A cell culture analogue of rodent physiology: Application to naphthalene toxicology

Building benchtop human models

“…For example, the pesticide naphthalene, found in mothballs, can cause headaches, nausea, or anemia, although it's not clear exactly how naphthalene sickens people. Michael Shuler, a biomedical engineer at Cornell University in Ithaca, New York, used a model with cells from both the liver and lung to find that the liver cells turn naphthalene into naphthoquinone, and this compound in turn damages lung cells (3). A model with only liver or only lung tissue could never have illustrated this process.…”

Microfluidic Bioreactors for Cell Culturing: A Review

“…Such physical models can be used as alternative methods to predict human response by exposure to chemicals or pharmaceuticals [252]. The basic concepts of CCA devices are described in [253][254]. Viravaidya et al [255], developed a miniaturised CCA device for culturing liver, lung and fat cells in different interconnected compartments to mimic the physiological features such as residence time, of the circulation and exchange of metabolites in the body.…”

Modeling Life

“…Fluid flow to each compartment and the size of the compartment are in the same ratio as in the human body which should result in similar rates of reaction. We called the first device of this type a cell culture analog (CCA) and was described in Sweeny et al 24 We then constructed our first micro scale device, a microCCA and described it in 2004. 17 The micro scale makes it feasible to run multiple units relatively cheap.…”