A cDNA microarray for the three‐spined stickleback, <i>Gasterosteus aculeatus </i>L., and analysis of the interactive effects of oestradiol and dibenzanthracene exposures

Geoghegan, Frank; Katsiadaki, Ioanna; Williams, Tim D.; Chipman, J.K.

doi:10.1111/j.1095-8649.2008.01859.x

Cited by 32 publications

(16 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gene functional annotation and gene ontology and pathway analyses identified several biological and molecular processes that might be activated as a response to predation risk. The radiation of sticklebacks along with growing genomic resources [Kingsley and Peichel, 2007;Brown et al, 2008;Geoghegan et al, 2008;Williams et al, 2008;Leder et al, 2009] and its well-characterized biology, make this organism a suitable model system for investigating the molecular and physiological responses of the brain to ecologically relevant selection pressures that have influenced their evolution.…”

Section: Discussionmentioning

confidence: 99%

Brain Transcriptomic Response of Threespine Sticklebacks to Cues of a Predator

Sanogo

Hankison²,

Band³

et al. 2011

Brain Behav Evol

View full text Add to dashboard Cite

Predation pressure represents a strong selective force that influences the development and evolution of living organisms. An increasing number of studies have shown that both environmental and social factors, including exposure to predators, substantially shape the structure and function of the brain. However, our knowledge about the molecular mechanisms underlying the response of the brain to environmental stimuli is limited. In this study, we used whole-genome comparative oligonucleotide microarrays to investigate the brain transcriptomic response to cues of a predator in the threespine stickleback, Gasterosteus aculeatus. We found that repeated exposure to olfactory, visual and tactile cues of a predator (rainbow trout, Oncorrhynchus mykiss) for 6 days resulted in subtle but significant transcriptomic changes in the brain of sticklebacks. Gene functional analysis and gene ontology enrichment revealed that the majority of the transcripts differentially expressed between the fish exposed to cues of a predator and the control group were related to antigen processing and presentation involving the major histocompatibility complex, transmission of synaptic signals, brain metabolic processes, gene regulation and visual perception. The top four identified pathways were synaptic long-term depression, RAN signaling, relaxin signaling and phototransduction. Our study demonstrates that exposure of sticklebacks to cues of a predator results in the activation of a wide range of biological and molecular processes and lays the foundation for future investigations on the molecular factors that modulate the function and evolution of the brain in response to stressors.

show abstract

Section: Discussionmentioning

confidence: 99%

Brain Transcriptomic Response of Threespine Sticklebacks to Cues of a Predator

Sanogo

Hankison²,

Band³

et al. 2011

Brain Behav Evol

View full text Add to dashboard Cite

show abstract

“…Activity in the range 0.5 -10 pmol min -1 mg protein -1 has been reported for laboratory controls and sticklebacks from ostensibly unpolluted sites (Geoghegan et al, 2008;Sanchez et al, 2008a).…”

Section: Erod Activitymentioning

confidence: 99%

“…1, Table 1). On the R. Ray the first of these was immediately adjacent to the WWTW outfall (mean dry weather WWTW discharge = 0.51 m 3 /sec, data courtesy Thames Water; mean annual river discharge 1.34 m 3 /sec, Water Eaton Gauging fluorogenic probe were derived from previous work (Geoghegan et al, 2008). Primers CYP1AFP…”

Section: Sampling Sitesmentioning

confidence: 99%

Effects of sewage effluent remediation on body size, somatic RNA: DNA ratio, and markers of chemical exposure in three-spined sticklebacks

Pottinger

Cook

Jürgens

et al. 2011

Environment International

View full text Add to dashboard Cite

Body mass, fork length, RNA:DNA ratio, specific growth rate, and hepatic EROD activity and CYP1A expression, were measured in three-spined sticklebacks in the River Ray (south west England) at sites downstream of an urban waste water treatment works (WWTW) prior to, and following, remediation of the effluent with granular activated carbon (GAC) tertiary treatment.During the same two-year period fish were also sampled from a neighbouring reference river (R.Ock). The WWTW effluent elevated water temperatures and nutrient content in the R. Ray and rendered a direct comparison of fish populations in the two rivers untenable. Instead, the stability of population parameters within each river during matched pre-and post-remediation periods was compared. Stickleback populations in both rivers were annual but fish in the R. Ray spawned earlier and were larger than those in the R. Ock. In the R. Ray fish gained mass throughout the winter months whereas in the R. Ock growth was much reduced during this period. In fish from the R. Ray the somatic RNA:DNA ratio remained elevated during May-November after remediation, rather than declining as in the same period pre-remediation and as was the case for fish in the R. Ock during both periods. The specific growth rate of the first post-remediation generation of sticklebacks in the R. Ray was higher than that of the previous pre-remediation generation.Following remediation there was no decline in hepatic EROD activity or in the abundance of hepatic CYP1A transcripts in fish in the R. Ray suggesting that the primary route of exposure to contaminants for these fish was not via the water column, and that the change in performance of the fish post-remediation was not impeded by continued exposure to contaminants. Both EROD activity and CYP1A expression increased in fish in the R. Ock during the later stages of the study suggesting that the fish in this river were exposed to an unidentified contaminant episode. This may have been linked with the poorer performance of fish in the R. Ock during the post-remediation period. The improved performance of fish in the R. Ray suggest that there may be factors in good quality secondary treated sewage effluent which can adversely influence the performance of fish populations, directly or indirectly, and which can be removed by tertiary treatment.3

show abstract

“…Because of its geographical distribution, robustness, and sensitivity to androgen and estrogen exposure, stickleback is an attractive model in environmental monitoring of hormonally active pollutants (Andersson et al, 2007, Björkblom et al, 2007, Geoghegan et al, 2008, Katsiadaki et al, 2002). Stickleback responds to aryl hydrocarbon receptor (AHR) agonists with induction of cytochrome P4501A (CYP1A) mRNA and the CYP1A-catalyzed ethoxyresorufin- O -deethylase (EROD) activity (Andersson et al, 2007, Sanchez et al, 2007, Williams et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Cytochrome P450 1A, 1B, and 1C mRNA induction patterns in three-spined stickleback exposed to a transient and a persistent inducer

Gao

Brandt

Goldstone

et al. 2011

Comparative Biochemistry and Physiology Part C: Toxicology &

View full text Add to dashboard Cite

Cytochrome P450 1 (CYP1) mRNA induction patterns in three-spined stickleback (Gasterosteous aculeatus) were explored for use in environmental monitoring of aryl hydrocarbon receptor (AHR) agonists. The cDNAs of stickleback CYP1A, CYP1B1, CYP1C1, and CYP1C2 were cloned and their basal and induced expression patterns were determined in brain, gill, liver and kidney. Also, their induction time courses were compared after waterborne exposure to a transient (indigo) or a persistent (3,3′,4,4′,5-pentacholorbiphenyl PCB 126) AHR agonist. The cloned stickleback CYP1s exhibited a high amino acid sequence identity compared with their zebrafish orthologs and their constitutive tissue distribution patterns largely agree with those reported in other species. PCB 126 (100 nM) induced different CYP1 expression patterns in the four tissues, suggesting tissue-specific regulation. Both indigo (1 nM) and PCB 126 (10 nM) induced a strong CYP1 expression in gills. However, while PCB 126 gave rise to a high and persistent induction in gills and liver, induction by indigo was transient in both organs. The number of putative dioxin response elements found in each CYP1 gene promoter roughly reflected the induction levels of the genes. The high responsiveness of CYP1A, CYP1B1, and CYP1C1 observed in several organs suggests that three-spined stickleback is suitable for monitoring of pollution with AHR agonists.

show abstract

A cDNA microarray for the three‐spined stickleback, Gasterosteus aculeatus L., and analysis of the interactive effects of oestradiol and dibenzanthracene exposures

Cited by 32 publications

References 44 publications

Brain Transcriptomic Response of Threespine Sticklebacks to Cues of a Predator

Brain Transcriptomic Response of Threespine Sticklebacks to Cues of a Predator

Effects of sewage effluent remediation on body size, somatic RNA: DNA ratio, and markers of chemical exposure in three-spined sticklebacks

Cytochrome P450 1A, 1B, and 1C mRNA induction patterns in three-spined stickleback exposed to a transient and a persistent inducer

Contact Info

Product

Resources

About