mays endosperm sucrose synthetase mRNA

Geiser, Martin; Döring, Hans-Peter; Wöstemeyer, Johannes; Behrens, Ute; Tillmann, E.; Starlinger, Peter

doi:10.1093/nar/8.24.6175

Cited by 33 publications

(27 citation statements)

References 28 publications

(17 reference statements)

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“…After secondstrand synthesis with Pollk and size fractionation by 1.5% agarose gel electrophoresis the DNA of more than 500 bp was purified (Dretzen et al, 1981) and ligated into the Smal site of plasmid pUC12 and used to transform competent cells of Escherichia coli JM83 (Vieira and Messing, 1982). The library was screened using as a probé the nick-translated 32 P-labeled insert from pKS500 (Geiser et al, 1980).…”

Section: (B) Reagentsmentioning

confidence: 99%

“…The second gene, Ss2 or Css, is also expressed in several tissues but its expression is less affected by anaerobiosis (McCarty et al, 1986a). The Shl gene has been cloned and its entire DNA sequence has been determined (Geiser et al, 1980;Sheldon et al, 1983;Werr et al, 1985;Zack et al, 1986). Based on the ability of Shl probes to select by hybridization mRNA corresponding to the Css gene (McCormick et al, 1982), Css genomic clones have been isolated and characterized by McCarty et al (1986a) who have also located the Css (Ss2) gene near the centromere of chromosome 9 of maize, 32 map units from the Shl locus (McCarty et al, 1986b).…”

Section: Introductionmentioning

confidence: 99%

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Linked sucrose synthase genes in group-7 chromosomes in hexaploid wheat (Triticum aestivum L.)

Maraña

Garcı́a-Olmedo

Carbonero

1988

Gene

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SUMMARYA cDNA library from developing wheat endosperm was screened for sucrose-synthase clones using a maize cDNA probé corresponding to the Shl locus under non-stringent conditions. Five positive clones were isolated and initially classified into two types on the basis of their relative ability to hybridize with the probé and of their partial restriction maps. Determination of the nucleotide sequences indicated homology between the two types of wheat clones, with type 1 showing higher homology to the maize Shl locus than to type-2 sequences. The inserís cloned in plasmids pST8 (type 1) and pST3 (type 2) were used as probes to determine the chromosomal locations of the two types of genes. DNAs from compensated nulli-tetrasomic and ditelosomic lines of wheat cultivar Chinese Spring were cleaved with EcoRl and analysed in Southern blots. DNA segments of the two types were thus identified in the short arms of chromosomes 7A, 7D, and, possibly, 7B. The two types of linked loci have been designated Ssl and Ss2, respectively.

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Section: (B) Reagentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Linked sucrose synthase genes in group-7 chromosomes in hexaploid wheat (Triticum aestivum L.)

Maraña

Garcı́a-Olmedo

Carbonero

1988

Gene

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“…The regulation of SPS and SS activities has been thoroughly studied (5)(6)(7). The sequences of their genes have also been established in several plant species (8)(9)(10). On the other hand, there is little information on the specific phosphatase, SPP.…”

mentioning

confidence: 99%

Sucrose biosynthesis in a prokaryotic organism: Presence of two sucrose-phosphate synthases in Anabaena with remarkable differences compared with the plant enzymes

Porchia¹,

Salerno²

1996

Proc. Natl. Acad. Sci. U.S.A.

103

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Biosynthesis of sucrose-6-P catalyzed by sucrose-phosphate synthase (SPS), and the presence of sucrosephosphate phosphatase (SPP) leading to the formation of sucrose, have both been ascertained in a prokaryotic organism: Anabaena 7119, a filamentous heterocystic cyanobacterium. Two SPS activities (SPS-I and SPS-II) were isolated by ion-exchange chromatography and partially purified. Four remarkable differences between SPSs from Anabaena and those from higher plants were shown: substrate specificity, effect of divalent cations, native molecular mass, and oligomeric composition. Both SPS-I and SPS-II accept Fru-6-P (K m for SPS-I ‫؍‬ 0.8 ؎ 0.1 mM; K m for SPS-II ‫؍‬ 0.7 ؎ 0.1 mM) and UDP-Glc as substrates (K m for SPS-I ‫؍‬ 1.3 ؎ 0.4 mM; K m for SPS-II ‫؍‬ 4.6 ؎ 0.4 mM), but unlike higher plant enzymes, they are not specific for UDP-Glc. GDP-Glc and TDP-Glc are also SPS-I substrates (K m for GDP-Glc ‫؍‬ 1.2 ؎ 0.2 mM and K m for TDP-Glc ‫؍‬ 4.0 ؎ 0.4 mM), and ADP-Glc is used by SPS-II (K m for ADP-Glc ‫؍‬ 5.7 ؎ 0.7 mM). SPS-I has an absolute dependence toward divalent metal ions (Mg 2؉ or Mn 2؉ ) for catalytic activity, not found in plants. A strikingly smaller native molecular mass (between 45 and 47 kDa) was determined by gel filtration for both SPSs, which, when submitted to SDS͞PAGE, showed a monomeric composition. Cyanobacteria are, as far as the authors know, the most primitive organisms that are able to biosynthesize sucrose as higher plants do.

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“…Mycelia from 300-ml cultures were harvested by filtration on a Buchner funnel, washed twice with 500 ml cold 10 mM Tris/HCl, pH 8.0, 10 mM NaC1, 1 mM EDTA, and homogenized under liquid nitrogen with an Ultraturrax mixer three times for 40 s. Afer evaporation of liquid nitrogen, the resulting powder was suspended in 100 ml buffer, containing 0.15 M EDTA, 0.05 M Tris/HCl, pH 8.0, 0.02 M NaC1, 1% sodium dodecyl sulfate, and supplemented with 100 pg/ml proteinase K (Merck) [12]. After incubation for at least 3 h at 37°C with slow agitation, the homogenate was extracted twice with phenol, followed by two chloroform extractions and dialysis against 10 mM Tris/HCl pH 8.0, 10 mM EDTA, 20 mM NaCl.…”

Section: N a Preparationmentioning

confidence: 99%

“…Blotting of horizontal 0.8% agarose gels (13 x 18 cm) was carried out according to standard procedures [I 31; hybridization conditions were as described [12]. Sizes of restriction fragments were measured using phage 1 DNA, digested with EcoRI, AccI, or HindIII, as standard.…”

Section: Southern Blotting and Hybridizationmentioning

confidence: 99%

Strain‐dependent variation in ribosomal DNA arrangement in Absidia glauca

Wöstemeyer¹

1985

European Journal of Biochemistry

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Restriction analysis of total DNA from the zygomycete Absidia glauca reveals a pattern of prominent bands on a homogeneous background. By Southern blot analysis with 32P‐end‐labelled ribosomal RNA most of these bands could unequivocally be identified as repetitive copies of ribosomal DNA. There are marked differences in restriction patterns of rDNA between all seven strains tested, even of strains belonging to mating type pairs, presumably isolated from the same location. By using purified rRNAs as probes in hybridization experiments, evidence is presented that 5S rRNA is part of the ribosomal repetitive unit. A more detailed analysis of one strain pair [A. glauca CBS 100.48 (+)/101.48 (−)] provided evidence that the (+) strain, in addition to one rDNA repeat unit common to both strains, contains a second one, derived from the common form by a small deletion.

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A cDNA clone from Zea mays endosperm sucrose synthetase mRNA

Cited by 33 publications

References 28 publications

Linked sucrose synthase genes in group-7 chromosomes in hexaploid wheat (Triticum aestivum L.)

Linked sucrose synthase genes in group-7 chromosomes in hexaploid wheat (Triticum aestivum L.)

Sucrose biosynthesis in a prokaryotic organism: Presence of two sucrose-phosphate synthases in Anabaena with remarkable differences compared with the plant enzymes

Strain‐dependent variation in ribosomal DNA arrangement in Absidia glauca

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